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Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow
Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001523/ https://www.ncbi.nlm.nih.gov/pubmed/33800086 http://dx.doi.org/10.3390/pharmaceutics13030350 |
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author | Mattos, Erika Bertozzi de Aquino Pereira, Patricia Ribeiro Mérida, Lyris Anunciata Demétrio Corrêa, Anna Carolina Nitzsche Teixeira Fernandes Freire, Maria Paula Vigna Paschoalin, Vania Margaret Flosi Teixeira, Gerlinde Agate Platais Brasil Pinho, Maria de Fátima Brandão Verícimo, Maurício Afonso |
author_facet | Mattos, Erika Bertozzi de Aquino Pereira, Patricia Ribeiro Mérida, Lyris Anunciata Demétrio Corrêa, Anna Carolina Nitzsche Teixeira Fernandes Freire, Maria Paula Vigna Paschoalin, Vania Margaret Flosi Teixeira, Gerlinde Agate Platais Brasil Pinho, Maria de Fátima Brandão Verícimo, Maurício Afonso |
author_sort | Mattos, Erika Bertozzi de Aquino |
collection | PubMed |
description | Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bone marrow hematopoietic population, murine cells were stimulated with tarin combined with a highly enriched conditioned medium containing either IL-3 or GM-CSF. Cells challenged with tarin proliferated in a dose-dependent manner, evidenced by the increase in cell density and number of clusters and colonies. Tarin exhibited a cytokine-mimetic effect similar to IL-3 and GM-CSF, increasing granulocytic cell lineage percentages, demonstrated by an increase in the relative percentage of Gr-1+ cells. Tarin does not increase lymphocytic lineages, but phenotyping revealed that the relative percentage of CD3+ cells was increased with a concomitant decrease in CD19+ and IL-7Rα+ cells. Most bone marrow cells were stained with tarin-FITC, indicating non-selective tarin binding, a phenomenon that must still be elucidated. In conclusion, taro corms contain an immunomodulatory lectin able to boost the immune system by promoting myeloid and lymphoid hematopoietic progenitor cell proliferation and differentiation. |
format | Online Article Text |
id | pubmed-8001523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80015232021-03-28 Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow Mattos, Erika Bertozzi de Aquino Pereira, Patricia Ribeiro Mérida, Lyris Anunciata Demétrio Corrêa, Anna Carolina Nitzsche Teixeira Fernandes Freire, Maria Paula Vigna Paschoalin, Vania Margaret Flosi Teixeira, Gerlinde Agate Platais Brasil Pinho, Maria de Fátima Brandão Verícimo, Maurício Afonso Pharmaceutics Article Taro (Colocasia esculenta) corm is traditionally consumed as a medicinal plant to stimulate immune responses and restore a health status. Tarin, a taro lectin, is considered responsible for the immunomodulatory effects of taro. In the present study, in order to investigate the effects of tarin on bone marrow hematopoietic population, murine cells were stimulated with tarin combined with a highly enriched conditioned medium containing either IL-3 or GM-CSF. Cells challenged with tarin proliferated in a dose-dependent manner, evidenced by the increase in cell density and number of clusters and colonies. Tarin exhibited a cytokine-mimetic effect similar to IL-3 and GM-CSF, increasing granulocytic cell lineage percentages, demonstrated by an increase in the relative percentage of Gr-1+ cells. Tarin does not increase lymphocytic lineages, but phenotyping revealed that the relative percentage of CD3+ cells was increased with a concomitant decrease in CD19+ and IL-7Rα+ cells. Most bone marrow cells were stained with tarin-FITC, indicating non-selective tarin binding, a phenomenon that must still be elucidated. In conclusion, taro corms contain an immunomodulatory lectin able to boost the immune system by promoting myeloid and lymphoid hematopoietic progenitor cell proliferation and differentiation. MDPI 2021-03-07 /pmc/articles/PMC8001523/ /pubmed/33800086 http://dx.doi.org/10.3390/pharmaceutics13030350 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Mattos, Erika Bertozzi de Aquino Pereira, Patricia Ribeiro Mérida, Lyris Anunciata Demétrio Corrêa, Anna Carolina Nitzsche Teixeira Fernandes Freire, Maria Paula Vigna Paschoalin, Vania Margaret Flosi Teixeira, Gerlinde Agate Platais Brasil Pinho, Maria de Fátima Brandão Verícimo, Maurício Afonso Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title | Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title_full | Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title_fullStr | Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title_full_unstemmed | Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title_short | Taro Lectin Can Act as a Cytokine-Mimetic Compound, Stimulating Myeloid and T Lymphocyte Lineages and Protecting Progenitors in Murine Bone Marrow |
title_sort | taro lectin can act as a cytokine-mimetic compound, stimulating myeloid and t lymphocyte lineages and protecting progenitors in murine bone marrow |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001523/ https://www.ncbi.nlm.nih.gov/pubmed/33800086 http://dx.doi.org/10.3390/pharmaceutics13030350 |
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