Cargando…

Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis

Exosomes have attracted considerable attention as drug delivery vehicles because their biological properties can be utilized for selective delivery of therapeutic cargoes to disease sites. In this context, analysis of the in vivo behaviors of exosomes in a diseased state is required to maximize thei...

Descripción completa

Detalles Bibliográficos
Autores principales: Mirzaaghasi, Amin, Han, Yunho, Ahn, So-Hee, Choi, Chulhee, Park, Ji-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004782/
https://www.ncbi.nlm.nih.gov/pubmed/33809966
http://dx.doi.org/10.3390/pharmaceutics13030427
_version_ 1783671981822967808
author Mirzaaghasi, Amin
Han, Yunho
Ahn, So-Hee
Choi, Chulhee
Park, Ji-Ho
author_facet Mirzaaghasi, Amin
Han, Yunho
Ahn, So-Hee
Choi, Chulhee
Park, Ji-Ho
author_sort Mirzaaghasi, Amin
collection PubMed
description Exosomes have attracted considerable attention as drug delivery vehicles because their biological properties can be utilized for selective delivery of therapeutic cargoes to disease sites. In this context, analysis of the in vivo behaviors of exosomes in a diseased state is required to maximize their therapeutic potential as drug delivery vehicles. In this study, we investigated biodistribution and pharmacokinetics of HEK293T cell-derived exosomes and PEGylated liposomes, their synthetic counterparts, into healthy and sepsis mice. We found that biodistribution and pharmacokinetics of exosomes were significantly affected by pathophysiological conditions of sepsis compared to those of liposomes. In the sepsis mice, a substantial number of exosomes were found in the lung after intravenous injection, and their prolonged blood residence was observed due to the liver dysfunction. However, liposomes did not show such sepsis-specific effects significantly. These results demonstrate that exosome-based therapeutics can be developed to manage sepsis and septic shock by virtue of their sepsis-specific in vivo behaviors.
format Online
Article
Text
id pubmed-8004782
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80047822021-03-29 Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis Mirzaaghasi, Amin Han, Yunho Ahn, So-Hee Choi, Chulhee Park, Ji-Ho Pharmaceutics Article Exosomes have attracted considerable attention as drug delivery vehicles because their biological properties can be utilized for selective delivery of therapeutic cargoes to disease sites. In this context, analysis of the in vivo behaviors of exosomes in a diseased state is required to maximize their therapeutic potential as drug delivery vehicles. In this study, we investigated biodistribution and pharmacokinetics of HEK293T cell-derived exosomes and PEGylated liposomes, their synthetic counterparts, into healthy and sepsis mice. We found that biodistribution and pharmacokinetics of exosomes were significantly affected by pathophysiological conditions of sepsis compared to those of liposomes. In the sepsis mice, a substantial number of exosomes were found in the lung after intravenous injection, and their prolonged blood residence was observed due to the liver dysfunction. However, liposomes did not show such sepsis-specific effects significantly. These results demonstrate that exosome-based therapeutics can be developed to manage sepsis and septic shock by virtue of their sepsis-specific in vivo behaviors. MDPI 2021-03-22 /pmc/articles/PMC8004782/ /pubmed/33809966 http://dx.doi.org/10.3390/pharmaceutics13030427 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Mirzaaghasi, Amin
Han, Yunho
Ahn, So-Hee
Choi, Chulhee
Park, Ji-Ho
Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title_full Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title_fullStr Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title_full_unstemmed Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title_short Biodistribution and Pharmacokinectics of Liposomes and Exosomes in a Mouse Model of Sepsis
title_sort biodistribution and pharmacokinectics of liposomes and exosomes in a mouse model of sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004782/
https://www.ncbi.nlm.nih.gov/pubmed/33809966
http://dx.doi.org/10.3390/pharmaceutics13030427
work_keys_str_mv AT mirzaaghasiamin biodistributionandpharmacokinecticsofliposomesandexosomesinamousemodelofsepsis
AT hanyunho biodistributionandpharmacokinecticsofliposomesandexosomesinamousemodelofsepsis
AT ahnsohee biodistributionandpharmacokinecticsofliposomesandexosomesinamousemodelofsepsis
AT choichulhee biodistributionandpharmacokinecticsofliposomesandexosomesinamousemodelofsepsis
AT parkjiho biodistributionandpharmacokinecticsofliposomesandexosomesinamousemodelofsepsis