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Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity

Green synthesis of gold nanoparticles (GNPs) with plant extracts has gained considerable interest in the field of biomedicine. Recently, the bioreduction nature of herbal extracts has helped to synthesize spherical GNPs of different potential from gold salt. In this study, a fast ecofriendly method...

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Autores principales: Al Saqr, Ahmed, Khafagy, El-Sayed, Alalaiwe, Ahmed, Aldawsari, Mohammed F., Alshahrani, Saad M., Anwer, Md. Khalid, Khan, Salman, Lila, Amr S. Abu, Arab, Hany H., Hegazy, Wael A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004202/
https://www.ncbi.nlm.nih.gov/pubmed/33809859
http://dx.doi.org/10.3390/nano11030808
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author Al Saqr, Ahmed
Khafagy, El-Sayed
Alalaiwe, Ahmed
Aldawsari, Mohammed F.
Alshahrani, Saad M.
Anwer, Md. Khalid
Khan, Salman
Lila, Amr S. Abu
Arab, Hany H.
Hegazy, Wael A. H.
author_facet Al Saqr, Ahmed
Khafagy, El-Sayed
Alalaiwe, Ahmed
Aldawsari, Mohammed F.
Alshahrani, Saad M.
Anwer, Md. Khalid
Khan, Salman
Lila, Amr S. Abu
Arab, Hany H.
Hegazy, Wael A. H.
author_sort Al Saqr, Ahmed
collection PubMed
description Green synthesis of gold nanoparticles (GNPs) with plant extracts has gained considerable interest in the field of biomedicine. Recently, the bioreduction nature of herbal extracts has helped to synthesize spherical GNPs of different potential from gold salt. In this study, a fast ecofriendly method was adopted for the synthesis of GNPs using fresh peel (aqueous) extracts of Benincasa hispida, which acted as reducing and stabilizing agents. The biosynthesized GNPs were characterized by UV–VIS and Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering. In addition, the in vitro antibacterial and anticancer activities of synthesized GNPs were investigated. The formation of gold nanoparticles was confirmed by the existence of a sharp absorption peak at 520 nm, corresponding to the surface plasmon resonance (SPR) band of the GNPs. TEM analysis revealed that the prepared GNPs were spherical in shape and had an average particle size of 22.18 ± 2 nm. Most importantly, the synthesized GNPs exhibited considerable antibacterial activity against different Gram-positive and Gram-negative bacteria. Furthermore, the biosynthesized GNPs exerted remarkable in vitro cytotoxicity against human cervical cancer cell line, while sparing normal human primary osteoblast cells. Such cytotoxic effect was attributed to the increased production of reactive oxygen species (ROS) that contributed to the damage of HeLa cells. Collectively, peel extracts of B. hispida can be efficiently used for the synthesis of GNPs, which can be adopted as a natural source of antimicrobial and anticancer agent.
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spelling pubmed-80042022021-03-28 Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity Al Saqr, Ahmed Khafagy, El-Sayed Alalaiwe, Ahmed Aldawsari, Mohammed F. Alshahrani, Saad M. Anwer, Md. Khalid Khan, Salman Lila, Amr S. Abu Arab, Hany H. Hegazy, Wael A. H. Nanomaterials (Basel) Article Green synthesis of gold nanoparticles (GNPs) with plant extracts has gained considerable interest in the field of biomedicine. Recently, the bioreduction nature of herbal extracts has helped to synthesize spherical GNPs of different potential from gold salt. In this study, a fast ecofriendly method was adopted for the synthesis of GNPs using fresh peel (aqueous) extracts of Benincasa hispida, which acted as reducing and stabilizing agents. The biosynthesized GNPs were characterized by UV–VIS and Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering. In addition, the in vitro antibacterial and anticancer activities of synthesized GNPs were investigated. The formation of gold nanoparticles was confirmed by the existence of a sharp absorption peak at 520 nm, corresponding to the surface plasmon resonance (SPR) band of the GNPs. TEM analysis revealed that the prepared GNPs were spherical in shape and had an average particle size of 22.18 ± 2 nm. Most importantly, the synthesized GNPs exhibited considerable antibacterial activity against different Gram-positive and Gram-negative bacteria. Furthermore, the biosynthesized GNPs exerted remarkable in vitro cytotoxicity against human cervical cancer cell line, while sparing normal human primary osteoblast cells. Such cytotoxic effect was attributed to the increased production of reactive oxygen species (ROS) that contributed to the damage of HeLa cells. Collectively, peel extracts of B. hispida can be efficiently used for the synthesis of GNPs, which can be adopted as a natural source of antimicrobial and anticancer agent. MDPI 2021-03-22 /pmc/articles/PMC8004202/ /pubmed/33809859 http://dx.doi.org/10.3390/nano11030808 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Al Saqr, Ahmed
Khafagy, El-Sayed
Alalaiwe, Ahmed
Aldawsari, Mohammed F.
Alshahrani, Saad M.
Anwer, Md. Khalid
Khan, Salman
Lila, Amr S. Abu
Arab, Hany H.
Hegazy, Wael A. H.
Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title_full Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title_fullStr Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title_full_unstemmed Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title_short Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity
title_sort synthesis of gold nanoparticles by using green machinery: characterization and in vitro toxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004202/
https://www.ncbi.nlm.nih.gov/pubmed/33809859
http://dx.doi.org/10.3390/nano11030808
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