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Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels
Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002256/ https://www.ncbi.nlm.nih.gov/pubmed/33809738 http://dx.doi.org/10.3390/pathogens10030351 |
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author | Mohamed, Wessam Mohamed Ahmed Ali, Alsagher O. Mahmoud, Hassan Y. A. H. Omar, Mosaab A. Chatanga, Elisha Salim, Bashir Naguib, Doaa Anders, Jason L. Nonaka, Nariaki Moustafa, Mohamed Abdallah Mohamed Nakao, Ryo |
author_facet | Mohamed, Wessam Mohamed Ahmed Ali, Alsagher O. Mahmoud, Hassan Y. A. H. Omar, Mosaab A. Chatanga, Elisha Salim, Bashir Naguib, Doaa Anders, Jason L. Nonaka, Nariaki Moustafa, Mohamed Abdallah Mohamed Nakao, Ryo |
author_sort | Mohamed, Wessam Mohamed Ahmed |
collection | PubMed |
description | Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals. |
format | Online Article Text |
id | pubmed-8002256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80022562021-03-28 Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels Mohamed, Wessam Mohamed Ahmed Ali, Alsagher O. Mahmoud, Hassan Y. A. H. Omar, Mosaab A. Chatanga, Elisha Salim, Bashir Naguib, Doaa Anders, Jason L. Nonaka, Nariaki Moustafa, Mohamed Abdallah Mohamed Nakao, Ryo Pathogens Article Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals. MDPI 2021-03-16 /pmc/articles/PMC8002256/ /pubmed/33809738 http://dx.doi.org/10.3390/pathogens10030351 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Mohamed, Wessam Mohamed Ahmed Ali, Alsagher O. Mahmoud, Hassan Y. A. H. Omar, Mosaab A. Chatanga, Elisha Salim, Bashir Naguib, Doaa Anders, Jason L. Nonaka, Nariaki Moustafa, Mohamed Abdallah Mohamed Nakao, Ryo Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title_full | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title_fullStr | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title_full_unstemmed | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title_short | Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels |
title_sort | exploring prokaryotic and eukaryotic microbiomes helps in detecting tick-borne infectious agents in the blood of camels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002256/ https://www.ncbi.nlm.nih.gov/pubmed/33809738 http://dx.doi.org/10.3390/pathogens10030351 |
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