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Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium

Pterygium is a common ocular surface disease characterized by abnormal fibrovascular proliferation and invasion, similar to tumorigenesis. The formation of tumors is related to a change in the expression of various RNAs; however, whether they are involved in the formation and development of pterygiu...

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Autores principales: Liu, Xin, Zhang, Jing, Nie, Danyao, Zeng, Kun, Hu, Huiling, Tie, Jinjun, Sun, Liangnan, Peng, Ling, Liu, Xinhua, Wang, Jiantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005612/
https://www.ncbi.nlm.nih.gov/pubmed/33790949
http://dx.doi.org/10.3389/fgene.2021.646550
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author Liu, Xin
Zhang, Jing
Nie, Danyao
Zeng, Kun
Hu, Huiling
Tie, Jinjun
Sun, Liangnan
Peng, Ling
Liu, Xinhua
Wang, Jiantao
author_facet Liu, Xin
Zhang, Jing
Nie, Danyao
Zeng, Kun
Hu, Huiling
Tie, Jinjun
Sun, Liangnan
Peng, Ling
Liu, Xinhua
Wang, Jiantao
author_sort Liu, Xin
collection PubMed
description Pterygium is a common ocular surface disease characterized by abnormal fibrovascular proliferation and invasion, similar to tumorigenesis. The formation of tumors is related to a change in the expression of various RNAs; however, whether they are involved in the formation and development of pterygium remains unclear. In this study, transcriptome analysis of messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) of paired pterygium and normal conjunctiva was performed to explore key genes regulating the development of pterygium. In total, 579 mRNAs, 275 lncRNAs, and 21 circRNAs were differentially expressed (DE) in pterygium compared with paired conjunctival tissues. Functional enrichment analysis indicated that DE RNAs were associated with extracellular matrix organization, blood vessel morphogenesis, and focal adhesion. Furthermore, through protein-protein interaction network and mRNA-lncRNA co-expression network analysis, key mRNAs including FN1, VCAM1, and MMP2, and key lncRNAs including MIR4435-2HG and LINC00968 were screened and might be involved in the pathogenesis of pterygium. In addition, several circRNAs including hsa_circ_0007482 and hsa_circ_001730 were considered to be involved in the pterygium development. This study provides a scientific basis for elucidating the pathogenesis of pterygium and will be beneficial for the development of preventive and therapeutic strategies.
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spelling pubmed-80056122021-03-30 Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium Liu, Xin Zhang, Jing Nie, Danyao Zeng, Kun Hu, Huiling Tie, Jinjun Sun, Liangnan Peng, Ling Liu, Xinhua Wang, Jiantao Front Genet Genetics Pterygium is a common ocular surface disease characterized by abnormal fibrovascular proliferation and invasion, similar to tumorigenesis. The formation of tumors is related to a change in the expression of various RNAs; however, whether they are involved in the formation and development of pterygium remains unclear. In this study, transcriptome analysis of messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) of paired pterygium and normal conjunctiva was performed to explore key genes regulating the development of pterygium. In total, 579 mRNAs, 275 lncRNAs, and 21 circRNAs were differentially expressed (DE) in pterygium compared with paired conjunctival tissues. Functional enrichment analysis indicated that DE RNAs were associated with extracellular matrix organization, blood vessel morphogenesis, and focal adhesion. Furthermore, through protein-protein interaction network and mRNA-lncRNA co-expression network analysis, key mRNAs including FN1, VCAM1, and MMP2, and key lncRNAs including MIR4435-2HG and LINC00968 were screened and might be involved in the pathogenesis of pterygium. In addition, several circRNAs including hsa_circ_0007482 and hsa_circ_001730 were considered to be involved in the pterygium development. This study provides a scientific basis for elucidating the pathogenesis of pterygium and will be beneficial for the development of preventive and therapeutic strategies. Frontiers Media S.A. 2021-03-15 /pmc/articles/PMC8005612/ /pubmed/33790949 http://dx.doi.org/10.3389/fgene.2021.646550 Text en Copyright © 2021 Liu, Zhang, Nie, Zeng, Hu, Tie, Sun, Peng, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Liu, Xin
Zhang, Jing
Nie, Danyao
Zeng, Kun
Hu, Huiling
Tie, Jinjun
Sun, Liangnan
Peng, Ling
Liu, Xinhua
Wang, Jiantao
Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title_full Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title_fullStr Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title_full_unstemmed Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title_short Comparative Transcriptomic Analysis to Identify the Important Coding and Non-coding RNAs Involved in the Pathogenesis of Pterygium
title_sort comparative transcriptomic analysis to identify the important coding and non-coding rnas involved in the pathogenesis of pterygium
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005612/
https://www.ncbi.nlm.nih.gov/pubmed/33790949
http://dx.doi.org/10.3389/fgene.2021.646550
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