Cargando…

Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics

This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers...

Descripción completa

Detalles Bibliográficos
Autores principales: Matbaechi Ettehad, Honeyeh, Wenger, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001765/
https://www.ncbi.nlm.nih.gov/pubmed/33800809
http://dx.doi.org/10.3390/mi12030270
_version_ 1783671306666901504
author Matbaechi Ettehad, Honeyeh
Wenger, Christian
author_facet Matbaechi Ettehad, Honeyeh
Wenger, Christian
author_sort Matbaechi Ettehad, Honeyeh
collection PubMed
description This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers excellent benefits in terms of cost, operational power, and especially easy electrode integration with the CMOS architecture, and requiring label-free sample preparation. This can increase the likeliness of using DEP in practical settings. In this work the DEP force was generated using an interdigitated electrode arrays (IDEs) placed on the bottom of a CMOS-based silicon microfluidic channel. This system was primarily used for the immobilization of yeast cells using DEP. This study validated the system for cell separation applications based on the distinct responses of live and dead cells and their surrounding media. The findings confirmed the device’s capability for efficient, rapid and selective cell separation. The viability of this CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and compatibility of the dielectrophoretic structure with CMOS production line and electronics, enabling its future commercially mass production.
format Online
Article
Text
id pubmed-8001765
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80017652021-03-28 Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics Matbaechi Ettehad, Honeyeh Wenger, Christian Micromachines (Basel) Article This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers excellent benefits in terms of cost, operational power, and especially easy electrode integration with the CMOS architecture, and requiring label-free sample preparation. This can increase the likeliness of using DEP in practical settings. In this work the DEP force was generated using an interdigitated electrode arrays (IDEs) placed on the bottom of a CMOS-based silicon microfluidic channel. This system was primarily used for the immobilization of yeast cells using DEP. This study validated the system for cell separation applications based on the distinct responses of live and dead cells and their surrounding media. The findings confirmed the device’s capability for efficient, rapid and selective cell separation. The viability of this CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and compatibility of the dielectrophoretic structure with CMOS production line and electronics, enabling its future commercially mass production. MDPI 2021-03-06 /pmc/articles/PMC8001765/ /pubmed/33800809 http://dx.doi.org/10.3390/mi12030270 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Matbaechi Ettehad, Honeyeh
Wenger, Christian
Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title_full Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title_fullStr Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title_full_unstemmed Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title_short Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
title_sort characterization and separation of live and dead yeast cells using cmos-based dep microfluidics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001765/
https://www.ncbi.nlm.nih.gov/pubmed/33800809
http://dx.doi.org/10.3390/mi12030270
work_keys_str_mv AT matbaechiettehadhoneyeh characterizationandseparationofliveanddeadyeastcellsusingcmosbaseddepmicrofluidics
AT wengerchristian characterizationandseparationofliveanddeadyeastcellsusingcmosbaseddepmicrofluidics