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Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize
Improving sulfur assimilation in maize kernels is essential due to humans and animals’ inability to synthesize methionine. Serine acetyltransferase (SAT) is a critical enzyme that controls cystine biosynthesis in plants. In this study, all SAT gene members were genome-wide characterized by using a s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003530/ https://www.ncbi.nlm.nih.gov/pubmed/33808582 http://dx.doi.org/10.3390/genes12030437 |
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author | Zhao, Zhixuan Li, Shuai Ji, Chen Zhou, Yong Li, Changsheng Wang, Wenqin |
author_facet | Zhao, Zhixuan Li, Shuai Ji, Chen Zhou, Yong Li, Changsheng Wang, Wenqin |
author_sort | Zhao, Zhixuan |
collection | PubMed |
description | Improving sulfur assimilation in maize kernels is essential due to humans and animals’ inability to synthesize methionine. Serine acetyltransferase (SAT) is a critical enzyme that controls cystine biosynthesis in plants. In this study, all SAT gene members were genome-wide characterized by using a sequence homology search. The RNA-seq quantification indicates that they are highly expressed in leaves, other than root and seeds, consistent with their biological functions in sulfur assimilation. With the recently released 25 genomes of nested association mapping (NAM) founders representing the diverse maize stock, we had the opportunity to investigate the SAT genetic variation comprehensively. The abundant transposon insertions into SAT genes indicate their driving power in terms of gene structure and genome evolution. We found that the transposon insertion into exons could change SAT gene transcription, whereas there was no significant correlation between transposable element (TE) insertion into introns and their gene expression, indicating that other regulatory elements such as promoters could also be involved. Understanding the SAT gene structure, gene expression and genetic variation involved in natural selection and species adaption could precisely guide genetic engineering to manipulate sulfur assimilation in maize and to improve nutritional quality. |
format | Online Article Text |
id | pubmed-8003530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80035302021-03-28 Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize Zhao, Zhixuan Li, Shuai Ji, Chen Zhou, Yong Li, Changsheng Wang, Wenqin Genes (Basel) Article Improving sulfur assimilation in maize kernels is essential due to humans and animals’ inability to synthesize methionine. Serine acetyltransferase (SAT) is a critical enzyme that controls cystine biosynthesis in plants. In this study, all SAT gene members were genome-wide characterized by using a sequence homology search. The RNA-seq quantification indicates that they are highly expressed in leaves, other than root and seeds, consistent with their biological functions in sulfur assimilation. With the recently released 25 genomes of nested association mapping (NAM) founders representing the diverse maize stock, we had the opportunity to investigate the SAT genetic variation comprehensively. The abundant transposon insertions into SAT genes indicate their driving power in terms of gene structure and genome evolution. We found that the transposon insertion into exons could change SAT gene transcription, whereas there was no significant correlation between transposable element (TE) insertion into introns and their gene expression, indicating that other regulatory elements such as promoters could also be involved. Understanding the SAT gene structure, gene expression and genetic variation involved in natural selection and species adaption could precisely guide genetic engineering to manipulate sulfur assimilation in maize and to improve nutritional quality. MDPI 2021-03-19 /pmc/articles/PMC8003530/ /pubmed/33808582 http://dx.doi.org/10.3390/genes12030437 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Zhao, Zhixuan Li, Shuai Ji, Chen Zhou, Yong Li, Changsheng Wang, Wenqin Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title | Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title_full | Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title_fullStr | Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title_full_unstemmed | Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title_short | Genetic Variation of the Serine Acetyltransferase Gene Family for Sulfur Assimilation in Maize |
title_sort | genetic variation of the serine acetyltransferase gene family for sulfur assimilation in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003530/ https://www.ncbi.nlm.nih.gov/pubmed/33808582 http://dx.doi.org/10.3390/genes12030437 |
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