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Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies

Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release...

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Autores principales: Torelli, Alessandro, Gianchecchi, Elena, Monti, Martina, Piu, Pietro, Barneschi, Irene, Bonifazi, Carolina, Coluccio, Rosa, Ganfini, Luisa, Magra, Luciano Michele La, Marconi, Silvia, Marzucchi, Ginevra, Pace, Ramona, Palladino, Laura, Biagi, Bernardo, Montomoli, Emanuele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002830/
https://www.ncbi.nlm.nih.gov/pubmed/33802846
http://dx.doi.org/10.3390/vaccines9030267
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author Torelli, Alessandro
Gianchecchi, Elena
Monti, Martina
Piu, Pietro
Barneschi, Irene
Bonifazi, Carolina
Coluccio, Rosa
Ganfini, Luisa
Magra, Luciano Michele La
Marconi, Silvia
Marzucchi, Ginevra
Pace, Ramona
Palladino, Laura
Biagi, Bernardo
Montomoli, Emanuele
author_facet Torelli, Alessandro
Gianchecchi, Elena
Monti, Martina
Piu, Pietro
Barneschi, Irene
Bonifazi, Carolina
Coluccio, Rosa
Ganfini, Luisa
Magra, Luciano Michele La
Marconi, Silvia
Marzucchi, Ginevra
Pace, Ramona
Palladino, Laura
Biagi, Bernardo
Montomoli, Emanuele
author_sort Torelli, Alessandro
collection PubMed
description Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles.
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spelling pubmed-80028302021-03-28 Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies Torelli, Alessandro Gianchecchi, Elena Monti, Martina Piu, Pietro Barneschi, Irene Bonifazi, Carolina Coluccio, Rosa Ganfini, Luisa Magra, Luciano Michele La Marconi, Silvia Marzucchi, Ginevra Pace, Ramona Palladino, Laura Biagi, Bernardo Montomoli, Emanuele Vaccines (Basel) Article Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles. MDPI 2021-03-17 /pmc/articles/PMC8002830/ /pubmed/33802846 http://dx.doi.org/10.3390/vaccines9030267 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Torelli, Alessandro
Gianchecchi, Elena
Monti, Martina
Piu, Pietro
Barneschi, Irene
Bonifazi, Carolina
Coluccio, Rosa
Ganfini, Luisa
Magra, Luciano Michele La
Marconi, Silvia
Marzucchi, Ginevra
Pace, Ramona
Palladino, Laura
Biagi, Bernardo
Montomoli, Emanuele
Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title_full Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title_fullStr Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title_full_unstemmed Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title_short Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
title_sort effect of repeated freeze–thaw cycles on influenza virus antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002830/
https://www.ncbi.nlm.nih.gov/pubmed/33802846
http://dx.doi.org/10.3390/vaccines9030267
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