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Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters

CO(2) fermentation by biocatalysis is a promising route for the sustainable production of valuable chemicals and fuels, such as acetic acid and ethanol. Considering the important role of environmental parameters on fermentation processes, granular sludge from an industrial anaerobic wastewater treat...

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Autores principales: He, Yaxue, Cassarini, Chiara, Lens, Piet N. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006448/
https://www.ncbi.nlm.nih.gov/pubmed/33790882
http://dx.doi.org/10.3389/fmicb.2021.647370
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author He, Yaxue
Cassarini, Chiara
Lens, Piet N. L.
author_facet He, Yaxue
Cassarini, Chiara
Lens, Piet N. L.
author_sort He, Yaxue
collection PubMed
description CO(2) fermentation by biocatalysis is a promising route for the sustainable production of valuable chemicals and fuels, such as acetic acid and ethanol. Considering the important role of environmental parameters on fermentation processes, granular sludge from an industrial anaerobic wastewater treatment system was tested as inoculum for ethanol production from H(2)/CO(2) at psychrophilic (18°C), submesophilic (25°C), and mesophilic (30°C) temperatures. The highest acetic acid and ethanol production was obtained at 25°C with a final concentration of 29.7 and 8.8 mM, respectively. The presence of bicarbonate enhanced acetic acid production 3.0 ∼ 4.1-fold, while inhibiting ethanol production. The addition of 0.3 g/L glucose induced butyric acid production (3.7 mM), while 5.7 mM ethanol was produced at the end of the incubation at pH 4 with glucose. The addition of 10 μM W enhanced ethanol production up to 3.8 and 7.0-fold compared to, respectively, 2 μM W addition and the control. The addition of 2 μM Mo enhanced ethanol production up to 8.1- and 5.4-fold compared to, respectively, 10 μM Mo and the control. This study showed that ethanol production from H(2)/CO(2) conversion using granular sludge as the inoculum can be optimized by selecting the operational temperature and by trace metal addition.
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spelling pubmed-80064482021-03-30 Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters He, Yaxue Cassarini, Chiara Lens, Piet N. L. Front Microbiol Microbiology CO(2) fermentation by biocatalysis is a promising route for the sustainable production of valuable chemicals and fuels, such as acetic acid and ethanol. Considering the important role of environmental parameters on fermentation processes, granular sludge from an industrial anaerobic wastewater treatment system was tested as inoculum for ethanol production from H(2)/CO(2) at psychrophilic (18°C), submesophilic (25°C), and mesophilic (30°C) temperatures. The highest acetic acid and ethanol production was obtained at 25°C with a final concentration of 29.7 and 8.8 mM, respectively. The presence of bicarbonate enhanced acetic acid production 3.0 ∼ 4.1-fold, while inhibiting ethanol production. The addition of 0.3 g/L glucose induced butyric acid production (3.7 mM), while 5.7 mM ethanol was produced at the end of the incubation at pH 4 with glucose. The addition of 10 μM W enhanced ethanol production up to 3.8 and 7.0-fold compared to, respectively, 2 μM W addition and the control. The addition of 2 μM Mo enhanced ethanol production up to 8.1- and 5.4-fold compared to, respectively, 10 μM Mo and the control. This study showed that ethanol production from H(2)/CO(2) conversion using granular sludge as the inoculum can be optimized by selecting the operational temperature and by trace metal addition. Frontiers Media S.A. 2021-03-10 /pmc/articles/PMC8006448/ /pubmed/33790882 http://dx.doi.org/10.3389/fmicb.2021.647370 Text en Copyright © 2021 He, Cassarini and Lens. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Yaxue
Cassarini, Chiara
Lens, Piet N. L.
Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title_full Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title_fullStr Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title_full_unstemmed Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title_short Bioethanol Production From H(2)/CO(2) by Solventogenesis Using Anaerobic Granular Sludge: Effect of Process Parameters
title_sort bioethanol production from h(2)/co(2) by solventogenesis using anaerobic granular sludge: effect of process parameters
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006448/
https://www.ncbi.nlm.nih.gov/pubmed/33790882
http://dx.doi.org/10.3389/fmicb.2021.647370
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