Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experim...

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Autores principales: Li, Weifeng, Fan, Zhenlin, Lin, Yan, Wang, Tian-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006267/
https://www.ncbi.nlm.nih.gov/pubmed/33791287
http://dx.doi.org/10.3389/fbioe.2021.646363
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author Li, Weifeng
Fan, Zhenlin
Lin, Yan
Wang, Tian-Yun
author_facet Li, Weifeng
Fan, Zhenlin
Lin, Yan
Wang, Tian-Yun
author_sort Li, Weifeng
collection PubMed
description At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.
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spelling pubmed-80062672021-03-30 Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells Li, Weifeng Fan, Zhenlin Lin, Yan Wang, Tian-Yun Front Bioeng Biotechnol Bioengineering and Biotechnology At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed. Frontiers Media S.A. 2021-03-15 /pmc/articles/PMC8006267/ /pubmed/33791287 http://dx.doi.org/10.3389/fbioe.2021.646363 Text en Copyright © 2021 Li, Fan, Lin and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Weifeng
Fan, Zhenlin
Lin, Yan
Wang, Tian-Yun
Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title_full Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title_fullStr Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title_full_unstemmed Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title_short Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
title_sort serum-free medium for recombinant protein expression in chinese hamster ovary cells
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006267/
https://www.ncbi.nlm.nih.gov/pubmed/33791287
http://dx.doi.org/10.3389/fbioe.2021.646363
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AT wangtianyun serumfreemediumforrecombinantproteinexpressioninchinesehamsterovarycells

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