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Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect

Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are...

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Autores principales: Stolyar, Sergey V., Kolenchukova, Oksana A., Boldyreva, Anna V., Kudryasheva, Nadezda S., Gerasimova, Yulia V., Krasikov, Alexandr A., Yaroslavtsev, Roman N., Bayukov, Oleg A., Ladygina, Valentina P., Birukova, Elena A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005073/
https://www.ncbi.nlm.nih.gov/pubmed/33810151
http://dx.doi.org/10.3390/biomedicines9030323
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author Stolyar, Sergey V.
Kolenchukova, Oksana A.
Boldyreva, Anna V.
Kudryasheva, Nadezda S.
Gerasimova, Yulia V.
Krasikov, Alexandr A.
Yaroslavtsev, Roman N.
Bayukov, Oleg A.
Ladygina, Valentina P.
Birukova, Elena A.
author_facet Stolyar, Sergey V.
Kolenchukova, Oksana A.
Boldyreva, Anna V.
Kudryasheva, Nadezda S.
Gerasimova, Yulia V.
Krasikov, Alexandr A.
Yaroslavtsev, Roman N.
Bayukov, Oleg A.
Ladygina, Valentina P.
Birukova, Elena A.
author_sort Stolyar, Sergey V.
collection PubMed
description Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are superparamagnetic at room temperature, and the characteristic blocking temperature is 23–25 K. The uncompensated moment of ferrihydrite particles was determined to be approximately 200 Bohr magnetons. In vitro testing of different concentrations of ferrihydrite nanoparticles for the functional activity of neutrophilic granulocytes by the chemiluminescence method showed an increase in the release of primary oxygen radicals by blood phagocytes when exposed to a minimum concentration and a decrease in secondary radicals when exposed to a maximum concentration. In vivo testing of ferrihydrite nanoparticles on Wister rats showed that a suspension of ferrihydrite nanoparticles has chronic toxicity, since it causes morphological changes in organs, mainly in the spleen, which are characterized by the accumulation of hemosiderin nanoparticles (stained blue according to Perls). Ferrihydrite can also directly or indirectly stimulate the proliferation and intracellular regeneration of hepatocytes. The partial detection of Perls-positive cells in the liver and kidneys can be explained by the rapid elimination from organs and the high dispersion of the nanomaterial. Thus, it is necessary to carry out studies of these processes at the systemic level, since the introduction of nanoparticles into the body is characterized by adaptive-proliferative processes, accompanied by the development of cell dystrophy and tension of the phagocytic system.
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spelling pubmed-80050732021-03-29 Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect Stolyar, Sergey V. Kolenchukova, Oksana A. Boldyreva, Anna V. Kudryasheva, Nadezda S. Gerasimova, Yulia V. Krasikov, Alexandr A. Yaroslavtsev, Roman N. Bayukov, Oleg A. Ladygina, Valentina P. Birukova, Elena A. Biomedicines Article Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are superparamagnetic at room temperature, and the characteristic blocking temperature is 23–25 K. The uncompensated moment of ferrihydrite particles was determined to be approximately 200 Bohr magnetons. In vitro testing of different concentrations of ferrihydrite nanoparticles for the functional activity of neutrophilic granulocytes by the chemiluminescence method showed an increase in the release of primary oxygen radicals by blood phagocytes when exposed to a minimum concentration and a decrease in secondary radicals when exposed to a maximum concentration. In vivo testing of ferrihydrite nanoparticles on Wister rats showed that a suspension of ferrihydrite nanoparticles has chronic toxicity, since it causes morphological changes in organs, mainly in the spleen, which are characterized by the accumulation of hemosiderin nanoparticles (stained blue according to Perls). Ferrihydrite can also directly or indirectly stimulate the proliferation and intracellular regeneration of hepatocytes. The partial detection of Perls-positive cells in the liver and kidneys can be explained by the rapid elimination from organs and the high dispersion of the nanomaterial. Thus, it is necessary to carry out studies of these processes at the systemic level, since the introduction of nanoparticles into the body is characterized by adaptive-proliferative processes, accompanied by the development of cell dystrophy and tension of the phagocytic system. MDPI 2021-03-22 /pmc/articles/PMC8005073/ /pubmed/33810151 http://dx.doi.org/10.3390/biomedicines9030323 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Stolyar, Sergey V.
Kolenchukova, Oksana A.
Boldyreva, Anna V.
Kudryasheva, Nadezda S.
Gerasimova, Yulia V.
Krasikov, Alexandr A.
Yaroslavtsev, Roman N.
Bayukov, Oleg A.
Ladygina, Valentina P.
Birukova, Elena A.
Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title_full Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title_fullStr Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title_full_unstemmed Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title_short Biogenic Ferrihydrite Nanoparticles: Synthesis, Properties In Vitro and In Vivo Testing and the Concentration Effect
title_sort biogenic ferrihydrite nanoparticles: synthesis, properties in vitro and in vivo testing and the concentration effect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005073/
https://www.ncbi.nlm.nih.gov/pubmed/33810151
http://dx.doi.org/10.3390/biomedicines9030323
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