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Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation

Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stre...

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Autores principales: Tadić, Vanja M., Nešić, Ivana, Martinović, Milica, Rój, Edward, Brašanac-Vukanović, Snežana, Maksimović, Svetolik, Žugić, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002374/
https://www.ncbi.nlm.nih.gov/pubmed/33809607
http://dx.doi.org/10.3390/antiox10030465
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author Tadić, Vanja M.
Nešić, Ivana
Martinović, Milica
Rój, Edward
Brašanac-Vukanović, Snežana
Maksimović, Svetolik
Žugić, Ana
author_facet Tadić, Vanja M.
Nešić, Ivana
Martinović, Milica
Rój, Edward
Brašanac-Vukanović, Snežana
Maksimović, Svetolik
Žugić, Ana
author_sort Tadić, Vanja M.
collection PubMed
description Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stress might cause skin impairments, we aim to evaluate a topical preparation containing bilberry leaves extract and bilberry seeds oil, obtained as a byproduct of the food industry. To obtain the extracts, the conventional maceration technique for leaves, and supercritical carbon dioxide extraction for seeds were employed. The chemical profile of both actives was achieved by HPLC and GC methods, revealing the presence of phenolic acids (chlorogenic being the most abundant), flavonoids (isoquercetin in the highest amount), and resveratrol in leaves extract, while in seeds oil the essential ω-3 and ω-6 fatty acids were determined in favorable ratio, almost being 1. Antioxidant potential of the wild bilberry extract and seed oil was evaluated using in vitro DPPH and FRAP assays. Finally, effects of the oil-in-water creams with mentioned wild bilberry isolates on the skin were investigated in an in vivo study conducted on healthy human volunteers, revealing the significant beneficial effects when topically applied.
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spelling pubmed-80023742021-03-28 Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation Tadić, Vanja M. Nešić, Ivana Martinović, Milica Rój, Edward Brašanac-Vukanović, Snežana Maksimović, Svetolik Žugić, Ana Antioxidants (Basel) Article Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stress might cause skin impairments, we aim to evaluate a topical preparation containing bilberry leaves extract and bilberry seeds oil, obtained as a byproduct of the food industry. To obtain the extracts, the conventional maceration technique for leaves, and supercritical carbon dioxide extraction for seeds were employed. The chemical profile of both actives was achieved by HPLC and GC methods, revealing the presence of phenolic acids (chlorogenic being the most abundant), flavonoids (isoquercetin in the highest amount), and resveratrol in leaves extract, while in seeds oil the essential ω-3 and ω-6 fatty acids were determined in favorable ratio, almost being 1. Antioxidant potential of the wild bilberry extract and seed oil was evaluated using in vitro DPPH and FRAP assays. Finally, effects of the oil-in-water creams with mentioned wild bilberry isolates on the skin were investigated in an in vivo study conducted on healthy human volunteers, revealing the significant beneficial effects when topically applied. MDPI 2021-03-16 /pmc/articles/PMC8002374/ /pubmed/33809607 http://dx.doi.org/10.3390/antiox10030465 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Tadić, Vanja M.
Nešić, Ivana
Martinović, Milica
Rój, Edward
Brašanac-Vukanović, Snežana
Maksimović, Svetolik
Žugić, Ana
Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title_full Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title_fullStr Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title_full_unstemmed Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title_short Old Plant, New Possibilities: Wild Bilberry (Vaccinium myrtillus L., Ericaceae) in Topical Skin Preparation
title_sort old plant, new possibilities: wild bilberry (vaccinium myrtillus l., ericaceae) in topical skin preparation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002374/
https://www.ncbi.nlm.nih.gov/pubmed/33809607
http://dx.doi.org/10.3390/antiox10030465
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