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Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to...

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Autores principales: Kim, Hyung-Mo, Oh, Chiwoo, An, Jaehyun, Baek, Seungki, Bock, Sungje, Kim, Jaehi, Jung, Heung-Su, Song, Hobeom, Kim, Jung-Won, Jo, Ahla, Kim, Dong-Eun, Rho, Won-Yeop, Jang, Jin-Young, Cheon, Gi Jeong, Im, Hyung-Jun, Jun, Bong-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002883/
https://www.ncbi.nlm.nih.gov/pubmed/33803623
http://dx.doi.org/10.3390/nano11030768
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author Kim, Hyung-Mo
Oh, Chiwoo
An, Jaehyun
Baek, Seungki
Bock, Sungje
Kim, Jaehi
Jung, Heung-Su
Song, Hobeom
Kim, Jung-Won
Jo, Ahla
Kim, Dong-Eun
Rho, Won-Yeop
Jang, Jin-Young
Cheon, Gi Jeong
Im, Hyung-Jun
Jun, Bong-Hyun
author_facet Kim, Hyung-Mo
Oh, Chiwoo
An, Jaehyun
Baek, Seungki
Bock, Sungje
Kim, Jaehi
Jung, Heung-Su
Song, Hobeom
Kim, Jung-Won
Jo, Ahla
Kim, Dong-Eun
Rho, Won-Yeop
Jang, Jin-Young
Cheon, Gi Jeong
Im, Hyung-Jun
Jun, Bong-Hyun
author_sort Kim, Hyung-Mo
collection PubMed
description Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.
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spelling pubmed-80028832021-03-28 Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection Kim, Hyung-Mo Oh, Chiwoo An, Jaehyun Baek, Seungki Bock, Sungje Kim, Jaehi Jung, Heung-Su Song, Hobeom Kim, Jung-Won Jo, Ahla Kim, Dong-Eun Rho, Won-Yeop Jang, Jin-Young Cheon, Gi Jeong Im, Hyung-Jun Jun, Bong-Hyun Nanomaterials (Basel) Article Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs. MDPI 2021-03-18 /pmc/articles/PMC8002883/ /pubmed/33803623 http://dx.doi.org/10.3390/nano11030768 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Kim, Hyung-Mo
Oh, Chiwoo
An, Jaehyun
Baek, Seungki
Bock, Sungje
Kim, Jaehi
Jung, Heung-Su
Song, Hobeom
Kim, Jung-Won
Jo, Ahla
Kim, Dong-Eun
Rho, Won-Yeop
Jang, Jin-Young
Cheon, Gi Jeong
Im, Hyung-Jun
Jun, Bong-Hyun
Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title_full Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title_fullStr Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title_full_unstemmed Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title_short Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
title_sort multi-quantum dots-embedded silica-encapsulated nanoparticle-based lateral flow assay for highly sensitive exosome detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002883/
https://www.ncbi.nlm.nih.gov/pubmed/33803623
http://dx.doi.org/10.3390/nano11030768
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