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Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans
OBJECTIVES: The comprehensive detection of environmental chemicals in biospecimens, an indispensable task in exposome research, is advancing. This study aimed to develop an exposomic approach to identify urinary metabolites of organophosphate (OP) pesticides, specifically cadusafos and prothiofos me...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005856/ https://www.ncbi.nlm.nih.gov/pubmed/33779022 http://dx.doi.org/10.1002/1348-9585.12218 |
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author | Nomasa, Karin Oya, Naoko Ito, Yuki Terajima, Takehito Nishino, Takahiro Mohanto, Nayan Chandra Sato, Hirotaka Tomizawa, Motohiro Kamijima, Michihiro |
author_facet | Nomasa, Karin Oya, Naoko Ito, Yuki Terajima, Takehito Nishino, Takahiro Mohanto, Nayan Chandra Sato, Hirotaka Tomizawa, Motohiro Kamijima, Michihiro |
author_sort | Nomasa, Karin |
collection | PubMed |
description | OBJECTIVES: The comprehensive detection of environmental chemicals in biospecimens, an indispensable task in exposome research, is advancing. This study aimed to develop an exposomic approach to identify urinary metabolites of organophosphate (OP) pesticides, specifically cadusafos and prothiofos metabolites, as an example chemical group, using an original metabolome dataset generated from animal experiments. METHODS: Urine samples from 73 university students were analyzed using liquid chromatography–high‐resolution mass spectrometry. The metabolome data, including the exact masses, retention time (t(R)), and tandem mass spectra obtained from the human samples, were compared with the existing reference databases and with our original metabolome dataset for cadusafos and prothiofos, which was produced from mice to whom two doses of these OPs were orally administered. RESULTS: Using the existing databases, one chromatographic peak was annotated as 2,4‐dichlorophenol, which could be a prothiofos metabolite. Using our original dataset, one peak was annotated as a putative cadusafos metabolite and three peaks as putative prothiofos metabolites. Of these, all three peaks suggestive of prothiofos metabolites, 2,4‐dichlorophenol, 3,4,5‐trihydroxy‐6‐(2,4‐dichlorophenoxy) oxane‐2‐carboxylic acid, and (2,4‐dichlorophenyl) hydrogen sulfate were confirmed as authentic compounds by comparing their peak data with both the original dataset and peak data of the standard reagents. The putative cadusafos metabolite was identified as a level C compound (metabolite candidate with limited plausibility). CONCLUSIONS: Our developed method successfully identified prothiofos metabolites that are usually not a target of biomonitoring studies. Our approach is extensively applicable to various environmental contaminants beyond OP pesticides. |
format | Online Article Text |
id | pubmed-8005856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80058562021-04-01 Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans Nomasa, Karin Oya, Naoko Ito, Yuki Terajima, Takehito Nishino, Takahiro Mohanto, Nayan Chandra Sato, Hirotaka Tomizawa, Motohiro Kamijima, Michihiro J Occup Health Original Articles OBJECTIVES: The comprehensive detection of environmental chemicals in biospecimens, an indispensable task in exposome research, is advancing. This study aimed to develop an exposomic approach to identify urinary metabolites of organophosphate (OP) pesticides, specifically cadusafos and prothiofos metabolites, as an example chemical group, using an original metabolome dataset generated from animal experiments. METHODS: Urine samples from 73 university students were analyzed using liquid chromatography–high‐resolution mass spectrometry. The metabolome data, including the exact masses, retention time (t(R)), and tandem mass spectra obtained from the human samples, were compared with the existing reference databases and with our original metabolome dataset for cadusafos and prothiofos, which was produced from mice to whom two doses of these OPs were orally administered. RESULTS: Using the existing databases, one chromatographic peak was annotated as 2,4‐dichlorophenol, which could be a prothiofos metabolite. Using our original dataset, one peak was annotated as a putative cadusafos metabolite and three peaks as putative prothiofos metabolites. Of these, all three peaks suggestive of prothiofos metabolites, 2,4‐dichlorophenol, 3,4,5‐trihydroxy‐6‐(2,4‐dichlorophenoxy) oxane‐2‐carboxylic acid, and (2,4‐dichlorophenyl) hydrogen sulfate were confirmed as authentic compounds by comparing their peak data with both the original dataset and peak data of the standard reagents. The putative cadusafos metabolite was identified as a level C compound (metabolite candidate with limited plausibility). CONCLUSIONS: Our developed method successfully identified prothiofos metabolites that are usually not a target of biomonitoring studies. Our approach is extensively applicable to various environmental contaminants beyond OP pesticides. John Wiley and Sons Inc. 2021-03-29 /pmc/articles/PMC8005856/ /pubmed/33779022 http://dx.doi.org/10.1002/1348-9585.12218 Text en © 2021 The Authors. Journal of Occupational Health published by John Wiley & Sons Australia, Ltd on behalf of The Japan Society for Occupational Health This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Nomasa, Karin Oya, Naoko Ito, Yuki Terajima, Takehito Nishino, Takahiro Mohanto, Nayan Chandra Sato, Hirotaka Tomizawa, Motohiro Kamijima, Michihiro Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title | Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title_full | Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title_fullStr | Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title_full_unstemmed | Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title_short | Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
title_sort | development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: extrapolation of cadusafos and prothiofos metabolomics data of mice to humans |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005856/ https://www.ncbi.nlm.nih.gov/pubmed/33779022 http://dx.doi.org/10.1002/1348-9585.12218 |
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