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High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children

Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in ch...

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Autores principales: Huber, Michael, Schreiber, Peter Werner, Scheier, Thomas, Audigé, Annette, Buonomano, Roberto, Rudiger, Alain, Braun, Dominique L., Eich, Gerhard, Keller, Dagmar I., Hasse, Barbara, Böni, Jürg, Berger, Christoph, Günthard, Huldrych F., Manrique, Amapola, Trkola, Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003663/
https://www.ncbi.nlm.nih.gov/pubmed/33808815
http://dx.doi.org/10.3390/microorganisms9030642
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author Huber, Michael
Schreiber, Peter Werner
Scheier, Thomas
Audigé, Annette
Buonomano, Roberto
Rudiger, Alain
Braun, Dominique L.
Eich, Gerhard
Keller, Dagmar I.
Hasse, Barbara
Böni, Jürg
Berger, Christoph
Günthard, Huldrych F.
Manrique, Amapola
Trkola, Alexandra
author_facet Huber, Michael
Schreiber, Peter Werner
Scheier, Thomas
Audigé, Annette
Buonomano, Roberto
Rudiger, Alain
Braun, Dominique L.
Eich, Gerhard
Keller, Dagmar I.
Hasse, Barbara
Böni, Jürg
Berger, Christoph
Günthard, Huldrych F.
Manrique, Amapola
Trkola, Alexandra
author_sort Huber, Michael
collection PubMed
description Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in children, is missing. We conducted a large-scale head-to-head comparison of SARS-CoV-2 detection by RT-PCR in saliva and NPS of 1270 adults and children reporting to outpatient test centers and an emergency unit. In total, 273 individuals were tested positive for SARS-CoV-2 in either NPS or saliva. SARS-CoV-2 RT-PCR results in the two specimens showed a high agreement (overall percent agreement = 97.8%). Despite lower viral loads in the saliva of both adults and children, detection of SARS-CoV-2 in saliva fared well compared to NPS (positive percent agreement = 92.5%). Importantly, in children, SARS-CoV-2 infections were more often detected in saliva than NPS (positive predictive value = 84.8%), underlining that NPS sampling in children can be challenging. The comprehensive parallel analysis reported here establishes saliva as a generally reliable specimen for the detection of SARS-CoV-2, with particular advantages for testing children, that is readily applicable to increase and facilitate repetitive and mass testing in adults and children.
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spelling pubmed-80036632021-03-28 High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children Huber, Michael Schreiber, Peter Werner Scheier, Thomas Audigé, Annette Buonomano, Roberto Rudiger, Alain Braun, Dominique L. Eich, Gerhard Keller, Dagmar I. Hasse, Barbara Böni, Jürg Berger, Christoph Günthard, Huldrych F. Manrique, Amapola Trkola, Alexandra Microorganisms Article Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in children, is missing. We conducted a large-scale head-to-head comparison of SARS-CoV-2 detection by RT-PCR in saliva and NPS of 1270 adults and children reporting to outpatient test centers and an emergency unit. In total, 273 individuals were tested positive for SARS-CoV-2 in either NPS or saliva. SARS-CoV-2 RT-PCR results in the two specimens showed a high agreement (overall percent agreement = 97.8%). Despite lower viral loads in the saliva of both adults and children, detection of SARS-CoV-2 in saliva fared well compared to NPS (positive percent agreement = 92.5%). Importantly, in children, SARS-CoV-2 infections were more often detected in saliva than NPS (positive predictive value = 84.8%), underlining that NPS sampling in children can be challenging. The comprehensive parallel analysis reported here establishes saliva as a generally reliable specimen for the detection of SARS-CoV-2, with particular advantages for testing children, that is readily applicable to increase and facilitate repetitive and mass testing in adults and children. MDPI 2021-03-19 /pmc/articles/PMC8003663/ /pubmed/33808815 http://dx.doi.org/10.3390/microorganisms9030642 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Huber, Michael
Schreiber, Peter Werner
Scheier, Thomas
Audigé, Annette
Buonomano, Roberto
Rudiger, Alain
Braun, Dominique L.
Eich, Gerhard
Keller, Dagmar I.
Hasse, Barbara
Böni, Jürg
Berger, Christoph
Günthard, Huldrych F.
Manrique, Amapola
Trkola, Alexandra
High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title_full High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title_fullStr High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title_full_unstemmed High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title_short High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
title_sort high efficacy of saliva in detecting sars-cov-2 by rt-pcr in adults and children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003663/
https://www.ncbi.nlm.nih.gov/pubmed/33808815
http://dx.doi.org/10.3390/microorganisms9030642
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