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N-Glycomics of Cerebrospinal Fluid: Method Comparison
Cerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated diff...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003558/ https://www.ncbi.nlm.nih.gov/pubmed/33808573 http://dx.doi.org/10.3390/molecules26061712 |
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author | Cho, Byeong Gwan Gutierrez Reyes, Cristian D. Mechref, Yehia |
author_facet | Cho, Byeong Gwan Gutierrez Reyes, Cristian D. Mechref, Yehia |
author_sort | Cho, Byeong Gwan |
collection | PubMed |
description | Cerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated different N-glycan sample preparation approaches to develop a more sensitive method. These methods, one with an increased amount of buffer solution during the N-glycan release step with a lower amount of sample volume and the other with Filter-Aided N-Glycan Separation (FANGS), were compared with recent work to demonstrate their effectiveness. It was demonstrated that an increased amount of buffer solution showed higher intensity in comparison to the previously published method and FANGS. This suggested that digestion efficiency during the N-glycan release step was not in an optimal condition from the previously published method, and that there is a substantial loss of sample with FANGS when preparing N-glycans from CSF. |
format | Online Article Text |
id | pubmed-8003558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80035582021-03-28 N-Glycomics of Cerebrospinal Fluid: Method Comparison Cho, Byeong Gwan Gutierrez Reyes, Cristian D. Mechref, Yehia Molecules Communication Cerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated different N-glycan sample preparation approaches to develop a more sensitive method. These methods, one with an increased amount of buffer solution during the N-glycan release step with a lower amount of sample volume and the other with Filter-Aided N-Glycan Separation (FANGS), were compared with recent work to demonstrate their effectiveness. It was demonstrated that an increased amount of buffer solution showed higher intensity in comparison to the previously published method and FANGS. This suggested that digestion efficiency during the N-glycan release step was not in an optimal condition from the previously published method, and that there is a substantial loss of sample with FANGS when preparing N-glycans from CSF. MDPI 2021-03-19 /pmc/articles/PMC8003558/ /pubmed/33808573 http://dx.doi.org/10.3390/molecules26061712 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Cho, Byeong Gwan Gutierrez Reyes, Cristian D. Mechref, Yehia N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title | N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title_full | N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title_fullStr | N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title_full_unstemmed | N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title_short | N-Glycomics of Cerebrospinal Fluid: Method Comparison |
title_sort | n-glycomics of cerebrospinal fluid: method comparison |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003558/ https://www.ncbi.nlm.nih.gov/pubmed/33808573 http://dx.doi.org/10.3390/molecules26061712 |
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