Augmenting Nesidiocoris tenuis (Nesidiocoris) with a Factitious Diet of Artemia Cysts to Control Bemisia tabaci (Gennadius) on Tomato Plants under Greenhouse Conditions

SIMPLE SUMMARY: Nesidiocoris tenuis (Hemiptera: Miridae) is an important biocontrol agent of several key arthropod pests, such as greenhouse and tobacco whiteflies, the South American tomato pinworm, thrips, plant mites, and other pests in the greenhouse. However, optimizing N. tenuis utilization in the greenhouse to control greenhouse pests such as whiteflies still needs further studies, especially in Japan. Here, we showed that factitious supplementary dietary in the form of Artemia cysts enhanced with high fructose corn syrup and honey, and delivered using a hemp rope could promote N. tenuis proliferation and spread among tomato plants. Nesidiocoris tenuis spread among tomato plants therefore, can maintain whitefly (Bemisia tabaci) eggs and nymph numbers at minimum in the greenhouse conditions. ABSTRACT: Natural predators such as Nesidiocoris tenuis are known for their role in managing greenhouse pests. However, techniques in maximizing the biological control potential of N. tenuis under field conditions are still lacking. We evaluated under greenhouse conditions the prospects of Artemia cysts enhanced with high fructose corn syrup and honey, and delivered using hemp strings (hemp rope) as supplementary factitious dietary in augmenting the proliferation and spread of N. tenuis on tomato plants. Results showed that N. tenuis supplemented with hemp rope could establish, proliferate and disperse among tomato plants compared to the N. tenuis supplemented with banker plants. Even though N. tenuis proliferated exponentially on banker plants, their movement and relocation to tomato plants, as expected, were only congested on tomato plants near the banker plants. However, as the survey continued, they relocated to the rest of the tomato plants. Furthermore, the number of Bemisia tabaci eggs and nymphs, a serious greenhouse pest of tomato, was observed to be significantly reduced in hemp rope greenhouse compared to banker plants and the negative control (no pest control system) greenhouses. This study, therefore, establishes foundational data on the usage of Artemia cysts enhanced with isomerized sugar (high fructose corn syrup) and honey under greenhouse conditions as factitious supplementary dietary in supporting N. tenuis establishment and spread, traits that are essential towards development of whitefly Integrated Pest Management (IPM) system. enhanced with isomerized sugar (high fructose corn syrup) and honey.