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High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in ch...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003663/ https://www.ncbi.nlm.nih.gov/pubmed/33808815 http://dx.doi.org/10.3390/microorganisms9030642 |
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author | Huber, Michael Schreiber, Peter Werner Scheier, Thomas Audigé, Annette Buonomano, Roberto Rudiger, Alain Braun, Dominique L. Eich, Gerhard Keller, Dagmar I. Hasse, Barbara Böni, Jürg Berger, Christoph Günthard, Huldrych F. Manrique, Amapola Trkola, Alexandra |
author_facet | Huber, Michael Schreiber, Peter Werner Scheier, Thomas Audigé, Annette Buonomano, Roberto Rudiger, Alain Braun, Dominique L. Eich, Gerhard Keller, Dagmar I. Hasse, Barbara Böni, Jürg Berger, Christoph Günthard, Huldrych F. Manrique, Amapola Trkola, Alexandra |
author_sort | Huber, Michael |
collection | PubMed |
description | Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in children, is missing. We conducted a large-scale head-to-head comparison of SARS-CoV-2 detection by RT-PCR in saliva and NPS of 1270 adults and children reporting to outpatient test centers and an emergency unit. In total, 273 individuals were tested positive for SARS-CoV-2 in either NPS or saliva. SARS-CoV-2 RT-PCR results in the two specimens showed a high agreement (overall percent agreement = 97.8%). Despite lower viral loads in the saliva of both adults and children, detection of SARS-CoV-2 in saliva fared well compared to NPS (positive percent agreement = 92.5%). Importantly, in children, SARS-CoV-2 infections were more often detected in saliva than NPS (positive predictive value = 84.8%), underlining that NPS sampling in children can be challenging. The comprehensive parallel analysis reported here establishes saliva as a generally reliable specimen for the detection of SARS-CoV-2, with particular advantages for testing children, that is readily applicable to increase and facilitate repetitive and mass testing in adults and children. |
format | Online Article Text |
id | pubmed-8003663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80036632021-03-28 High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children Huber, Michael Schreiber, Peter Werner Scheier, Thomas Audigé, Annette Buonomano, Roberto Rudiger, Alain Braun, Dominique L. Eich, Gerhard Keller, Dagmar I. Hasse, Barbara Böni, Jürg Berger, Christoph Günthard, Huldrych F. Manrique, Amapola Trkola, Alexandra Microorganisms Article Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in children, is missing. We conducted a large-scale head-to-head comparison of SARS-CoV-2 detection by RT-PCR in saliva and NPS of 1270 adults and children reporting to outpatient test centers and an emergency unit. In total, 273 individuals were tested positive for SARS-CoV-2 in either NPS or saliva. SARS-CoV-2 RT-PCR results in the two specimens showed a high agreement (overall percent agreement = 97.8%). Despite lower viral loads in the saliva of both adults and children, detection of SARS-CoV-2 in saliva fared well compared to NPS (positive percent agreement = 92.5%). Importantly, in children, SARS-CoV-2 infections were more often detected in saliva than NPS (positive predictive value = 84.8%), underlining that NPS sampling in children can be challenging. The comprehensive parallel analysis reported here establishes saliva as a generally reliable specimen for the detection of SARS-CoV-2, with particular advantages for testing children, that is readily applicable to increase and facilitate repetitive and mass testing in adults and children. MDPI 2021-03-19 /pmc/articles/PMC8003663/ /pubmed/33808815 http://dx.doi.org/10.3390/microorganisms9030642 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Huber, Michael Schreiber, Peter Werner Scheier, Thomas Audigé, Annette Buonomano, Roberto Rudiger, Alain Braun, Dominique L. Eich, Gerhard Keller, Dagmar I. Hasse, Barbara Böni, Jürg Berger, Christoph Günthard, Huldrych F. Manrique, Amapola Trkola, Alexandra High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title | High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title_full | High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title_fullStr | High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title_full_unstemmed | High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title_short | High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children |
title_sort | high efficacy of saliva in detecting sars-cov-2 by rt-pcr in adults and children |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003663/ https://www.ncbi.nlm.nih.gov/pubmed/33808815 http://dx.doi.org/10.3390/microorganisms9030642 |
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