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Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery

Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term chan...

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Autores principales: Schmitt, Felix C. F., Schneider, Martin, Mathejczyk, William, Weigand, Markus A., Figueiredo, Jane C., Li, Christopher I., Shibata, David, Siegel, Erin M., Toriola, Adetunji T., Ulrich, Cornelia M., Ulrich, Alexis B., Boutin, Sébastien, Gigic, Biljana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002283/
https://www.ncbi.nlm.nih.gov/pubmed/33809741
http://dx.doi.org/10.3390/life11030246
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author Schmitt, Felix C. F.
Schneider, Martin
Mathejczyk, William
Weigand, Markus A.
Figueiredo, Jane C.
Li, Christopher I.
Shibata, David
Siegel, Erin M.
Toriola, Adetunji T.
Ulrich, Cornelia M.
Ulrich, Alexis B.
Boutin, Sébastien
Gigic, Biljana
author_facet Schmitt, Felix C. F.
Schneider, Martin
Mathejczyk, William
Weigand, Markus A.
Figueiredo, Jane C.
Li, Christopher I.
Shibata, David
Siegel, Erin M.
Toriola, Adetunji T.
Ulrich, Cornelia M.
Ulrich, Alexis B.
Boutin, Sébastien
Gigic, Biljana
author_sort Schmitt, Felix C. F.
collection PubMed
description Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups—patients who suffered from postoperative complications (n = 30) and those without complication (n = 32). Patients with postoperative complications showed a significantly stronger reduction in the alpha diversity starting 6 months after operation, which does not resolve, even after 24 months. The structure of the microbiome was also significantly altered from baseline at six-month follow-up in patients with complications (p = 0.006). This was associated with a long-lasting decrease of a large number of species in the gut microbiota indicating an impact in the commensal microbiota and a long-lasting increase of Fusobacterium ulcerans. The microbial composition of the gut microbiome shows significant changes in patients with postoperative complications up to 24 months after surgery.
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spelling pubmed-80022832021-03-28 Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery Schmitt, Felix C. F. Schneider, Martin Mathejczyk, William Weigand, Markus A. Figueiredo, Jane C. Li, Christopher I. Shibata, David Siegel, Erin M. Toriola, Adetunji T. Ulrich, Cornelia M. Ulrich, Alexis B. Boutin, Sébastien Gigic, Biljana Life (Basel) Article Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups—patients who suffered from postoperative complications (n = 30) and those without complication (n = 32). Patients with postoperative complications showed a significantly stronger reduction in the alpha diversity starting 6 months after operation, which does not resolve, even after 24 months. The structure of the microbiome was also significantly altered from baseline at six-month follow-up in patients with complications (p = 0.006). This was associated with a long-lasting decrease of a large number of species in the gut microbiota indicating an impact in the commensal microbiota and a long-lasting increase of Fusobacterium ulcerans. The microbial composition of the gut microbiome shows significant changes in patients with postoperative complications up to 24 months after surgery. MDPI 2021-03-16 /pmc/articles/PMC8002283/ /pubmed/33809741 http://dx.doi.org/10.3390/life11030246 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Schmitt, Felix C. F.
Schneider, Martin
Mathejczyk, William
Weigand, Markus A.
Figueiredo, Jane C.
Li, Christopher I.
Shibata, David
Siegel, Erin M.
Toriola, Adetunji T.
Ulrich, Cornelia M.
Ulrich, Alexis B.
Boutin, Sébastien
Gigic, Biljana
Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title_full Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title_fullStr Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title_full_unstemmed Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title_short Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
title_sort postoperative complications are associated with long-term changes in the gut microbiota following colorectal cancer surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002283/
https://www.ncbi.nlm.nih.gov/pubmed/33809741
http://dx.doi.org/10.3390/life11030246
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