Cargando…

Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury

Antioxidant agents are promising pharmaceuticals to prevent salivary gland (SG) epithelial injury from radiotherapy and their associated irreversible dry mouth symptoms. Epigallocatechin-3-gallate (EGCG) is a well-known antioxidant that can exert growth or inhibitory biological effects in normal or...

Descripción completa

Detalles Bibliográficos
Autores principales: Sulistiyani, Erni, Brimson, James M., Chansaenroj, Ajjima, Sariya, Ladawan, Urkasemsin, Ganokon, Oonsiri, Sornjarod, Tencomnao, Tewin, Vacharaksa, Anjalee, Chaisuparat, Risa, Ferreira, Joao N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003734/
https://www.ncbi.nlm.nih.gov/pubmed/33808935
http://dx.doi.org/10.3390/ijms22063162
_version_ 1783671759293120512
author Sulistiyani, Erni
Brimson, James M.
Chansaenroj, Ajjima
Sariya, Ladawan
Urkasemsin, Ganokon
Oonsiri, Sornjarod
Tencomnao, Tewin
Vacharaksa, Anjalee
Chaisuparat, Risa
Ferreira, Joao N.
author_facet Sulistiyani, Erni
Brimson, James M.
Chansaenroj, Ajjima
Sariya, Ladawan
Urkasemsin, Ganokon
Oonsiri, Sornjarod
Tencomnao, Tewin
Vacharaksa, Anjalee
Chaisuparat, Risa
Ferreira, Joao N.
author_sort Sulistiyani, Erni
collection PubMed
description Antioxidant agents are promising pharmaceuticals to prevent salivary gland (SG) epithelial injury from radiotherapy and their associated irreversible dry mouth symptoms. Epigallocatechin-3-gallate (EGCG) is a well-known antioxidant that can exert growth or inhibitory biological effects in normal or pathological tissues leading to disease prevention. The effects of EGCG in the various SG epithelial compartments are poorly understood during homeostasis and upon radiation (IR) injury. This study aims to: (1) determine whether EGCG can support epithelial proliferation during homeostasis; and (2) investigate what epithelial cells are protected by EGCG from IR injury. Ex vivo mouse SG were treated with EGCG from 7.5–30 µg/mL for up to 72 h. Next, SG epithelial branching morphogenesis was evaluated by bright-field microscopy, immunofluorescence, and gene expression arrays. To establish IR injury models, linear accelerator (LINAC) technologies were utilized, and radiation doses optimized. EGCG epithelial effects in these injury models were assessed using light, confocal and electron microscopy, the Griess assay, immunohistochemistry, and gene arrays. SG pretreated with EGCG 7.5 µg/mL promoted epithelial proliferation and the development of pro-acinar buds and ducts in regular homeostasis. Furthermore, EGCG increased the populations of epithelial progenitors in buds and ducts and pro-acinar cells, most probably due to its observed antioxidant activity after IR injury, which prevented epithelial apoptosis. Future studies will assess the potential for nanocarriers to increase the oral bioavailability of EGCG.
format Online
Article
Text
id pubmed-8003734
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80037342021-03-28 Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury Sulistiyani, Erni Brimson, James M. Chansaenroj, Ajjima Sariya, Ladawan Urkasemsin, Ganokon Oonsiri, Sornjarod Tencomnao, Tewin Vacharaksa, Anjalee Chaisuparat, Risa Ferreira, Joao N. Int J Mol Sci Article Antioxidant agents are promising pharmaceuticals to prevent salivary gland (SG) epithelial injury from radiotherapy and their associated irreversible dry mouth symptoms. Epigallocatechin-3-gallate (EGCG) is a well-known antioxidant that can exert growth or inhibitory biological effects in normal or pathological tissues leading to disease prevention. The effects of EGCG in the various SG epithelial compartments are poorly understood during homeostasis and upon radiation (IR) injury. This study aims to: (1) determine whether EGCG can support epithelial proliferation during homeostasis; and (2) investigate what epithelial cells are protected by EGCG from IR injury. Ex vivo mouse SG were treated with EGCG from 7.5–30 µg/mL for up to 72 h. Next, SG epithelial branching morphogenesis was evaluated by bright-field microscopy, immunofluorescence, and gene expression arrays. To establish IR injury models, linear accelerator (LINAC) technologies were utilized, and radiation doses optimized. EGCG epithelial effects in these injury models were assessed using light, confocal and electron microscopy, the Griess assay, immunohistochemistry, and gene arrays. SG pretreated with EGCG 7.5 µg/mL promoted epithelial proliferation and the development of pro-acinar buds and ducts in regular homeostasis. Furthermore, EGCG increased the populations of epithelial progenitors in buds and ducts and pro-acinar cells, most probably due to its observed antioxidant activity after IR injury, which prevented epithelial apoptosis. Future studies will assess the potential for nanocarriers to increase the oral bioavailability of EGCG. MDPI 2021-03-19 /pmc/articles/PMC8003734/ /pubmed/33808935 http://dx.doi.org/10.3390/ijms22063162 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sulistiyani, Erni
Brimson, James M.
Chansaenroj, Ajjima
Sariya, Ladawan
Urkasemsin, Ganokon
Oonsiri, Sornjarod
Tencomnao, Tewin
Vacharaksa, Anjalee
Chaisuparat, Risa
Ferreira, Joao N.
Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title_full Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title_fullStr Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title_full_unstemmed Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title_short Epigallocatechin-3-Gallate Protects Pro-Acinar Epithelia Against Salivary Gland Radiation Injury
title_sort epigallocatechin-3-gallate protects pro-acinar epithelia against salivary gland radiation injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003734/
https://www.ncbi.nlm.nih.gov/pubmed/33808935
http://dx.doi.org/10.3390/ijms22063162
work_keys_str_mv AT sulistiyanierni epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT brimsonjamesm epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT chansaenrojajjima epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT sariyaladawan epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT urkasemsinganokon epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT oonsirisornjarod epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT tencomnaotewin epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT vacharaksaanjalee epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT chaisuparatrisa epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury
AT ferreirajoaon epigallocatechin3gallateprotectsproacinarepitheliaagainstsalivaryglandradiationinjury