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Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001553/ https://www.ncbi.nlm.nih.gov/pubmed/33804228 http://dx.doi.org/10.3390/molecules26061635 |
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author | Cho, Byoung Ok Kim, Jang Hoon Che, Denis Nchang Kang, Hyun Ju Shin, Jae Young Hao, Suping Park, Ji Hyeon Wang, Feng Lee, Yun Ji Jang, Seon Il |
author_facet | Cho, Byoung Ok Kim, Jang Hoon Che, Denis Nchang Kang, Hyun Ju Shin, Jae Young Hao, Suping Park, Ji Hyeon Wang, Feng Lee, Yun Ji Jang, Seon Il |
author_sort | Cho, Byoung Ok |
collection | PubMed |
description | Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress. |
format | Online Article Text |
id | pubmed-8001553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80015532021-03-28 Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury Cho, Byoung Ok Kim, Jang Hoon Che, Denis Nchang Kang, Hyun Ju Shin, Jae Young Hao, Suping Park, Ji Hyeon Wang, Feng Lee, Yun Ji Jang, Seon Il Molecules Article Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress. MDPI 2021-03-15 /pmc/articles/PMC8001553/ /pubmed/33804228 http://dx.doi.org/10.3390/molecules26061635 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cho, Byoung Ok Kim, Jang Hoon Che, Denis Nchang Kang, Hyun Ju Shin, Jae Young Hao, Suping Park, Ji Hyeon Wang, Feng Lee, Yun Ji Jang, Seon Il Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title | Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title_full | Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title_fullStr | Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title_full_unstemmed | Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title_short | Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury |
title_sort | kushenol c prevents tert-butyl hydroperoxide and acetaminophen-induced liver injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001553/ https://www.ncbi.nlm.nih.gov/pubmed/33804228 http://dx.doi.org/10.3390/molecules26061635 |
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