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Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin

Candida auris is an emerging, multidrug-resistant fungal pathogen that has become a public health threat with an increasing incidence of infections worldwide. Candida auris spreads easily among patients within and between hospitals. Infections and outbreaks caused by C. auris have been reported in t...

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Autores principales: Salah, Husam, Sundararaju, Sathyavathi, Dalil, Lamya, Salameh, Sarah, Al-Wali, Walid, Tang, Patrick, Ben Abid, Fatma, Tsui, Clement K. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004815/
https://www.ncbi.nlm.nih.gov/pubmed/33807036
http://dx.doi.org/10.3390/jof7030240
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author Salah, Husam
Sundararaju, Sathyavathi
Dalil, Lamya
Salameh, Sarah
Al-Wali, Walid
Tang, Patrick
Ben Abid, Fatma
Tsui, Clement K. M.
author_facet Salah, Husam
Sundararaju, Sathyavathi
Dalil, Lamya
Salameh, Sarah
Al-Wali, Walid
Tang, Patrick
Ben Abid, Fatma
Tsui, Clement K. M.
author_sort Salah, Husam
collection PubMed
description Candida auris is an emerging, multidrug-resistant fungal pathogen that has become a public health threat with an increasing incidence of infections worldwide. Candida auris spreads easily among patients within and between hospitals. Infections and outbreaks caused by C. auris have been reported in the Middle East region including Oman, Kuwait, Saudi Arabia, and Qatar; however, the origin of these isolates is largely unknown. Pathogen whole genome sequencing (WGS) was used to determine the epidemiology and drug resistance mutations of C. auris in Qatar. Forty-four samples isolated from patients in three hospitals and the hospital environment were sequenced by Illumina NextSeq. Core genome single nucleotide polymorphisms (SNPs) revealed that all isolates belonged to the South Asian lineage with genetic heterogeneity that suggests previous acquisition from foreign healthcare. The genetic variability among the outbreak isolates in the two hospitals (A and B) was low. Four environmental isolates clustered with the related clinical isolates, and epidemiologically linked isolates clustered together, suggesting that the ongoing transmission of C. auris could be linked to infected/colonized patients and the hospital environment. Prominent mutations Y132F and K143R in ERG11 linked to increased fluconazole resistance were detected.
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spelling pubmed-80048152021-03-29 Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin Salah, Husam Sundararaju, Sathyavathi Dalil, Lamya Salameh, Sarah Al-Wali, Walid Tang, Patrick Ben Abid, Fatma Tsui, Clement K. M. J Fungi (Basel) Article Candida auris is an emerging, multidrug-resistant fungal pathogen that has become a public health threat with an increasing incidence of infections worldwide. Candida auris spreads easily among patients within and between hospitals. Infections and outbreaks caused by C. auris have been reported in the Middle East region including Oman, Kuwait, Saudi Arabia, and Qatar; however, the origin of these isolates is largely unknown. Pathogen whole genome sequencing (WGS) was used to determine the epidemiology and drug resistance mutations of C. auris in Qatar. Forty-four samples isolated from patients in three hospitals and the hospital environment were sequenced by Illumina NextSeq. Core genome single nucleotide polymorphisms (SNPs) revealed that all isolates belonged to the South Asian lineage with genetic heterogeneity that suggests previous acquisition from foreign healthcare. The genetic variability among the outbreak isolates in the two hospitals (A and B) was low. Four environmental isolates clustered with the related clinical isolates, and epidemiologically linked isolates clustered together, suggesting that the ongoing transmission of C. auris could be linked to infected/colonized patients and the hospital environment. Prominent mutations Y132F and K143R in ERG11 linked to increased fluconazole resistance were detected. MDPI 2021-03-23 /pmc/articles/PMC8004815/ /pubmed/33807036 http://dx.doi.org/10.3390/jof7030240 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Salah, Husam
Sundararaju, Sathyavathi
Dalil, Lamya
Salameh, Sarah
Al-Wali, Walid
Tang, Patrick
Ben Abid, Fatma
Tsui, Clement K. M.
Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title_full Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title_fullStr Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title_full_unstemmed Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title_short Genomic Epidemiology of Candida auris in Qatar Reveals Hospital Transmission Dynamics and a South Asian Origin
title_sort genomic epidemiology of candida auris in qatar reveals hospital transmission dynamics and a south asian origin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004815/
https://www.ncbi.nlm.nih.gov/pubmed/33807036
http://dx.doi.org/10.3390/jof7030240
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