A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity

Cas3 is a ssDNA-targeting nuclease-helicase essential for class 1 prokaryotic CRISPR immunity systems, which has been utilized for genome editing in human cells. Cas3-DNA crystal structures show that ssDNA follows a pathway from helicase domains into a HD-nuclease active site, requiring protein conf...

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Detalles Bibliográficos
Autores principales: He, Liu, Matošević, Zoe Jelić, Mitić, Damjan, Markulin, Dora, Killelea, Tom, Matković, Marija, Bertoša, Branimir, Ivančić-Baće, Ivana, Bolt, Edward L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001533/
https://www.ncbi.nlm.nih.gov/pubmed/33799639
http://dx.doi.org/10.3390/ijms22062848

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