Cargando…

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experim...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Weifeng, Fan, Zhenlin, Lin, Yan, Wang, Tian-Yun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006267/ https://www.ncbi.nlm.nih.gov/pubmed/33791287 http://dx.doi.org/10.3389/fbioe.2021.646363

Ejemplares similares

Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
por: Li, Zheng-Mei, et al.
Publicado: (2022)

Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells
por: Zhang, Huan-Yu, et al.
Publicado: (2021)

Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells
por: Zhang, Jun-He, et al.
Publicado: (2022)

Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones
por: Yang, Wenwen, et al.
Publicado: (2022)

The Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells
por: Becker, Max, et al.
Publicado: (2019)

Enhancing and stabilizing monoclonal antibody production by Chinese hamster ovary (CHO) cells with optimized perfusion culture strategies
por: Liang, Kexue, et al.
Publicado: (2023)

Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks
por: Ihling, Nina, et al.
Publicado: (2021)

Biopharmaceutical-Type Chinese Hamster Ovary Cell Cultivation Under Static Magnetic Field Exposure: A Study of Genotoxic Effect
por: Rekena, Alina, et al.
Publicado: (2021)

Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells
por: Min, Honggi, et al.
Publicado: (2022)

Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein Expression
por: Budge, James D., et al.
Publicado: (2021)

Serum-Free Medium Enhances the Therapeutic Effects of Umbilical Cord Mesenchymal Stromal Cells on a Murine Model for Acute Colitis
por: Wu, Xiaoyun, et al.
Publicado: (2020)

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System
por: Park, Yu-Jin, et al.
Publicado: (2021)

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells
por: Wei, Mian, et al.
Publicado: (2022)

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
por: Liu, Hui-Ning, et al.
Publicado: (2022)

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
por: Tripathi, Nagesh K., et al.
Publicado: (2019)

Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P(DH) promoter
por: Bernat-Camps, Núria, et al.
Publicado: (2023)

The Therapeutic Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cell Conditioned Medium on Experimental Colitis Was Improved by the Serum From Colitis Rats
por: Qi, Li-li, et al.
Publicado: (2021)

Corrigendum: The therapeutic efficacy of adipose tissue-derived mesenchymal stem cell conditioned medium on experimental colitis was improved by the serum from colitis rats
por: Qi, Li-li, et al.
Publicado: (2022)

Cell-Free Glycoengineering of the Recombinant SARS-CoV-2 Spike Glycoprotein
por: Ruhnau, Johannes, et al.
Publicado: (2021)

Microcarrier Screening and Evaluation for Dynamic Expansion of Human Periosteum-Derived Progenitor Cells in a Xenogeneic Free Medium
por: Van Beylen, Kathleen, et al.
Publicado: (2021)

A positive Selection Escherichia Coli Recombinant Protein Expression Vector for One-Step Cloning
por: James, Shinto, et al.
Publicado: (2022)

Environment Control to Improve Recombinant Protein Yields in Plants Based on Agrobacterium-Mediated Transient Gene Expression
por: Fujiuchi, Naomichi, et al.
Publicado: (2016)

FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells
por: Coker, Jesse A., et al.
Publicado: (2022)

The Secretome Derived From Mesenchymal Stromal Cells Cultured in a Xeno-Free Medium Promotes Human Cartilage Recovery in vitro
por: Palamà, Maria Elisabetta Federica, et al.
Publicado: (2020)

The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles
por: Jang, Mi, et al.
Publicado: (2022)

The Promoter Toolbox for Recombinant Gene Expression in Trichoderma reesei
por: Fitz, Elisabeth, et al.
Publicado: (2018)

Conditional protein degradation in Yarrowia lipolytica using the auxin-inducible degron
por: Han, Zhenlin, et al.
Publicado: (2023)

Effects of fermentation medium on cigar filler
por: Wen, Cai, et al.
Publicado: (2022)

A Chemically Defined, Xeno- and Blood-Free Culture Medium Sustains Increased Production of Small Extracellular Vesicles From Mesenchymal Stem Cells
por: Figueroa-Valdés, Aliosha I., et al.
Publicado: (2021)

Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production
por: Manfrão-Netto, João Heitor Colombelli, et al.
Publicado: (2019)

Promoter from Chinese hamster elongation factor-1a gene and Epstein-Barr virus terminal repeats concatemer fragment maintain stable high-level expression of recombinant proteins
por: Sinegubova, Maria V., et al.
Publicado: (2023)

Effects of adding cocoa fermentation medium on cigar leaves in agricultural fermentation stage
por: Zong, Pengfei, et al.
Publicado: (2023)

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression
por: Ng, Jian Yao, et al.
Publicado: (2020)

Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery
por: Stoffels, Laura, et al.
Publicado: (2019)

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
por: Karyolaimos, Alexandros, et al.
Publicado: (2021)

Comparison of the Unfolded Protein Response in Cellobiose Utilization of Recombinant Angel- and W303-1A-Derived Yeast Expressing β-Glucosidase
por: Zou, Shaolan, et al.
Publicado: (2022)

Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells
por: Ho, Steven C. L., et al.
Publicado: (2016)

Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production
por: Karyolaimos, Alexandros, et al.
Publicado: (2020)

Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase
por: Karamitros, Christos S., et al.
Publicado: (2021)

Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation
por: Korntner, Stefanie H., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_mdatjankbvpgtgekrsq66qjlq5