Cargando…

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells

Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR...

Descripción completa

Detalles Bibliográficos
Autores principales: Tirpáková, Mária, Vašíček, Jaromír, Svoradová, Andrea, Baláži, Andrej, Tomka, Marián, Bauer, Miroslav, Makarevich, Alexander, Chrenek, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002684/
https://www.ncbi.nlm.nih.gov/pubmed/33802902
http://dx.doi.org/10.3390/genes12030431
_version_ 1783671521115373568
author Tirpáková, Mária
Vašíček, Jaromír
Svoradová, Andrea
Baláži, Andrej
Tomka, Marián
Bauer, Miroslav
Makarevich, Alexander
Chrenek, Peter
author_facet Tirpáková, Mária
Vašíček, Jaromír
Svoradová, Andrea
Baláži, Andrej
Tomka, Marián
Bauer, Miroslav
Makarevich, Alexander
Chrenek, Peter
author_sort Tirpáková, Mária
collection PubMed
description Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank.
format Online
Article
Text
id pubmed-8002684
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80026842021-03-28 Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells Tirpáková, Mária Vašíček, Jaromír Svoradová, Andrea Baláži, Andrej Tomka, Marián Bauer, Miroslav Makarevich, Alexander Chrenek, Peter Genes (Basel) Article Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank. MDPI 2021-03-17 /pmc/articles/PMC8002684/ /pubmed/33802902 http://dx.doi.org/10.3390/genes12030431 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Tirpáková, Mária
Vašíček, Jaromír
Svoradová, Andrea
Baláži, Andrej
Tomka, Marián
Bauer, Miroslav
Makarevich, Alexander
Chrenek, Peter
Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title_full Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title_fullStr Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title_full_unstemmed Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title_short Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
title_sort phenotypical characterization and neurogenic differentiation of rabbit adipose tissue-derived mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002684/
https://www.ncbi.nlm.nih.gov/pubmed/33802902
http://dx.doi.org/10.3390/genes12030431
work_keys_str_mv AT tirpakovamaria phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT vasicekjaromir phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT svoradovaandrea phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT balaziandrej phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT tomkamarian phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT bauermiroslav phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT makarevichalexander phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells
AT chrenekpeter phenotypicalcharacterizationandneurogenicdifferentiationofrabbitadiposetissuederivedmesenchymalstemcells