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Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice

We transduced mouse cortical astrocytes cultured from four litters of embryonic wildtype (WT) and connexin43 (Cx43) null mouse pups with lentiviral vector encoding hTERT and measured expression of astrocyte-specific markers up to passage 10 (p10). The immortalized cell lines thus generated (designat...

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Detalles Bibliográficos
Autores principales: Cibelli, Antonio, Veronica Lopez-Quintero, Sandra, Mccutcheon, Sean, Scemes, Eliana, Spray, David C., Stout, Randy F., Suadicani, Sylvia O., Thi, Mia M., Urban-Maldonado, Marcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005635/
https://www.ncbi.nlm.nih.gov/pubmed/33790744
http://dx.doi.org/10.3389/fncel.2021.647109

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