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Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has seen only modest improvements in patient survival rate over the past few decades. PDAC is highly aggressive and resistant to chemotherapy, owing to the presence of a dense and hypovascularized fibrotic tissu...

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Autores principales: Chang, Chun-Yi, Lin, Chien-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002168/
https://www.ncbi.nlm.nih.gov/pubmed/33805737
http://dx.doi.org/10.3390/bioengineering8030037
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author Chang, Chun-Yi
Lin, Chien-Chi
author_facet Chang, Chun-Yi
Lin, Chien-Chi
author_sort Chang, Chun-Yi
collection PubMed
description Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has seen only modest improvements in patient survival rate over the past few decades. PDAC is highly aggressive and resistant to chemotherapy, owing to the presence of a dense and hypovascularized fibrotic tissue, which is composed of stromal cells and extracellular matrices. Increase deposition and crosslinking of matrices by stromal cells lead to a heterogeneous microenvironment that aids in PDAC development. In the past decade, various hydrogel-based, in vitro tumor models have been developed to mimic and recapitulate aspects of the tumor microenvironment in PDAC. Advances in hydrogel chemistry and engineering should provide a venue for discovering new insights regarding how matrix properties govern PDAC cell growth, migration, invasion, and drug resistance. These engineered hydrogels are ideal for understanding how variation in matrix properties contributes to the progressiveness of cancer cells, including durotaxis, the directional migration of cells in response to a stiffness gradient. This review surveys the various hydrogel-based, in vitro tumor models and the methods to generate gradient stiffness for studying migration and other cancer cell fate processes in PDAC.
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spelling pubmed-80021682021-03-28 Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate Chang, Chun-Yi Lin, Chien-Chi Bioengineering (Basel) Review Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has seen only modest improvements in patient survival rate over the past few decades. PDAC is highly aggressive and resistant to chemotherapy, owing to the presence of a dense and hypovascularized fibrotic tissue, which is composed of stromal cells and extracellular matrices. Increase deposition and crosslinking of matrices by stromal cells lead to a heterogeneous microenvironment that aids in PDAC development. In the past decade, various hydrogel-based, in vitro tumor models have been developed to mimic and recapitulate aspects of the tumor microenvironment in PDAC. Advances in hydrogel chemistry and engineering should provide a venue for discovering new insights regarding how matrix properties govern PDAC cell growth, migration, invasion, and drug resistance. These engineered hydrogels are ideal for understanding how variation in matrix properties contributes to the progressiveness of cancer cells, including durotaxis, the directional migration of cells in response to a stiffness gradient. This review surveys the various hydrogel-based, in vitro tumor models and the methods to generate gradient stiffness for studying migration and other cancer cell fate processes in PDAC. MDPI 2021-03-13 /pmc/articles/PMC8002168/ /pubmed/33805737 http://dx.doi.org/10.3390/bioengineering8030037 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Review
Chang, Chun-Yi
Lin, Chien-Chi
Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title_full Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title_fullStr Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title_full_unstemmed Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title_short Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate
title_sort hydrogel models with stiffness gradients for interrogating pancreatic cancer cell fate
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002168/
https://www.ncbi.nlm.nih.gov/pubmed/33805737
http://dx.doi.org/10.3390/bioengineering8030037
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