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Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow

Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bo...

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Autores principales: Mattos, Erika Bertozzi de Aquino, Pereira, Patricia Ribeiro, Mérida, Lyris Anunciata Demétrio, Corrêa, Anna Carolina Nitzsche Teixeira Fernandes, Freire, Maria Paula Vigna, Paschoalin, Vania Margaret Flosi, Teixeira, Gerlinde Agate Platais Brasil, Pinho, Maria de Fátima Brandão, Verícimo, Maurício Afonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001523/
https://www.ncbi.nlm.nih.gov/pubmed/33800086
http://dx.doi.org/10.3390/pharmaceutics13030350
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author Mattos, Erika Bertozzi de Aquino
Pereira, Patricia Ribeiro
Mérida, Lyris Anunciata Demétrio
Corrêa, Anna Carolina Nitzsche Teixeira Fernandes
Freire, Maria Paula Vigna
Paschoalin, Vania Margaret Flosi
Teixeira, Gerlinde Agate Platais Brasil
Pinho, Maria de Fátima Brandão
Verícimo, Maurício Afonso
author_facet Mattos, Erika Bertozzi de Aquino
Pereira, Patricia Ribeiro
Mérida, Lyris Anunciata Demétrio
Corrêa, Anna Carolina Nitzsche Teixeira Fernandes
Freire, Maria Paula Vigna
Paschoalin, Vania Margaret Flosi
Teixeira, Gerlinde Agate Platais Brasil
Pinho, Maria de Fátima Brandão
Verícimo, Maurício Afonso
author_sort Mattos, Erika Bertozzi de Aquino
collection PubMed
description Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bone marrow hematopoietic population, murine cells were stimulated with tarin combined with a highly enriched conditioned medium containing either IL-3 or GM-CSF. Cells challenged with tarin proliferated in a dose-dependent manner, evidenced by the increase in cell density and number of clusters and colonies. Tarin exhibited a cytokine-mimetic effect similar to IL-3 and GM-CSF, increasing granulocytic cell lineage percentages, demonstrated by an increase in the relative percentage of Gr-1+ cells. Tarin does not increase lymphocytic lineages, but phenotyping revealed that the relative percentage of CD3+ cells was increased with a concomitant decrease in CD19+ and IL-7Rα+ cells. Most bone marrow cells were stained with tarin-FITC, indicating non-selective tarin binding, a phenomenon that must still be elucidated. In conclusion, taro corms contain an immunomodulatory lectin able to boost the immune system by promoting myeloid and lymphoid hematopoietic progenitor cell proliferation and differentiation.
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spelling pubmed-80015232021-03-28 Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow Mattos, Erika Bertozzi de Aquino Pereira, Patricia Ribeiro Mérida, Lyris Anunciata Demétrio Corrêa, Anna Carolina Nitzsche Teixeira Fernandes Freire, Maria Paula Vigna Paschoalin, Vania Margaret Flosi Teixeira, Gerlinde Agate Platais Brasil Pinho, Maria de Fátima Brandão Verícimo, Maurício Afonso Pharmaceutics Article Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bone marrow hematopoietic population, murine cells were stimulated with tarin combined with a highly enriched conditioned medium containing either IL-3 or GM-CSF. Cells challenged with tarin proliferated in a dose-dependent manner, evidenced by the increase in cell density and number of clusters and colonies. Tarin exhibited a cytokine-mimetic effect similar to IL-3 and GM-CSF, increasing granulocytic cell lineage percentages, demonstrated by an increase in the relative percentage of Gr-1+ cells. Tarin does not increase lymphocytic lineages, but phenotyping revealed that the relative percentage of CD3+ cells was increased with a concomitant decrease in CD19+ and IL-7Rα+ cells. Most bone marrow cells were stained with tarin-FITC, indicating non-selective tarin binding, a phenomenon that must still be elucidated. In conclusion, taro corms contain an immunomodulatory lectin able to boost the immune system by promoting myeloid and lymphoid hematopoietic progenitor cell proliferation and differentiation. MDPI 2021-03-07 /pmc/articles/PMC8001523/ /pubmed/33800086 http://dx.doi.org/10.3390/pharmaceutics13030350 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Mattos, Erika Bertozzi de Aquino
Pereira, Patricia Ribeiro
Mérida, Lyris Anunciata Demétrio
Corrêa, Anna Carolina Nitzsche Teixeira Fernandes
Freire, Maria Paula Vigna
Paschoalin, Vania Margaret Flosi
Teixeira, Gerlinde Agate Platais Brasil
Pinho, Maria de Fátima Brandão
Verícimo, Maurício Afonso
Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title_full Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title_fullStr Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title_full_unstemmed Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title_short Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
title_sort taro lectin can act as a cytokine-mimetic compound, stimulating myeloid and t lymphocyte lineages and protecting progenitors in murine bone marrow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001523/
https://www.ncbi.nlm.nih.gov/pubmed/33800086
http://dx.doi.org/10.3390/pharmaceutics13030350
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