Cargando…
Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection
Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002883/ https://www.ncbi.nlm.nih.gov/pubmed/33803623 http://dx.doi.org/10.3390/nano11030768 |
_version_ | 1783671558514933760 |
---|---|
author | Kim, Hyung-Mo Oh, Chiwoo An, Jaehyun Baek, Seungki Bock, Sungje Kim, Jaehi Jung, Heung-Su Song, Hobeom Kim, Jung-Won Jo, Ahla Kim, Dong-Eun Rho, Won-Yeop Jang, Jin-Young Cheon, Gi Jeong Im, Hyung-Jun Jun, Bong-Hyun |
author_facet | Kim, Hyung-Mo Oh, Chiwoo An, Jaehyun Baek, Seungki Bock, Sungje Kim, Jaehi Jung, Heung-Su Song, Hobeom Kim, Jung-Won Jo, Ahla Kim, Dong-Eun Rho, Won-Yeop Jang, Jin-Young Cheon, Gi Jeong Im, Hyung-Jun Jun, Bong-Hyun |
author_sort | Kim, Hyung-Mo |
collection | PubMed |
description | Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs. |
format | Online Article Text |
id | pubmed-8002883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80028832021-03-28 Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection Kim, Hyung-Mo Oh, Chiwoo An, Jaehyun Baek, Seungki Bock, Sungje Kim, Jaehi Jung, Heung-Su Song, Hobeom Kim, Jung-Won Jo, Ahla Kim, Dong-Eun Rho, Won-Yeop Jang, Jin-Young Cheon, Gi Jeong Im, Hyung-Jun Jun, Bong-Hyun Nanomaterials (Basel) Article Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs. MDPI 2021-03-18 /pmc/articles/PMC8002883/ /pubmed/33803623 http://dx.doi.org/10.3390/nano11030768 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Kim, Hyung-Mo Oh, Chiwoo An, Jaehyun Baek, Seungki Bock, Sungje Kim, Jaehi Jung, Heung-Su Song, Hobeom Kim, Jung-Won Jo, Ahla Kim, Dong-Eun Rho, Won-Yeop Jang, Jin-Young Cheon, Gi Jeong Im, Hyung-Jun Jun, Bong-Hyun Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title | Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title_full | Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title_fullStr | Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title_full_unstemmed | Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title_short | Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection |
title_sort | multi-quantum dots-embedded silica-encapsulated nanoparticle-based lateral flow assay for highly sensitive exosome detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002883/ https://www.ncbi.nlm.nih.gov/pubmed/33803623 http://dx.doi.org/10.3390/nano11030768 |
work_keys_str_mv | AT kimhyungmo multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT ohchiwoo multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT anjaehyun multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT baekseungki multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT bocksungje multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT kimjaehi multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT jungheungsu multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT songhobeom multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT kimjungwon multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT joahla multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT kimdongeun multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT rhowonyeop multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT jangjinyoung multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT cheongijeong multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT imhyungjun multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection AT junbonghyun multiquantumdotsembeddedsilicaencapsulatednanoparticlebasedlateralflowassayforhighlysensitiveexosomedetection |