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Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4
Hepatitis B virus (HBV) classification comprises up to 10 genotypes with specific geographical distribution worldwide, further subdivided into 40 subgenotypes, which have different impacts on liver disease outcome. Though extensively studied, the classification of subgenotype A sequences remains amb...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002614/ https://www.ncbi.nlm.nih.gov/pubmed/33803011 http://dx.doi.org/10.3390/microorganisms9030623 |
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author | Toyé, Rayana Maryse Cohen, Damien Pujol, Flor Helene Sow-Sall, Amina Lô, Gora Hoshino, Kunikazu Mizokami, Masashi Zoulim, Fabien Lemoine, Maud Touré-Kane, Coumba Chemin, Isabelle |
author_facet | Toyé, Rayana Maryse Cohen, Damien Pujol, Flor Helene Sow-Sall, Amina Lô, Gora Hoshino, Kunikazu Mizokami, Masashi Zoulim, Fabien Lemoine, Maud Touré-Kane, Coumba Chemin, Isabelle |
author_sort | Toyé, Rayana Maryse |
collection | PubMed |
description | Hepatitis B virus (HBV) classification comprises up to 10 genotypes with specific geographical distribution worldwide, further subdivided into 40 subgenotypes, which have different impacts on liver disease outcome. Though extensively studied, the classification of subgenotype A sequences remains ambiguous. This study aimed to characterize HBV isolates from West African patients and propose a more advanced classification of subgenotype A. Fourteen HBV full-length genome sequences isolated from patients from The Gambia and Senegal were obtained and phylogenetically analyzed. Phylogenetic analysis of HBV genotype A sequences isolated from Senegalese and Gambian patients exhibited separate clusters from the other known and confirmed subgenotypes A (A1, A2, A6). Most of the sequences (10/14) clustered with an isolate from Cuba, reported as subgenotype A4 (supported by maximal bootstrap value). Four isolates from The Gambia and Senegal clustered separately from all other subgenotypes and samples sequenced in the study. Three of which from The Gambia, designated as an expanding clade of subgenotype A4, exhibited a mean inter-subgenotypic nucleotide divergence over the entire genome sequence higher than 4% in comparison with the other subgenotypes and the other isolates sequenced in the study, except with subgenotype A4 isolates (3.9%), and this was supported by a maximal bootstrap value. The last one from Senegal seemed to be an expanding subgenotype close to the new clade of A4. Amino acid analysis unveiled a novel motif specific to these isolates. This study revealed an expanding evolution of HBV subgenotype A and novel amino acid motifs. It also highlighted the need for a consensus regarding the analysis and classification of HBV sequences. |
format | Online Article Text |
id | pubmed-8002614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80026142021-03-28 Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 Toyé, Rayana Maryse Cohen, Damien Pujol, Flor Helene Sow-Sall, Amina Lô, Gora Hoshino, Kunikazu Mizokami, Masashi Zoulim, Fabien Lemoine, Maud Touré-Kane, Coumba Chemin, Isabelle Microorganisms Communication Hepatitis B virus (HBV) classification comprises up to 10 genotypes with specific geographical distribution worldwide, further subdivided into 40 subgenotypes, which have different impacts on liver disease outcome. Though extensively studied, the classification of subgenotype A sequences remains ambiguous. This study aimed to characterize HBV isolates from West African patients and propose a more advanced classification of subgenotype A. Fourteen HBV full-length genome sequences isolated from patients from The Gambia and Senegal were obtained and phylogenetically analyzed. Phylogenetic analysis of HBV genotype A sequences isolated from Senegalese and Gambian patients exhibited separate clusters from the other known and confirmed subgenotypes A (A1, A2, A6). Most of the sequences (10/14) clustered with an isolate from Cuba, reported as subgenotype A4 (supported by maximal bootstrap value). Four isolates from The Gambia and Senegal clustered separately from all other subgenotypes and samples sequenced in the study. Three of which from The Gambia, designated as an expanding clade of subgenotype A4, exhibited a mean inter-subgenotypic nucleotide divergence over the entire genome sequence higher than 4% in comparison with the other subgenotypes and the other isolates sequenced in the study, except with subgenotype A4 isolates (3.9%), and this was supported by a maximal bootstrap value. The last one from Senegal seemed to be an expanding subgenotype close to the new clade of A4. Amino acid analysis unveiled a novel motif specific to these isolates. This study revealed an expanding evolution of HBV subgenotype A and novel amino acid motifs. It also highlighted the need for a consensus regarding the analysis and classification of HBV sequences. MDPI 2021-03-17 /pmc/articles/PMC8002614/ /pubmed/33803011 http://dx.doi.org/10.3390/microorganisms9030623 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Communication Toyé, Rayana Maryse Cohen, Damien Pujol, Flor Helene Sow-Sall, Amina Lô, Gora Hoshino, Kunikazu Mizokami, Masashi Zoulim, Fabien Lemoine, Maud Touré-Kane, Coumba Chemin, Isabelle Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title | Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title_full | Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title_fullStr | Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title_full_unstemmed | Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title_short | Hepatitis B Virus Genotype Study in West Africa Reveals an Expanding Clade of Subgenotype A4 |
title_sort | hepatitis b virus genotype study in west africa reveals an expanding clade of subgenotype a4 |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002614/ https://www.ncbi.nlm.nih.gov/pubmed/33803011 http://dx.doi.org/10.3390/microorganisms9030623 |
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