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Current Advances in DNA Methylation Analysis Methods

DNA methylation is one of the epigenetic changes, which plays a major role in regulating gene expression and, thus, many biological processes and diseases. There are several methods for determining the methylation of DNA samples. However, selecting the most appropriate method for answering biologica...

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Autores principales: Khodadadi, Ehsan, Fahmideh, Leila, Khodadadi, Ehsaneh, Dao, Sounkalo, Yousefi, Mehdi, Taghizadeh, Sepehr, Asgharzadeh, Mohammad, Yousefi, Bahman, Kafil, Hossein Samadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007345/
https://www.ncbi.nlm.nih.gov/pubmed/33824878
http://dx.doi.org/10.1155/2021/8827516
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author Khodadadi, Ehsan
Fahmideh, Leila
Khodadadi, Ehsaneh
Dao, Sounkalo
Yousefi, Mehdi
Taghizadeh, Sepehr
Asgharzadeh, Mohammad
Yousefi, Bahman
Kafil, Hossein Samadi
author_facet Khodadadi, Ehsan
Fahmideh, Leila
Khodadadi, Ehsaneh
Dao, Sounkalo
Yousefi, Mehdi
Taghizadeh, Sepehr
Asgharzadeh, Mohammad
Yousefi, Bahman
Kafil, Hossein Samadi
author_sort Khodadadi, Ehsan
collection PubMed
description DNA methylation is one of the epigenetic changes, which plays a major role in regulating gene expression and, thus, many biological processes and diseases. There are several methods for determining the methylation of DNA samples. However, selecting the most appropriate method for answering biological questions appears to be a challenging task. The primary methods in DNA methylation focused on identifying the state of methylation of the examined genes and determining the total amount of 5-methyl cytosine. The study of DNA methylation at a large scale of genomic levels became possible following the use of microarray hybridization technology. The new generation of sequencing platforms now allows the preparation of genomic maps of DNA methylation at the single-open level. This review includes the majority of methods available to date, introducing the most widely used methods, the bisulfite treatment, biological identification, and chemical cutting along with their advantages and disadvantages. The techniques are then scrutinized according to their robustness, high throughput capabilities, and cost.
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spelling pubmed-80073452021-04-05 Current Advances in DNA Methylation Analysis Methods Khodadadi, Ehsan Fahmideh, Leila Khodadadi, Ehsaneh Dao, Sounkalo Yousefi, Mehdi Taghizadeh, Sepehr Asgharzadeh, Mohammad Yousefi, Bahman Kafil, Hossein Samadi Biomed Res Int Review Article DNA methylation is one of the epigenetic changes, which plays a major role in regulating gene expression and, thus, many biological processes and diseases. There are several methods for determining the methylation of DNA samples. However, selecting the most appropriate method for answering biological questions appears to be a challenging task. The primary methods in DNA methylation focused on identifying the state of methylation of the examined genes and determining the total amount of 5-methyl cytosine. The study of DNA methylation at a large scale of genomic levels became possible following the use of microarray hybridization technology. The new generation of sequencing platforms now allows the preparation of genomic maps of DNA methylation at the single-open level. This review includes the majority of methods available to date, introducing the most widely used methods, the bisulfite treatment, biological identification, and chemical cutting along with their advantages and disadvantages. The techniques are then scrutinized according to their robustness, high throughput capabilities, and cost. Hindawi 2021-03-20 /pmc/articles/PMC8007345/ /pubmed/33824878 http://dx.doi.org/10.1155/2021/8827516 Text en Copyright © 2021 Ehsan Khodadadi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Khodadadi, Ehsan
Fahmideh, Leila
Khodadadi, Ehsaneh
Dao, Sounkalo
Yousefi, Mehdi
Taghizadeh, Sepehr
Asgharzadeh, Mohammad
Yousefi, Bahman
Kafil, Hossein Samadi
Current Advances in DNA Methylation Analysis Methods
title Current Advances in DNA Methylation Analysis Methods
title_full Current Advances in DNA Methylation Analysis Methods
title_fullStr Current Advances in DNA Methylation Analysis Methods
title_full_unstemmed Current Advances in DNA Methylation Analysis Methods
title_short Current Advances in DNA Methylation Analysis Methods
title_sort current advances in dna methylation analysis methods
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007345/
https://www.ncbi.nlm.nih.gov/pubmed/33824878
http://dx.doi.org/10.1155/2021/8827516
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