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Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages

Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumat...

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Autores principales: Kim, Eui Jin, Seo, Ji Bin, Yu, Jae Sik, Lee, Seoyoung, Lim, Jae Sung, Choi, Jeong Uk, Lee, Chang-Min, Rashan, Luay, Kim, Ki Hyun, Cho, Young-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003360/
https://www.ncbi.nlm.nih.gov/pubmed/33808759
http://dx.doi.org/10.3390/pharmaceutics13030408
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author Kim, Eui Jin
Seo, Ji Bin
Yu, Jae Sik
Lee, Seoyoung
Lim, Jae Sung
Choi, Jeong Uk
Lee, Chang-Min
Rashan, Luay
Kim, Ki Hyun
Cho, Young-Chang
author_facet Kim, Eui Jin
Seo, Ji Bin
Yu, Jae Sik
Lee, Seoyoung
Lim, Jae Sung
Choi, Jeong Uk
Lee, Chang-Min
Rashan, Luay
Kim, Ki Hyun
Cho, Young-Chang
author_sort Kim, Eui Jin
collection PubMed
description Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumatoid arthritis. In continuation with our work related to the discovery of bioactive natural products, a polyphenol, catechin-7,4′-O-digallate (CDG), was isolated from Woodfordia uniflora, which has been used as a sedative and remedy for skin infections in the Dhofar region of Oman. Thus far, no study has reported the anti-inflammatory compounds derived from W. uniflora and the mechanisms underlying their action. To investigate the effects of CDG on the regulation of inflammation, we measured the reduction in nitric oxide (NO) production following CDG treatment in immortalized mouse Kupffer cells (ImKCs). CDG treatment inhibited NO production through the downregulation of inducible nitric oxide synthase expression in lipopolysaccharide (LPS)-stimulated ImKCs. The anti-inflammatory effects of CDG were mediated via the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, an important inflammatory-response-associated signaling pathway. Moreover, CDG treatment has regulated the expression of pro-inflammatory cytokines, such as IL-6 and IL-1β. These results suggested the anti-inflammatory action of CDG in LPS-stimulated ImKCs.
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spelling pubmed-80033602021-03-28 Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages Kim, Eui Jin Seo, Ji Bin Yu, Jae Sik Lee, Seoyoung Lim, Jae Sung Choi, Jeong Uk Lee, Chang-Min Rashan, Luay Kim, Ki Hyun Cho, Young-Chang Pharmaceutics Article Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumatoid arthritis. In continuation with our work related to the discovery of bioactive natural products, a polyphenol, catechin-7,4′-O-digallate (CDG), was isolated from Woodfordia uniflora, which has been used as a sedative and remedy for skin infections in the Dhofar region of Oman. Thus far, no study has reported the anti-inflammatory compounds derived from W. uniflora and the mechanisms underlying their action. To investigate the effects of CDG on the regulation of inflammation, we measured the reduction in nitric oxide (NO) production following CDG treatment in immortalized mouse Kupffer cells (ImKCs). CDG treatment inhibited NO production through the downregulation of inducible nitric oxide synthase expression in lipopolysaccharide (LPS)-stimulated ImKCs. The anti-inflammatory effects of CDG were mediated via the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, an important inflammatory-response-associated signaling pathway. Moreover, CDG treatment has regulated the expression of pro-inflammatory cytokines, such as IL-6 and IL-1β. These results suggested the anti-inflammatory action of CDG in LPS-stimulated ImKCs. MDPI 2021-03-19 /pmc/articles/PMC8003360/ /pubmed/33808759 http://dx.doi.org/10.3390/pharmaceutics13030408 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Kim, Eui Jin
Seo, Ji Bin
Yu, Jae Sik
Lee, Seoyoung
Lim, Jae Sung
Choi, Jeong Uk
Lee, Chang-Min
Rashan, Luay
Kim, Ki Hyun
Cho, Young-Chang
Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title_full Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title_fullStr Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title_full_unstemmed Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title_short Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4′-O-Digallate, from Woodfordia uniflora by Regulating NF-κB Signaling Pathway in Mouse Macrophages
title_sort anti-inflammatory effects of a polyphenol, catechin-7,4′-o-digallate, from woodfordia uniflora by regulating nf-κb signaling pathway in mouse macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003360/
https://www.ncbi.nlm.nih.gov/pubmed/33808759
http://dx.doi.org/10.3390/pharmaceutics13030408
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