Cargando…

Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications

One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber–polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize th...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghorbanzadeh Sheish, Shahnaz, Emadi, Rahmatollah, Ahmadian, Mehdi, Sadeghzade, Sorour, Tavangarian, Fariborz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002296/
https://www.ncbi.nlm.nih.gov/pubmed/33809630
http://dx.doi.org/10.3390/polym13060913
_version_ 1783671430496387072
author Ghorbanzadeh Sheish, Shahnaz
Emadi, Rahmatollah
Ahmadian, Mehdi
Sadeghzade, Sorour
Tavangarian, Fariborz
author_facet Ghorbanzadeh Sheish, Shahnaz
Emadi, Rahmatollah
Ahmadian, Mehdi
Sadeghzade, Sorour
Tavangarian, Fariborz
author_sort Ghorbanzadeh Sheish, Shahnaz
collection PubMed
description One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber–polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize the polyvinylpyrrolidone-acrylic acid hydrogel (PVPA)–eggshell membrane (ESM)–reduced graphene oxide (rGO) nanosheets nanocomposite dressings with different reduced graphene oxide contents (0, 0.5, 1, and 2 wt.%). Thus, smooth nanofibers were fabricated, including a high amount of rGO, which reduced the fiber diameter. Based on the results, rGO played an important role in water impermeability. The results showed that by increasing the rGO concentration from 0.5 to 2 wt%, the contact angle value increased persistently. Results showed that compared to PVPA–ESM, the mechanical strength and strain of PVPA–ESM/1 wt% rGO significantly enhanced 28% and 23%, respectively. Incorporation of 1 wt% rGO enhanced swelling ratio from 875% for PVPA-ESM to 1235% after 420 min, while increasing the rGO to 2 wt% increased the degradation rate of the composites. According to the in vitro cell culture studies, PVPA-ESM wound dressings with 0.5–1 wt% rGO content enhanced PC12 cell viability compared to the wound dressings without rGO nanosheets. Generally, rGO–loaded PVPA-ESM nanofiber wound dressing can be considered as a potential candidate to be used in skin regeneration applications.
format Online
Article
Text
id pubmed-8002296
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80022962021-03-28 Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications Ghorbanzadeh Sheish, Shahnaz Emadi, Rahmatollah Ahmadian, Mehdi Sadeghzade, Sorour Tavangarian, Fariborz Polymers (Basel) Article One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber–polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize the polyvinylpyrrolidone-acrylic acid hydrogel (PVPA)–eggshell membrane (ESM)–reduced graphene oxide (rGO) nanosheets nanocomposite dressings with different reduced graphene oxide contents (0, 0.5, 1, and 2 wt.%). Thus, smooth nanofibers were fabricated, including a high amount of rGO, which reduced the fiber diameter. Based on the results, rGO played an important role in water impermeability. The results showed that by increasing the rGO concentration from 0.5 to 2 wt%, the contact angle value increased persistently. Results showed that compared to PVPA–ESM, the mechanical strength and strain of PVPA–ESM/1 wt% rGO significantly enhanced 28% and 23%, respectively. Incorporation of 1 wt% rGO enhanced swelling ratio from 875% for PVPA-ESM to 1235% after 420 min, while increasing the rGO to 2 wt% increased the degradation rate of the composites. According to the in vitro cell culture studies, PVPA-ESM wound dressings with 0.5–1 wt% rGO content enhanced PC12 cell viability compared to the wound dressings without rGO nanosheets. Generally, rGO–loaded PVPA-ESM nanofiber wound dressing can be considered as a potential candidate to be used in skin regeneration applications. MDPI 2021-03-16 /pmc/articles/PMC8002296/ /pubmed/33809630 http://dx.doi.org/10.3390/polym13060913 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghorbanzadeh Sheish, Shahnaz
Emadi, Rahmatollah
Ahmadian, Mehdi
Sadeghzade, Sorour
Tavangarian, Fariborz
Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title_full Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title_fullStr Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title_full_unstemmed Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title_short Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications
title_sort fabrication and characterization of polyvinylpyrrolidone-eggshell membrane-reduced graphene oxide nanofibers for tissue engineering applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002296/
https://www.ncbi.nlm.nih.gov/pubmed/33809630
http://dx.doi.org/10.3390/polym13060913
work_keys_str_mv AT ghorbanzadehsheishshahnaz fabricationandcharacterizationofpolyvinylpyrrolidoneeggshellmembranereducedgrapheneoxidenanofibersfortissueengineeringapplications
AT emadirahmatollah fabricationandcharacterizationofpolyvinylpyrrolidoneeggshellmembranereducedgrapheneoxidenanofibersfortissueengineeringapplications
AT ahmadianmehdi fabricationandcharacterizationofpolyvinylpyrrolidoneeggshellmembranereducedgrapheneoxidenanofibersfortissueengineeringapplications
AT sadeghzadesorour fabricationandcharacterizationofpolyvinylpyrrolidoneeggshellmembranereducedgrapheneoxidenanofibersfortissueengineeringapplications
AT tavangarianfariborz fabricationandcharacterizationofpolyvinylpyrrolidoneeggshellmembranereducedgrapheneoxidenanofibersfortissueengineeringapplications