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In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The...

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Autores principales: Hernández-Gómez, Jimmy G., López-Bonilla, Argelia, Trejo-Tapia, Gabriela, Ávila-Reyes, Sandra V., Jiménez-Aparicio, Antonio R., Hernández-Sánchez, Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004636/
https://www.ncbi.nlm.nih.gov/pubmed/33810002
http://dx.doi.org/10.3390/foods10030674
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author Hernández-Gómez, Jimmy G.
López-Bonilla, Argelia
Trejo-Tapia, Gabriela
Ávila-Reyes, Sandra V.
Jiménez-Aparicio, Antonio R.
Hernández-Sánchez, Humberto
author_facet Hernández-Gómez, Jimmy G.
López-Bonilla, Argelia
Trejo-Tapia, Gabriela
Ávila-Reyes, Sandra V.
Jiménez-Aparicio, Antonio R.
Hernández-Sánchez, Humberto
author_sort Hernández-Gómez, Jimmy G.
collection PubMed
description Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.
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spelling pubmed-80046362021-03-29 In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms Hernández-Gómez, Jimmy G. López-Bonilla, Argelia Trejo-Tapia, Gabriela Ávila-Reyes, Sandra V. Jiménez-Aparicio, Antonio R. Hernández-Sánchez, Humberto Foods Article Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast. MDPI 2021-03-22 /pmc/articles/PMC8004636/ /pubmed/33810002 http://dx.doi.org/10.3390/foods10030674 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Hernández-Gómez, Jimmy G.
López-Bonilla, Argelia
Trejo-Tapia, Gabriela
Ávila-Reyes, Sandra V.
Jiménez-Aparicio, Antonio R.
Hernández-Sánchez, Humberto
In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title_full In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title_fullStr In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title_full_unstemmed In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title_short In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms
title_sort in vitro bile salt hydrolase (bsh) activity screening of different probiotic microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004636/
https://www.ncbi.nlm.nih.gov/pubmed/33810002
http://dx.doi.org/10.3390/foods10030674
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