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Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry
High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004903/ https://www.ncbi.nlm.nih.gov/pubmed/33807031 http://dx.doi.org/10.3390/mi12030345 |
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author | Pärnamets, Kaiser Pardy, Tamas Koel, Ants Rang, Toomas Scheler, Ott Le Moullec, Yannick Afrin, Fariha |
author_facet | Pärnamets, Kaiser Pardy, Tamas Koel, Ants Rang, Toomas Scheler, Ott Le Moullec, Yannick Afrin, Fariha |
author_sort | Pärnamets, Kaiser |
collection | PubMed |
description | High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high flow rates, the droplets allow massive parallelization, further increasing the throughput of droplets. However, this novel methodology poses unique challenges related to commonly used fluorometry and fluorescent microscopy techniques. We review the optical sensor technology and light sources applicable to DMFC, as well as analyze the challenges and advantages of each option, primarily focusing on electronics. An analysis of low-cost and/or sufficiently compact systems that can be incorporated into portable devices is also presented. |
format | Online Article Text |
id | pubmed-8004903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80049032021-03-29 Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry Pärnamets, Kaiser Pardy, Tamas Koel, Ants Rang, Toomas Scheler, Ott Le Moullec, Yannick Afrin, Fariha Micromachines (Basel) Review High-throughput microflow cytometry has become a focal point of research in recent years. In particular, droplet microflow cytometry (DMFC) enables the analysis of cells reacting to different stimuli in chemical isolation due to each droplet acting as an isolated microreactor. Furthermore, at high flow rates, the droplets allow massive parallelization, further increasing the throughput of droplets. However, this novel methodology poses unique challenges related to commonly used fluorometry and fluorescent microscopy techniques. We review the optical sensor technology and light sources applicable to DMFC, as well as analyze the challenges and advantages of each option, primarily focusing on electronics. An analysis of low-cost and/or sufficiently compact systems that can be incorporated into portable devices is also presented. MDPI 2021-03-23 /pmc/articles/PMC8004903/ /pubmed/33807031 http://dx.doi.org/10.3390/mi12030345 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Review Pärnamets, Kaiser Pardy, Tamas Koel, Ants Rang, Toomas Scheler, Ott Le Moullec, Yannick Afrin, Fariha Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title | Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title_full | Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title_fullStr | Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title_full_unstemmed | Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title_short | Optical Detection Methods for High-Throughput Fluorescent Droplet Microflow Cytometry |
title_sort | optical detection methods for high-throughput fluorescent droplet microflow cytometry |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004903/ https://www.ncbi.nlm.nih.gov/pubmed/33807031 http://dx.doi.org/10.3390/mi12030345 |
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