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In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue...

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Autores principales: Cunha, Fernando Bento, Pomini, Karina Torres, Plepis, Ana Maria de Guzzi, Martins, Virgínia da Conceição Amaro, Machado, Eduardo Gomes, de Moraes, Renato, Munhoz, Marcelo de Azevedo e Souza, Machado, Michela Vanessa Ribeiro, Duarte, Marco Antonio Hungaro, Alcalde, Murilo Priori, Buchaim, Daniela Vieira, Buchaim, Rogério Leone, Fernandes, Victor Augusto Ramos, Pereira, Eliana de Souza Bastos Mazuqueli, Pelegrine, André Antonio, da Cunha, Marcelo Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002007/
https://www.ncbi.nlm.nih.gov/pubmed/33805847
http://dx.doi.org/10.3390/molecules26061598
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author Cunha, Fernando Bento
Pomini, Karina Torres
Plepis, Ana Maria de Guzzi
Martins, Virgínia da Conceição Amaro
Machado, Eduardo Gomes
de Moraes, Renato
Munhoz, Marcelo de Azevedo e Souza
Machado, Michela Vanessa Ribeiro
Duarte, Marco Antonio Hungaro
Alcalde, Murilo Priori
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
Fernandes, Victor Augusto Ramos
Pereira, Eliana de Souza Bastos Mazuqueli
Pelegrine, André Antonio
da Cunha, Marcelo Rodrigues
author_facet Cunha, Fernando Bento
Pomini, Karina Torres
Plepis, Ana Maria de Guzzi
Martins, Virgínia da Conceição Amaro
Machado, Eduardo Gomes
de Moraes, Renato
Munhoz, Marcelo de Azevedo e Souza
Machado, Michela Vanessa Ribeiro
Duarte, Marco Antonio Hungaro
Alcalde, Murilo Priori
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
Fernandes, Victor Augusto Ramos
Pereira, Eliana de Souza Bastos Mazuqueli
Pelegrine, André Antonio
da Cunha, Marcelo Rodrigues
author_sort Cunha, Fernando Bento
collection PubMed
description Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).
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spelling pubmed-80020072021-03-28 In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process Cunha, Fernando Bento Pomini, Karina Torres Plepis, Ana Maria de Guzzi Martins, Virgínia da Conceição Amaro Machado, Eduardo Gomes de Moraes, Renato Munhoz, Marcelo de Azevedo e Souza Machado, Michela Vanessa Ribeiro Duarte, Marco Antonio Hungaro Alcalde, Murilo Priori Buchaim, Daniela Vieira Buchaim, Rogério Leone Fernandes, Victor Augusto Ramos Pereira, Eliana de Souza Bastos Mazuqueli Pelegrine, André Antonio da Cunha, Marcelo Rodrigues Molecules Article Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25). MDPI 2021-03-13 /pmc/articles/PMC8002007/ /pubmed/33805847 http://dx.doi.org/10.3390/molecules26061598 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cunha, Fernando Bento
Pomini, Karina Torres
Plepis, Ana Maria de Guzzi
Martins, Virgínia da Conceição Amaro
Machado, Eduardo Gomes
de Moraes, Renato
Munhoz, Marcelo de Azevedo e Souza
Machado, Michela Vanessa Ribeiro
Duarte, Marco Antonio Hungaro
Alcalde, Murilo Priori
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
Fernandes, Victor Augusto Ramos
Pereira, Eliana de Souza Bastos Mazuqueli
Pelegrine, André Antonio
da Cunha, Marcelo Rodrigues
In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title_full In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title_fullStr In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title_full_unstemmed In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title_short In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
title_sort in vivo biological behavior of polymer scaffolds of natural origin in the bone repair process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002007/
https://www.ncbi.nlm.nih.gov/pubmed/33805847
http://dx.doi.org/10.3390/molecules26061598
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