Cargando…
Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells
The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxi...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002759/ https://www.ncbi.nlm.nih.gov/pubmed/33802935 http://dx.doi.org/10.3390/antiox10030476 |
_version_ | 1783671538580455424 |
---|---|
author | Lee, Da-Yeon Chun, Yoon-Seok Kim, Jong-Kyu Lee, Jeong-Ok Lee, Young-Joon Ku, Sae-Kwang Shim, Soon-Mi |
author_facet | Lee, Da-Yeon Chun, Yoon-Seok Kim, Jong-Kyu Lee, Jeong-Ok Lee, Young-Joon Ku, Sae-Kwang Shim, Soon-Mi |
author_sort | Lee, Da-Yeon |
collection | PubMed |
description | The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxicity in C2C12 cells. Exposure to hydrogen peroxide (H(2)O(2)) drastically decreased the viability of C2C12 cells, but pre-treatment of CM-SD significantly increased the cell viability (p < 0.01). CM-SD significantly transactivated the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent luciferase activity in a dose-dependent manner and enhanced the levels of heme oxygenase (HO)-1, glutamate cysteine ligase catalytic subunit (GCLC), and NAD(P)H-dependent quinone oxidoreductase (NQO)-1. CM-SD also significantly reduced reactive oxygen species (ROS) production and lipid peroxidation and restored glutathione (GSH) depletion in H(2)O(2)-treated C2C12 cells. Moreover, CM-SD significantly reduced lipopolysaccharides (LPS)-mediated interleukin (IL)-6 production in the conditioned medium. Results from the current study suggest that CM-SD could be a useful candidate against oxidative stress and inflammation-related muscle disorders. |
format | Online Article Text |
id | pubmed-8002759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80027592021-03-28 Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells Lee, Da-Yeon Chun, Yoon-Seok Kim, Jong-Kyu Lee, Jeong-Ok Lee, Young-Joon Ku, Sae-Kwang Shim, Soon-Mi Antioxidants (Basel) Article The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxicity in C2C12 cells. Exposure to hydrogen peroxide (H(2)O(2)) drastically decreased the viability of C2C12 cells, but pre-treatment of CM-SD significantly increased the cell viability (p < 0.01). CM-SD significantly transactivated the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent luciferase activity in a dose-dependent manner and enhanced the levels of heme oxygenase (HO)-1, glutamate cysteine ligase catalytic subunit (GCLC), and NAD(P)H-dependent quinone oxidoreductase (NQO)-1. CM-SD also significantly reduced reactive oxygen species (ROS) production and lipid peroxidation and restored glutathione (GSH) depletion in H(2)O(2)-treated C2C12 cells. Moreover, CM-SD significantly reduced lipopolysaccharides (LPS)-mediated interleukin (IL)-6 production in the conditioned medium. Results from the current study suggest that CM-SD could be a useful candidate against oxidative stress and inflammation-related muscle disorders. MDPI 2021-03-17 /pmc/articles/PMC8002759/ /pubmed/33802935 http://dx.doi.org/10.3390/antiox10030476 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Lee, Da-Yeon Chun, Yoon-Seok Kim, Jong-Kyu Lee, Jeong-Ok Lee, Young-Joon Ku, Sae-Kwang Shim, Soon-Mi Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title | Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title_full | Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title_fullStr | Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title_full_unstemmed | Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title_short | Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells |
title_sort | curcumin ameliorated oxidative stress and inflammation-related muscle disorders in c2c12 myoblast cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002759/ https://www.ncbi.nlm.nih.gov/pubmed/33802935 http://dx.doi.org/10.3390/antiox10030476 |
work_keys_str_mv | AT leedayeon curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT chunyoonseok curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT kimjongkyu curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT leejeongok curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT leeyoungjoon curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT kusaekwang curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells AT shimsoonmi curcuminamelioratedoxidativestressandinflammationrelatedmuscledisordersinc2c12myoblastcells |