Cargando…
Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics
This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001765/ https://www.ncbi.nlm.nih.gov/pubmed/33800809 http://dx.doi.org/10.3390/mi12030270 |
_version_ | 1783671306666901504 |
---|---|
author | Matbaechi Ettehad, Honeyeh Wenger, Christian |
author_facet | Matbaechi Ettehad, Honeyeh Wenger, Christian |
author_sort | Matbaechi Ettehad, Honeyeh |
collection | PubMed |
description | This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers excellent benefits in terms of cost, operational power, and especially easy electrode integration with the CMOS architecture, and requiring label-free sample preparation. This can increase the likeliness of using DEP in practical settings. In this work the DEP force was generated using an interdigitated electrode arrays (IDEs) placed on the bottom of a CMOS-based silicon microfluidic channel. This system was primarily used for the immobilization of yeast cells using DEP. This study validated the system for cell separation applications based on the distinct responses of live and dead cells and their surrounding media. The findings confirmed the device’s capability for efficient, rapid and selective cell separation. The viability of this CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and compatibility of the dielectrophoretic structure with CMOS production line and electronics, enabling its future commercially mass production. |
format | Online Article Text |
id | pubmed-8001765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80017652021-03-28 Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics Matbaechi Ettehad, Honeyeh Wenger, Christian Micromachines (Basel) Article This study aims at developing a miniaturized CMOS integrated silicon-based microfluidic system, compatible with a standard CMOS process, to enable the characterization, and separation of live and dead yeast cells (as model bio-particle organisms) in a cell mixture using the DEP technique. DEP offers excellent benefits in terms of cost, operational power, and especially easy electrode integration with the CMOS architecture, and requiring label-free sample preparation. This can increase the likeliness of using DEP in practical settings. In this work the DEP force was generated using an interdigitated electrode arrays (IDEs) placed on the bottom of a CMOS-based silicon microfluidic channel. This system was primarily used for the immobilization of yeast cells using DEP. This study validated the system for cell separation applications based on the distinct responses of live and dead cells and their surrounding media. The findings confirmed the device’s capability for efficient, rapid and selective cell separation. The viability of this CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and compatibility of the dielectrophoretic structure with CMOS production line and electronics, enabling its future commercially mass production. MDPI 2021-03-06 /pmc/articles/PMC8001765/ /pubmed/33800809 http://dx.doi.org/10.3390/mi12030270 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Matbaechi Ettehad, Honeyeh Wenger, Christian Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title | Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title_full | Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title_fullStr | Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title_full_unstemmed | Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title_short | Characterization and Separation of Live and Dead Yeast Cells Using CMOS-Based DEP Microfluidics |
title_sort | characterization and separation of live and dead yeast cells using cmos-based dep microfluidics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001765/ https://www.ncbi.nlm.nih.gov/pubmed/33800809 http://dx.doi.org/10.3390/mi12030270 |
work_keys_str_mv | AT matbaechiettehadhoneyeh characterizationandseparationofliveanddeadyeastcellsusingcmosbaseddepmicrofluidics AT wengerchristian characterizationandseparationofliveanddeadyeastcellsusingcmosbaseddepmicrofluidics |