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Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines

Universal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides...

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Autores principales: Salcines-Cuevas, David, Terán-Navarro, Hector, Calderón-Gonzalez, Ricardo, Torres-Rodriguez, Paula, Tobes, Raquel, Fresno, Manuel, Calvo-Montes, Jorge, Molino-Bernal, I. Concepción Pérez Del, Yañez-Diaz, Sonsoles, Alvarez-Dominguez, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002646/
https://www.ncbi.nlm.nih.gov/pubmed/33802959
http://dx.doi.org/10.3390/vaccines9030269
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author Salcines-Cuevas, David
Terán-Navarro, Hector
Calderón-Gonzalez, Ricardo
Torres-Rodriguez, Paula
Tobes, Raquel
Fresno, Manuel
Calvo-Montes, Jorge
Molino-Bernal, I. Concepción Pérez Del
Yañez-Diaz, Sonsoles
Alvarez-Dominguez, Carmen
author_facet Salcines-Cuevas, David
Terán-Navarro, Hector
Calderón-Gonzalez, Ricardo
Torres-Rodriguez, Paula
Tobes, Raquel
Fresno, Manuel
Calvo-Montes, Jorge
Molino-Bernal, I. Concepción Pérez Del
Yañez-Diaz, Sonsoles
Alvarez-Dominguez, Carmen
author_sort Salcines-Cuevas, David
collection PubMed
description Universal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides, GAPDH-L1 (Listeria), GAPDH-M1 (Mycobacterium) and GAPDH-S1 (Streptococcus), share 95–98.55% sequence homology, biochemical and MHC binding abilities and, therefore, are good candidates for universal vaccine designs. Here, we used dendritic cells (DC) as vaccine platforms to test GAPDH epitopes that conferred protection against Listeria monocytogenes, Mycobacterium marinum or Streptococcus pneumoniae in our search of epitopes for universal vaccines. DC loaded with GAPDH-L1, GAPDH-M1 or GAPDH-S1 peptides show high immunogenicity measured by the cellular DTH responses in mice, lacked toxicity and were capable of cross-protection immunity against mice infections with each one of the pathogens. Vaccine efficiency correlated with high titers of anti-GAPDH-L1 antibodies in sera of vaccinated mice, a Th1 cytokine pattern and high frequencies of GAPDH-L1-specific CD4+ and CD8+ T cells and IFN-γ producers in the spleens. We concluded that GAPDH-L1 peptide was the best epitope for universal vaccines in the Listeria, Mycobacterium or Streptococcus taxonomic groups, whose pathogenic strains caused relevant morbidities in adults and especially in the elderly.
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spelling pubmed-80026462021-03-28 Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines Salcines-Cuevas, David Terán-Navarro, Hector Calderón-Gonzalez, Ricardo Torres-Rodriguez, Paula Tobes, Raquel Fresno, Manuel Calvo-Montes, Jorge Molino-Bernal, I. Concepción Pérez Del Yañez-Diaz, Sonsoles Alvarez-Dominguez, Carmen Vaccines (Basel) Article Universal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides, GAPDH-L1 (Listeria), GAPDH-M1 (Mycobacterium) and GAPDH-S1 (Streptococcus), share 95–98.55% sequence homology, biochemical and MHC binding abilities and, therefore, are good candidates for universal vaccine designs. Here, we used dendritic cells (DC) as vaccine platforms to test GAPDH epitopes that conferred protection against Listeria monocytogenes, Mycobacterium marinum or Streptococcus pneumoniae in our search of epitopes for universal vaccines. DC loaded with GAPDH-L1, GAPDH-M1 or GAPDH-S1 peptides show high immunogenicity measured by the cellular DTH responses in mice, lacked toxicity and were capable of cross-protection immunity against mice infections with each one of the pathogens. Vaccine efficiency correlated with high titers of anti-GAPDH-L1 antibodies in sera of vaccinated mice, a Th1 cytokine pattern and high frequencies of GAPDH-L1-specific CD4+ and CD8+ T cells and IFN-γ producers in the spleens. We concluded that GAPDH-L1 peptide was the best epitope for universal vaccines in the Listeria, Mycobacterium or Streptococcus taxonomic groups, whose pathogenic strains caused relevant morbidities in adults and especially in the elderly. MDPI 2021-03-17 /pmc/articles/PMC8002646/ /pubmed/33802959 http://dx.doi.org/10.3390/vaccines9030269 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Salcines-Cuevas, David
Terán-Navarro, Hector
Calderón-Gonzalez, Ricardo
Torres-Rodriguez, Paula
Tobes, Raquel
Fresno, Manuel
Calvo-Montes, Jorge
Molino-Bernal, I. Concepción Pérez Del
Yañez-Diaz, Sonsoles
Alvarez-Dominguez, Carmen
Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title_full Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title_fullStr Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title_full_unstemmed Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title_short Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines
title_sort glyceraldehyde-3-phosphate dehydrogenase common peptides of listeria monocytogenes, mycobacterium marinum and streptococcus pneumoniae as universal vaccines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002646/
https://www.ncbi.nlm.nih.gov/pubmed/33802959
http://dx.doi.org/10.3390/vaccines9030269
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