Cargando…
Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4
BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is one of the best-characterized and most pervasive renal cancers. The present study aimed to explore the effects and potential mechanisms of let-7i-5p in ccRCC cells. METHODS: Using bioinformatics analyses, we investigated the expression of let-7i...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005230/ https://www.ncbi.nlm.nih.gov/pubmed/33775245 http://dx.doi.org/10.1186/s12894-021-00820-9 |
_version_ | 1783672086251700224 |
---|---|
author | Liu, Yujie Hu, Xing Hu, Liang Xu, Changjing Liang, Xuemei |
author_facet | Liu, Yujie Hu, Xing Hu, Liang Xu, Changjing Liang, Xuemei |
author_sort | Liu, Yujie |
collection | PubMed |
description | BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is one of the best-characterized and most pervasive renal cancers. The present study aimed to explore the effects and potential mechanisms of let-7i-5p in ccRCC cells. METHODS: Using bioinformatics analyses, we investigated the expression of let-7i-5p in The Cancer Genome Atlas (TCGA) database and predicted biological functions and possible target genes of let-7i-5p in ccRCC cells. Cell proliferation assay, wound healing assay and transwell invasion assay were conducted to characterize the effects of let-7i-5p in ccRCC cells. To verify the interactions between let-7i-5p and HABP4, dual-luciferase reporter assay, quantitative real-time polymerase chain reaction, and western blotting were conducted. Rescue experiments were used to investigate the relationship between let-7i-5p and HABP4. RESULTS: TCGA data analysis revealed that ccRCC tissues had significantly increased let-7i-5p expression, which was robustly associated with poor overall survival. Further verification showed that ccRCC cell proliferation, migration and invasion were inhibited by let-7i-5p inhibitor but enhanced by let-7i-5p mimics. Subsequently, HABP4 was predicted to be the target gene of let-7i-5p. TCGA data showed that ccRCC tissues had decreased expression of HABP4 and that HABP4 expression was negatively correlated with let-7i-5p. Further verification showed that downregulation of HABP4 expression promoted cell proliferation, migration and invasion. The dual-luciferase reporter gene assay suggested that the let-7i-5p/HABP4 axis was responsible for the development of ccRCC. CONCLUSION: Our results provide evidence that let-7i-5p functions as a tumor promoter in ccRCC and facilitates cell proliferation, migration and invasion by targeting HABP4. These results clarify the pathogenesis of ccRCC and offer a potential target for its treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-021-00820-9. |
format | Online Article Text |
id | pubmed-8005230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80052302021-03-30 Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 Liu, Yujie Hu, Xing Hu, Liang Xu, Changjing Liang, Xuemei BMC Urol Research Article BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is one of the best-characterized and most pervasive renal cancers. The present study aimed to explore the effects and potential mechanisms of let-7i-5p in ccRCC cells. METHODS: Using bioinformatics analyses, we investigated the expression of let-7i-5p in The Cancer Genome Atlas (TCGA) database and predicted biological functions and possible target genes of let-7i-5p in ccRCC cells. Cell proliferation assay, wound healing assay and transwell invasion assay were conducted to characterize the effects of let-7i-5p in ccRCC cells. To verify the interactions between let-7i-5p and HABP4, dual-luciferase reporter assay, quantitative real-time polymerase chain reaction, and western blotting were conducted. Rescue experiments were used to investigate the relationship between let-7i-5p and HABP4. RESULTS: TCGA data analysis revealed that ccRCC tissues had significantly increased let-7i-5p expression, which was robustly associated with poor overall survival. Further verification showed that ccRCC cell proliferation, migration and invasion were inhibited by let-7i-5p inhibitor but enhanced by let-7i-5p mimics. Subsequently, HABP4 was predicted to be the target gene of let-7i-5p. TCGA data showed that ccRCC tissues had decreased expression of HABP4 and that HABP4 expression was negatively correlated with let-7i-5p. Further verification showed that downregulation of HABP4 expression promoted cell proliferation, migration and invasion. The dual-luciferase reporter gene assay suggested that the let-7i-5p/HABP4 axis was responsible for the development of ccRCC. CONCLUSION: Our results provide evidence that let-7i-5p functions as a tumor promoter in ccRCC and facilitates cell proliferation, migration and invasion by targeting HABP4. These results clarify the pathogenesis of ccRCC and offer a potential target for its treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-021-00820-9. BioMed Central 2021-03-28 /pmc/articles/PMC8005230/ /pubmed/33775245 http://dx.doi.org/10.1186/s12894-021-00820-9 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Liu, Yujie Hu, Xing Hu, Liang Xu, Changjing Liang, Xuemei Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title | Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title_full | Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title_fullStr | Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title_full_unstemmed | Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title_short | Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4 |
title_sort | let-7i-5p enhances cell proliferation, migration and invasion of ccrcc by targeting habp4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005230/ https://www.ncbi.nlm.nih.gov/pubmed/33775245 http://dx.doi.org/10.1186/s12894-021-00820-9 |
work_keys_str_mv | AT liuyujie let7i5penhancescellproliferationmigrationandinvasionofccrccbytargetinghabp4 AT huxing let7i5penhancescellproliferationmigrationandinvasionofccrccbytargetinghabp4 AT huliang let7i5penhancescellproliferationmigrationandinvasionofccrccbytargetinghabp4 AT xuchangjing let7i5penhancescellproliferationmigrationandinvasionofccrccbytargetinghabp4 AT liangxuemei let7i5penhancescellproliferationmigrationandinvasionofccrccbytargetinghabp4 |