Cargando…
Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia
Japanese Encephalitis (JE) is the most important cause of human encephalitis in Southeast Asia, and this zoonosis is mainly transmitted from pigs to human by mosquitoes. A better understanding of the host-feeding preference of Japanese encephalitis virus (JEV) major vectors is crucial for identifyin...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003966/ https://www.ncbi.nlm.nih.gov/pubmed/33800999 http://dx.doi.org/10.3390/pathogens10030376 |
_version_ | 1783671813886181376 |
---|---|
author | Boyer, Sébastien Durand, Benoit Yean, Sony Brengues, Cécile Maquart, Pierre-Olivier Fontenille, Didier Chevalier, Véronique |
author_facet | Boyer, Sébastien Durand, Benoit Yean, Sony Brengues, Cécile Maquart, Pierre-Olivier Fontenille, Didier Chevalier, Véronique |
author_sort | Boyer, Sébastien |
collection | PubMed |
description | Japanese Encephalitis (JE) is the most important cause of human encephalitis in Southeast Asia, and this zoonosis is mainly transmitted from pigs to human by mosquitoes. A better understanding of the host-feeding preference of Japanese encephalitis virus (JEV) major vectors is crucial for identifying risk areas, defining bridge vector species and targeting adapted vector control strategies. To assess host-feeding preference of JE vectors in a rural Cambodian area where JE is known to circulate, in 2017, we implemented four sessions of mosquito trapping (March, June, September, December), during five consecutive nights, collecting four times a night (6 p.m. to 6 a.m.), and using five baited traps simultaneously, i.e., cow, chicken, pig, human, and a blank one for control. In addition, blood meals of 157 engorged females trapped at the same location were opportunistically analyzed with polymerase chain reaction (PCR), using cow, pig, human, and dog blood primers. More than 95% of the 36,709 trapped mosquitoes were potential JE vectors. These vectors were trapped in large numbers throughout the year, including during the dry season, and from 6 p.m. to 6 a.m. Despite the apparent host-feeding preference of Culex vishnui, Cx. gelidus, and Cx. tritaenhyorhincus for cows, statistical analysis suggested that the primary target of these three mosquito species were pigs. Dog blood was detected in eight mosquitoes of the 157 tested, showing that mosquitoes also bite dogs, and suggesting that dogs may be used as proxy of the risk for human to get infected by JE virus. |
format | Online Article Text |
id | pubmed-8003966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80039662021-03-28 Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia Boyer, Sébastien Durand, Benoit Yean, Sony Brengues, Cécile Maquart, Pierre-Olivier Fontenille, Didier Chevalier, Véronique Pathogens Article Japanese Encephalitis (JE) is the most important cause of human encephalitis in Southeast Asia, and this zoonosis is mainly transmitted from pigs to human by mosquitoes. A better understanding of the host-feeding preference of Japanese encephalitis virus (JEV) major vectors is crucial for identifying risk areas, defining bridge vector species and targeting adapted vector control strategies. To assess host-feeding preference of JE vectors in a rural Cambodian area where JE is known to circulate, in 2017, we implemented four sessions of mosquito trapping (March, June, September, December), during five consecutive nights, collecting four times a night (6 p.m. to 6 a.m.), and using five baited traps simultaneously, i.e., cow, chicken, pig, human, and a blank one for control. In addition, blood meals of 157 engorged females trapped at the same location were opportunistically analyzed with polymerase chain reaction (PCR), using cow, pig, human, and dog blood primers. More than 95% of the 36,709 trapped mosquitoes were potential JE vectors. These vectors were trapped in large numbers throughout the year, including during the dry season, and from 6 p.m. to 6 a.m. Despite the apparent host-feeding preference of Culex vishnui, Cx. gelidus, and Cx. tritaenhyorhincus for cows, statistical analysis suggested that the primary target of these three mosquito species were pigs. Dog blood was detected in eight mosquitoes of the 157 tested, showing that mosquitoes also bite dogs, and suggesting that dogs may be used as proxy of the risk for human to get infected by JE virus. MDPI 2021-03-21 /pmc/articles/PMC8003966/ /pubmed/33800999 http://dx.doi.org/10.3390/pathogens10030376 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Boyer, Sébastien Durand, Benoit Yean, Sony Brengues, Cécile Maquart, Pierre-Olivier Fontenille, Didier Chevalier, Véronique Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title | Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title_full | Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title_fullStr | Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title_full_unstemmed | Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title_short | Host-Feeding Preference and Diel Activity of Mosquito Vectors of the Japanese Encephalitis Virus in Rural Cambodia |
title_sort | host-feeding preference and diel activity of mosquito vectors of the japanese encephalitis virus in rural cambodia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003966/ https://www.ncbi.nlm.nih.gov/pubmed/33800999 http://dx.doi.org/10.3390/pathogens10030376 |
work_keys_str_mv | AT boyersebastien hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT durandbenoit hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT yeansony hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT brenguescecile hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT maquartpierreolivier hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT fontenilledidier hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia AT chevalierveronique hostfeedingpreferenceanddielactivityofmosquitovectorsofthejapaneseencephalitisvirusinruralcambodia |