Effects of L-Glutamine Supplementation during the Gestation of Gilts and Sows on the Offspring Development in a Traditional Swine Breed

SIMPLE SUMMARY: Nutritional strategies during pregnancy in swine production are considered essential to increase the number of piglets born alive and improve their survival and development. Amino acids, such as glutamine, are among the best compound to introduce in commercial farms after obtaining positive results in trials carried out in selected swine breeds. However, several critical productive factors have to be assessed before translating these strategies to the farm level to ensure the best balance between benefits and investments. The current study focused on the effects of prenatal L-glutamine supplementation on the offspring of Iberian gilts and sows under farm conditions. It is the first trial of amino acid supplementation during pregnancy carried out in traditional swine breeds. These non-selected swine breeds show productive or physiological differences that could affect the supplementation effect. Indeed, although there were changes at the molecular and tissue level, these effects did not turn into advantageous effects for the offspring of traditional breeds. The present study shows the importance of pre-testing nutritional strategies under the final conditions and breeds of implementation and the need to deepen at the molecular level to improve the biological interpretation of findings. ABSTRACT: The use of amino acids during pregnancy, such as glutamine (Gln), seems to be a promising strategy in selected swine breeds to improve the offspring prenatal development. The main goal of the current study was to assess the development of the offspring from parity 1–3 sows of a traditional breed, which were supplemented with 1% glutamine after Day 35 of gestation, under farm conditions. A total of 486 (288 treated) piglets from 78 (46 treated) Iberian sows were used. At birth and slaughterhouse, fatty acid composition, metabolism, and mTOR pathway gene expression were analyzed. At birth, treated newborns showed greater amounts of specific amino acids in plasma, such as glutamine, asparagine, or alanine, and Σn-3 fatty acids in cellular membranes than control newborns. The expression of genes belonging to mTOR Complex 1 was also higher in treated piglets with normal birth-weight. However, these findings did not improve productive traits at birth or following periods in litters from supplemented gilts (parity 1) or sows (parities 2–3). Thus, further research is needed to properly understand the effects of prenatal glutamine supplementation, particularly in traditional swine breeds.