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Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy

In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X...

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Autores principales: Caro, Carlos, Gámez, Francisco, Quaresma, Pedro, Páez-Muñoz, Jose María, Domínguez, Alejandro, Pearson, John R., Pernía Leal, Manuel, Beltrán, Ana M., Fernandez-Afonso, Yilian, De la Fuente, Jesús M., Franco, Ricardo, Pereira, Eulália, García-Martín, Maria Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003746/
https://www.ncbi.nlm.nih.gov/pubmed/33804636
http://dx.doi.org/10.3390/pharmaceutics13030416
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author Caro, Carlos
Gámez, Francisco
Quaresma, Pedro
Páez-Muñoz, Jose María
Domínguez, Alejandro
Pearson, John R.
Pernía Leal, Manuel
Beltrán, Ana M.
Fernandez-Afonso, Yilian
De la Fuente, Jesús M.
Franco, Ricardo
Pereira, Eulália
García-Martín, Maria Luisa
author_facet Caro, Carlos
Gámez, Francisco
Quaresma, Pedro
Páez-Muñoz, Jose María
Domínguez, Alejandro
Pearson, John R.
Pernía Leal, Manuel
Beltrán, Ana M.
Fernandez-Afonso, Yilian
De la Fuente, Jesús M.
Franco, Ricardo
Pereira, Eulália
García-Martín, Maria Luisa
author_sort Caro, Carlos
collection PubMed
description In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model.
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spelling pubmed-80037462021-03-28 Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy Caro, Carlos Gámez, Francisco Quaresma, Pedro Páez-Muñoz, Jose María Domínguez, Alejandro Pearson, John R. Pernía Leal, Manuel Beltrán, Ana M. Fernandez-Afonso, Yilian De la Fuente, Jesús M. Franco, Ricardo Pereira, Eulália García-Martín, Maria Luisa Pharmaceutics Article In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model. MDPI 2021-03-20 /pmc/articles/PMC8003746/ /pubmed/33804636 http://dx.doi.org/10.3390/pharmaceutics13030416 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Caro, Carlos
Gámez, Francisco
Quaresma, Pedro
Páez-Muñoz, Jose María
Domínguez, Alejandro
Pearson, John R.
Pernía Leal, Manuel
Beltrán, Ana M.
Fernandez-Afonso, Yilian
De la Fuente, Jesús M.
Franco, Ricardo
Pereira, Eulália
García-Martín, Maria Luisa
Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title_full Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title_fullStr Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title_full_unstemmed Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title_short Fe(3)O(4)-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
title_sort fe(3)o(4)-au core-shell nanoparticles as a multimodal platform for in vivo imaging and focused photothermal therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003746/
https://www.ncbi.nlm.nih.gov/pubmed/33804636
http://dx.doi.org/10.3390/pharmaceutics13030416
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