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Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury

Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and...

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Autores principales: Cho, Byoung Ok, Kim, Jang Hoon, Che, Denis Nchang, Kang, Hyun Ju, Shin, Jae Young, Hao, Suping, Park, Ji Hyeon, Wang, Feng, Lee, Yun Ji, Jang, Seon Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001553/
https://www.ncbi.nlm.nih.gov/pubmed/33804228
http://dx.doi.org/10.3390/molecules26061635
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author Cho, Byoung Ok
Kim, Jang Hoon
Che, Denis Nchang
Kang, Hyun Ju
Shin, Jae Young
Hao, Suping
Park, Ji Hyeon
Wang, Feng
Lee, Yun Ji
Jang, Seon Il
author_facet Cho, Byoung Ok
Kim, Jang Hoon
Che, Denis Nchang
Kang, Hyun Ju
Shin, Jae Young
Hao, Suping
Park, Ji Hyeon
Wang, Feng
Lee, Yun Ji
Jang, Seon Il
author_sort Cho, Byoung Ok
collection PubMed
description Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress.
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spelling pubmed-80015532021-03-28 Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury Cho, Byoung Ok Kim, Jang Hoon Che, Denis Nchang Kang, Hyun Ju Shin, Jae Young Hao, Suping Park, Ji Hyeon Wang, Feng Lee, Yun Ji Jang, Seon Il Molecules Article Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress. MDPI 2021-03-15 /pmc/articles/PMC8001553/ /pubmed/33804228 http://dx.doi.org/10.3390/molecules26061635 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cho, Byoung Ok
Kim, Jang Hoon
Che, Denis Nchang
Kang, Hyun Ju
Shin, Jae Young
Hao, Suping
Park, Ji Hyeon
Wang, Feng
Lee, Yun Ji
Jang, Seon Il
Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title_full Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title_fullStr Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title_full_unstemmed Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title_short Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
title_sort kushenol c prevents tert-butyl hydroperoxide and acetaminophen-induced liver injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001553/
https://www.ncbi.nlm.nih.gov/pubmed/33804228
http://dx.doi.org/10.3390/molecules26061635
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