Comparative Transcriptome Analysis Identifies Candidate Genes Related to Black-Spotted Pattern Formation in Spotted Scat (Scatophagus argus)

SIMPLE SUMMARY: Spotted scat (Scatophagus argus) is a commercially important marine aquaculture and ornamental fish species in China and East Asian countries. There are dozens of black spots on each side of the body, and the caudal fin, which is yellow and black, is appreciated in ornamental fish markets. To explore the genetic mechanisms of its pattern formation, we found 2357 differentially expressed genes (DEGs) by comparing the transcriptome in the black-spotted skin, non-spotted skin and caudal fin in S. argus. The results will expand our knowledge about the molecular mechanism of important genes and pathways associated with pigment pattern formation and provide a certain theoretical basis for the molecular breeding in S. argus. ABSTRACT: Spotted scat (Scatophagus argus) is an economically important marine aquaculture and ornamental fish species in Asia, especially in southeast China. In this study, skin transcriptomes of S. argus were obtained for three types of skin, including black-spotted skin (A), non-spotted skin (B) and caudal fin (C). A total of nine complementary DNA (cDNA) libraries were obtained by Illumina sequencing. Bioinformatics analysis revealed that 1358, 2086 and 487 genes were differentially expressed between A and B, A and C, and B and C, respectively. The results revealed that there were 134 common significantly differentially expressed genes (DEGs) and several key genes related to pigment synthesis and pigmentation, including tyrp1, mitf, pmel, slc7a2, tjp1, hsp70 and mart-1. Of these, some DEGs were associated with pigmentation-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as tyrosine metabolism, melanogenesis, the Wnt signaling pathway and the mitogen-activated protein kinase (MAPK) signaling pathway. The results will facilitate understanding the molecular mechanisms of skin pigmentation differentiation in S. argus and provide valuable information for skin coloration, especially the formation of spotted patterns on other marine fish species.