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Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regu...

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Autores principales: Zang, Xupeng, Zhou, Chen, Wang, Wenjing, Gan, Jianyu, Li, Yaokun, Liu, Dewu, Liu, Guangbin, Hong, Linjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004627/
https://www.ncbi.nlm.nih.gov/pubmed/33810054
http://dx.doi.org/10.3390/biom11030472
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author Zang, Xupeng
Zhou, Chen
Wang, Wenjing
Gan, Jianyu
Li, Yaokun
Liu, Dewu
Liu, Guangbin
Hong, Linjun
author_facet Zang, Xupeng
Zhou, Chen
Wang, Wenjing
Gan, Jianyu
Li, Yaokun
Liu, Dewu
Liu, Guangbin
Hong, Linjun
author_sort Zang, Xupeng
collection PubMed
description Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regulatory mechanisms associated with establishing endometrial receptivity through integrating bioinformatics analysis and experimental verification. MiRNA expression profiles were obtained by high-throughput sequencing, resulting in the detection of 33 differentially expressed miRNAs (DEMs), followed by their validation through quantitative RT-PCR. Furthermore, 10 potential transcription factors (TFs) and 1316 target genes of these DEMs were obtained, and the TF–miRNA and miRNA–mRNA interaction networks were constructed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these miRNAs were significantly linked to establishing endometrial receptivity. Moreover, the fluorescence in situ hybridization (FISH) analysis, dual-luciferase report assay, and immunohistochemistry (IHC) analysis corroborated that chi-miR-483 could directly bind to deltex E3 ubiquitin ligase 3L (DTX3L) to reduce its expression level. In conclusion, our findings contribute to a better understanding of molecular mechanisms regulating the endometrial receptivity of goats, and they provide a reference for improving embryo implantation efficiency.
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spelling pubmed-80046272021-03-29 Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats Zang, Xupeng Zhou, Chen Wang, Wenjing Gan, Jianyu Li, Yaokun Liu, Dewu Liu, Guangbin Hong, Linjun Biomolecules Article Endometrial receptivity represents one of the leading factors affecting the successful implantation of embryos during early pregnancy. However, the mechanism of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. This study was intended to identify potential miRNAs and regulatory mechanisms associated with establishing endometrial receptivity through integrating bioinformatics analysis and experimental verification. MiRNA expression profiles were obtained by high-throughput sequencing, resulting in the detection of 33 differentially expressed miRNAs (DEMs), followed by their validation through quantitative RT-PCR. Furthermore, 10 potential transcription factors (TFs) and 1316 target genes of these DEMs were obtained, and the TF–miRNA and miRNA–mRNA interaction networks were constructed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these miRNAs were significantly linked to establishing endometrial receptivity. Moreover, the fluorescence in situ hybridization (FISH) analysis, dual-luciferase report assay, and immunohistochemistry (IHC) analysis corroborated that chi-miR-483 could directly bind to deltex E3 ubiquitin ligase 3L (DTX3L) to reduce its expression level. In conclusion, our findings contribute to a better understanding of molecular mechanisms regulating the endometrial receptivity of goats, and they provide a reference for improving embryo implantation efficiency. MDPI 2021-03-22 /pmc/articles/PMC8004627/ /pubmed/33810054 http://dx.doi.org/10.3390/biom11030472 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Zang, Xupeng
Zhou, Chen
Wang, Wenjing
Gan, Jianyu
Li, Yaokun
Liu, Dewu
Liu, Guangbin
Hong, Linjun
Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title_full Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title_fullStr Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title_full_unstemmed Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title_short Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats
title_sort differential microrna expression involved in endometrial receptivity of goats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004627/
https://www.ncbi.nlm.nih.gov/pubmed/33810054
http://dx.doi.org/10.3390/biom11030472
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