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PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System
Macrophages possess an innate ability to scavenge heterogenous objects from the systemic circulation and to regulate inflammatory diseases in various organs via cytokine production. That makes them attractive targets for nanomedicine-based therapeutic approaches to inflammatory diseases. In the pres...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002218/ https://www.ncbi.nlm.nih.gov/pubmed/33809764 http://dx.doi.org/10.3390/nano11030749 |
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author | Boltnarova, Barbora Kubackova, Jana Skoda, Josef Stefela, Alzbeta Smekalova, Monika Svacinova, Petra Pavkova, Ivona Dittrich, Milan Scherman, Daniel Zbytovska, Jarmila Pavek, Petr Holas, Ondrej |
author_facet | Boltnarova, Barbora Kubackova, Jana Skoda, Josef Stefela, Alzbeta Smekalova, Monika Svacinova, Petra Pavkova, Ivona Dittrich, Milan Scherman, Daniel Zbytovska, Jarmila Pavek, Petr Holas, Ondrej |
author_sort | Boltnarova, Barbora |
collection | PubMed |
description | Macrophages possess an innate ability to scavenge heterogenous objects from the systemic circulation and to regulate inflammatory diseases in various organs via cytokine production. That makes them attractive targets for nanomedicine-based therapeutic approaches to inflammatory diseases. In the present study, we have prepared several different poly(lactic-co-glycolic acid) (PLGA) polymer nanospheres for macrophage-targeted drug delivery using both nanoprecipitation and emulsification solvent evaporation methods. Two experimental linear PLGA polymers with relatively low molar weight, one experimental branched PLGA with unique star-like molecular architecture, and a commercially available PLGA, were used for nanosphere formulation and compared to their macrophage uptake capacity. The nanosphere formulations labelled with loaded fluorescent dye Rhodamine B were further tested in mouse bone marrow-derived macrophages and in hepatocyte cell lines AML-12, HepG2. We found that nanospheres larger than 100 nm prepared using nanoprecipitation significantly enhanced distribution of fluorescent dye selectively into macrophages. No effects of nanospheres on cellular viability were observed. Additionally, no significant proinflammatory effect after macrophage exposure to nanospheres was detected as assessed by a determination of proinflammatory cytokines Il-1β and Tnfα mRNA. All experimental PLGA nanoformulations surpassed the nanospheres obtained with the commercially available polymer taken as a control in their capacity as macrophage-specific carriers. |
format | Online Article Text |
id | pubmed-8002218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80022182021-03-28 PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System Boltnarova, Barbora Kubackova, Jana Skoda, Josef Stefela, Alzbeta Smekalova, Monika Svacinova, Petra Pavkova, Ivona Dittrich, Milan Scherman, Daniel Zbytovska, Jarmila Pavek, Petr Holas, Ondrej Nanomaterials (Basel) Article Macrophages possess an innate ability to scavenge heterogenous objects from the systemic circulation and to regulate inflammatory diseases in various organs via cytokine production. That makes them attractive targets for nanomedicine-based therapeutic approaches to inflammatory diseases. In the present study, we have prepared several different poly(lactic-co-glycolic acid) (PLGA) polymer nanospheres for macrophage-targeted drug delivery using both nanoprecipitation and emulsification solvent evaporation methods. Two experimental linear PLGA polymers with relatively low molar weight, one experimental branched PLGA with unique star-like molecular architecture, and a commercially available PLGA, were used for nanosphere formulation and compared to their macrophage uptake capacity. The nanosphere formulations labelled with loaded fluorescent dye Rhodamine B were further tested in mouse bone marrow-derived macrophages and in hepatocyte cell lines AML-12, HepG2. We found that nanospheres larger than 100 nm prepared using nanoprecipitation significantly enhanced distribution of fluorescent dye selectively into macrophages. No effects of nanospheres on cellular viability were observed. Additionally, no significant proinflammatory effect after macrophage exposure to nanospheres was detected as assessed by a determination of proinflammatory cytokines Il-1β and Tnfα mRNA. All experimental PLGA nanoformulations surpassed the nanospheres obtained with the commercially available polymer taken as a control in their capacity as macrophage-specific carriers. MDPI 2021-03-16 /pmc/articles/PMC8002218/ /pubmed/33809764 http://dx.doi.org/10.3390/nano11030749 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Boltnarova, Barbora Kubackova, Jana Skoda, Josef Stefela, Alzbeta Smekalova, Monika Svacinova, Petra Pavkova, Ivona Dittrich, Milan Scherman, Daniel Zbytovska, Jarmila Pavek, Petr Holas, Ondrej PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title | PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title_full | PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title_fullStr | PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title_full_unstemmed | PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title_short | PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System |
title_sort | plga based nanospheres as a potent macrophage-specific drug delivery system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002218/ https://www.ncbi.nlm.nih.gov/pubmed/33809764 http://dx.doi.org/10.3390/nano11030749 |
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