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A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure

Background: Arterial hypertension is the most important risk factor for cardiovascular diseases, myocardial infarction, heart failure, renal failure and peripheral vascular disease. In the last decade, milk-derived bioactive peptides have attracted attention for their beneficial cardiovascular prope...

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Autores principales: Carrizzo, Albino, Basilicata, Manuela Giovanna, Pepe, Giacomo, Sørensen, Kasper K., Ciccarelli, Michele, Sarno, Veronica Di, Damato, Antonio, Venturini, Eleonora, Borrelli, Anna, Musella, Simona, Abate, Mario, Pietro, Paola Di, Ostacolo, Carmine, Campiglia, Pietro, Vecchione, Carmine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002072/
https://www.ncbi.nlm.nih.gov/pubmed/33809389
http://dx.doi.org/10.3390/antiox10030441
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author Carrizzo, Albino
Basilicata, Manuela Giovanna
Pepe, Giacomo
Sørensen, Kasper K.
Ciccarelli, Michele
Sarno, Veronica Di
Damato, Antonio
Venturini, Eleonora
Borrelli, Anna
Musella, Simona
Abate, Mario
Pietro, Paola Di
Ostacolo, Carmine
Campiglia, Pietro
Vecchione, Carmine
author_facet Carrizzo, Albino
Basilicata, Manuela Giovanna
Pepe, Giacomo
Sørensen, Kasper K.
Ciccarelli, Michele
Sarno, Veronica Di
Damato, Antonio
Venturini, Eleonora
Borrelli, Anna
Musella, Simona
Abate, Mario
Pietro, Paola Di
Ostacolo, Carmine
Campiglia, Pietro
Vecchione, Carmine
author_sort Carrizzo, Albino
collection PubMed
description Background: Arterial hypertension is the most important risk factor for cardiovascular diseases, myocardial infarction, heart failure, renal failure and peripheral vascular disease. In the last decade, milk-derived bioactive peptides have attracted attention for their beneficial cardiovascular properties. Methods: Here, we combined in vitro chemical assay such as LC-MS/MS analysis of buffalo ice cream, ex vivo vascular studies evaluating endothelial and smooth muscle responses using pressure myograph, and translational assay testing in vivo the vascular actions of PG1 administration in murine models. Results: We demonstrate that a novel buffalo ice-cream-derived pentapeptide “QKEPM”, namely PG1, is a stable peptide that can be obtained at higher concentration after gastro-intestinal digestions (GID) of buffalo ice-cream (BIC). It owns potent vascular effect in counteract the effects of angiotensin II-evoked vasoconstriction and high blood pressure levels. Its effects are mediated by the inhibitory effect on AT1 receptor leading to a downregulation of p-ERK½/Rac1-GTP and consequent reduction of oxidative stress. Conclusions: These results strongly candidate PG1, as a novel bioactive peptide for the prevention and management of hypertension, thus expanding the armamentarium of preventive strategies aimed at reducing the incidence and progression of hypertension and its related cardiovascular complications.
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spelling pubmed-80020722021-03-28 A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure Carrizzo, Albino Basilicata, Manuela Giovanna Pepe, Giacomo Sørensen, Kasper K. Ciccarelli, Michele Sarno, Veronica Di Damato, Antonio Venturini, Eleonora Borrelli, Anna Musella, Simona Abate, Mario Pietro, Paola Di Ostacolo, Carmine Campiglia, Pietro Vecchione, Carmine Antioxidants (Basel) Article Background: Arterial hypertension is the most important risk factor for cardiovascular diseases, myocardial infarction, heart failure, renal failure and peripheral vascular disease. In the last decade, milk-derived bioactive peptides have attracted attention for their beneficial cardiovascular properties. Methods: Here, we combined in vitro chemical assay such as LC-MS/MS analysis of buffalo ice cream, ex vivo vascular studies evaluating endothelial and smooth muscle responses using pressure myograph, and translational assay testing in vivo the vascular actions of PG1 administration in murine models. Results: We demonstrate that a novel buffalo ice-cream-derived pentapeptide “QKEPM”, namely PG1, is a stable peptide that can be obtained at higher concentration after gastro-intestinal digestions (GID) of buffalo ice-cream (BIC). It owns potent vascular effect in counteract the effects of angiotensin II-evoked vasoconstriction and high blood pressure levels. Its effects are mediated by the inhibitory effect on AT1 receptor leading to a downregulation of p-ERK½/Rac1-GTP and consequent reduction of oxidative stress. Conclusions: These results strongly candidate PG1, as a novel bioactive peptide for the prevention and management of hypertension, thus expanding the armamentarium of preventive strategies aimed at reducing the incidence and progression of hypertension and its related cardiovascular complications. MDPI 2021-03-12 /pmc/articles/PMC8002072/ /pubmed/33809389 http://dx.doi.org/10.3390/antiox10030441 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Carrizzo, Albino
Basilicata, Manuela Giovanna
Pepe, Giacomo
Sørensen, Kasper K.
Ciccarelli, Michele
Sarno, Veronica Di
Damato, Antonio
Venturini, Eleonora
Borrelli, Anna
Musella, Simona
Abate, Mario
Pietro, Paola Di
Ostacolo, Carmine
Campiglia, Pietro
Vecchione, Carmine
A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title_full A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title_fullStr A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title_full_unstemmed A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title_short A Novel Vasoactive Peptide “PG1” from Buffalo Ice-Cream Protects from Angiotensin-Evoked High Blood Pressure
title_sort novel vasoactive peptide “pg1” from buffalo ice-cream protects from angiotensin-evoked high blood pressure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002072/
https://www.ncbi.nlm.nih.gov/pubmed/33809389
http://dx.doi.org/10.3390/antiox10030441
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