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Affinity Purification of Angiotensin Converting Enzyme Inhibitory Peptides from Wakame (Undaria Pinnatifida) Using Immobilized ACE on Magnetic Metal Organic Frameworks

Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from marine organism have shown a blood pressure lowering effect with no side effects. A new affinity medium of Fe(3)O(4)@ZIF-90 immobilized ACE (Fe(3)O(4)@ZIF-90-ACE) was prepared and used in the purification of ACE inhibitory peptid...

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Detalles Bibliográficos
Autores principales: Feng, Xuezhen, Liao, Dankui, Sun, Lixia, Wu, Shanguang, Lan, Ping, Wang, Zefen, Li, Chunzhi, Zhou, Qian, Lu, Yuan, Lan, Xiongdiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004985/
https://www.ncbi.nlm.nih.gov/pubmed/33807119
http://dx.doi.org/10.3390/md19030177
Descripción
Sumario:Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from marine organism have shown a blood pressure lowering effect with no side effects. A new affinity medium of Fe(3)O(4)@ZIF-90 immobilized ACE (Fe(3)O(4)@ZIF-90-ACE) was prepared and used in the purification of ACE inhibitory peptides from Wakame (Undaria pinnatifida) protein hydrolysate (<5 kDa). The Fe(3)O(4)@ZIF-90 nanoparticles were prepared by a one-pot synthesis and crude ACE extract from pig lung was immobilized onto it, which exhibited excellent stability and reusability. A novel ACE inhibitory peptide, KNFL (inhibitory concentration 50, IC(50) = 225.87 μM) was identified by affinity purification using Fe(3)O(4)@ZIF-90-ACE combined with reverse phase-high performance liquid chromatography (RP-HPLC) and MALDI-TOF mass spectrometry. Lineweaver–Burk analysis confirmed the non-competitive inhibition pattern of KNFL, and molecular docking showed that it bound at a non-active site of ACE via hydrogen bonds. This demonstrates that affinity purification using Fe(3)O(4)@ZIF-90-ACE is a highly efficient method for separating ACE inhibitory peptides from complex protein mixtures and the purified peptide KNFL could be developed as a functional food ingredients against hypertension.