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Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002830/ https://www.ncbi.nlm.nih.gov/pubmed/33802846 http://dx.doi.org/10.3390/vaccines9030267 |
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author | Torelli, Alessandro Gianchecchi, Elena Monti, Martina Piu, Pietro Barneschi, Irene Bonifazi, Carolina Coluccio, Rosa Ganfini, Luisa Magra, Luciano Michele La Marconi, Silvia Marzucchi, Ginevra Pace, Ramona Palladino, Laura Biagi, Bernardo Montomoli, Emanuele |
author_facet | Torelli, Alessandro Gianchecchi, Elena Monti, Martina Piu, Pietro Barneschi, Irene Bonifazi, Carolina Coluccio, Rosa Ganfini, Luisa Magra, Luciano Michele La Marconi, Silvia Marzucchi, Ginevra Pace, Ramona Palladino, Laura Biagi, Bernardo Montomoli, Emanuele |
author_sort | Torelli, Alessandro |
collection | PubMed |
description | Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles. |
format | Online Article Text |
id | pubmed-8002830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80028302021-03-28 Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies Torelli, Alessandro Gianchecchi, Elena Monti, Martina Piu, Pietro Barneschi, Irene Bonifazi, Carolina Coluccio, Rosa Ganfini, Luisa Magra, Luciano Michele La Marconi, Silvia Marzucchi, Ginevra Pace, Ramona Palladino, Laura Biagi, Bernardo Montomoli, Emanuele Vaccines (Basel) Article Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles. MDPI 2021-03-17 /pmc/articles/PMC8002830/ /pubmed/33802846 http://dx.doi.org/10.3390/vaccines9030267 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Torelli, Alessandro Gianchecchi, Elena Monti, Martina Piu, Pietro Barneschi, Irene Bonifazi, Carolina Coluccio, Rosa Ganfini, Luisa Magra, Luciano Michele La Marconi, Silvia Marzucchi, Ginevra Pace, Ramona Palladino, Laura Biagi, Bernardo Montomoli, Emanuele Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title | Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title_full | Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title_fullStr | Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title_full_unstemmed | Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title_short | Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies |
title_sort | effect of repeated freeze–thaw cycles on influenza virus antibodies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002830/ https://www.ncbi.nlm.nih.gov/pubmed/33802846 http://dx.doi.org/10.3390/vaccines9030267 |
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