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The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract
The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of Ephedra aphylla as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and ta...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005055/ https://www.ncbi.nlm.nih.gov/pubmed/33809976 http://dx.doi.org/10.3390/biom11030470 |
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author | El-Zayat, Mustafa Mohsen Eraqi, Mostafa M. Alrefai, Hani El-Khateeb, Ayman Y. Ibrahim, Marwan A. Aljohani, Hashim M. Aljohani, Maher M. Elshaer, Moustafa Mohammed |
author_facet | El-Zayat, Mustafa Mohsen Eraqi, Mostafa M. Alrefai, Hani El-Khateeb, Ayman Y. Ibrahim, Marwan A. Aljohani, Hashim M. Aljohani, Maher M. Elshaer, Moustafa Mohammed |
author_sort | El-Zayat, Mustafa Mohsen |
collection | PubMed |
description | The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of Ephedra aphylla as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of Ephedra aphylla and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH(•) assay. The samples were assessed as anticancer agents against six tumor cells and a normal lung fibroblast (WI-38) cell line. The selenium nanoparticles of Ephedra aphylla extract revealed very strong cytotoxicity against HePG-2 cells (inhibitory concentration (IC(50)) = 7.56 ± 0.6 µg/mL), HCT-116 cells (IC(50) = 10.02 ± 0.9 µg/mL), and HeLa cells (IC(50) = 9.23 ± 0.8 µg/mL). The samples were evaluated as antimicrobial agents against bacterial and fungal strains. Thus, selenium nanoparticles showed potent activities against Gram-negative strains (Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli), Gram-positive strains (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, and Staphylococcus epidermidis), and the fungal strain Candida albicans. In conclusion, the preparation of nanoparticles of either selenium or zinc is crucial for improved biological characteristics. |
format | Online Article Text |
id | pubmed-8005055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80050552021-03-29 The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract El-Zayat, Mustafa Mohsen Eraqi, Mostafa M. Alrefai, Hani El-Khateeb, Ayman Y. Ibrahim, Marwan A. Aljohani, Hashim M. Aljohani, Maher M. Elshaer, Moustafa Mohammed Biomolecules Article The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of Ephedra aphylla as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of Ephedra aphylla and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH(•) assay. The samples were assessed as anticancer agents against six tumor cells and a normal lung fibroblast (WI-38) cell line. The selenium nanoparticles of Ephedra aphylla extract revealed very strong cytotoxicity against HePG-2 cells (inhibitory concentration (IC(50)) = 7.56 ± 0.6 µg/mL), HCT-116 cells (IC(50) = 10.02 ± 0.9 µg/mL), and HeLa cells (IC(50) = 9.23 ± 0.8 µg/mL). The samples were evaluated as antimicrobial agents against bacterial and fungal strains. Thus, selenium nanoparticles showed potent activities against Gram-negative strains (Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli), Gram-positive strains (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, and Staphylococcus epidermidis), and the fungal strain Candida albicans. In conclusion, the preparation of nanoparticles of either selenium or zinc is crucial for improved biological characteristics. MDPI 2021-03-22 /pmc/articles/PMC8005055/ /pubmed/33809976 http://dx.doi.org/10.3390/biom11030470 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article El-Zayat, Mustafa Mohsen Eraqi, Mostafa M. Alrefai, Hani El-Khateeb, Ayman Y. Ibrahim, Marwan A. Aljohani, Hashim M. Aljohani, Maher M. Elshaer, Moustafa Mohammed The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title_full | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title_fullStr | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title_full_unstemmed | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title_short | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using Ephedra aphylla Extract |
title_sort | antimicrobial, antioxidant, and anticancer activity of greenly synthesized selenium and zinc composite nanoparticles using ephedra aphylla extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005055/ https://www.ncbi.nlm.nih.gov/pubmed/33809976 http://dx.doi.org/10.3390/biom11030470 |
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