Cargando…
Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil
ABSTRACT: Lignin bio-valorization is an emerging field of applied biotechnology and has not yet been studied at low temperatures. Paraburkholderia aromaticivorans AR20-38 was examined for its potential to degrade six selected lignin monomers (syringic acid, p-coumaric acid, 4-hydroxybenzoic acid, fe...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007519/ https://www.ncbi.nlm.nih.gov/pubmed/33687503 http://dx.doi.org/10.1007/s00253-021-11215-z |
_version_ | 1783672506616381440 |
---|---|
author | Margesin, Rosa Volgger, Georg Wagner, Andreas O. Zhang, Dechao Poyntner, Caroline |
author_facet | Margesin, Rosa Volgger, Georg Wagner, Andreas O. Zhang, Dechao Poyntner, Caroline |
author_sort | Margesin, Rosa |
collection | PubMed |
description | ABSTRACT: Lignin bio-valorization is an emerging field of applied biotechnology and has not yet been studied at low temperatures. Paraburkholderia aromaticivorans AR20-38 was examined for its potential to degrade six selected lignin monomers (syringic acid, p-coumaric acid, 4-hydroxybenzoic acid, ferulic acid, vanillic acid, benzoic acid) from different upper funneling aromatic pathways. The strain degraded four of these compounds at 10°C, 20°C, and 30°C; syringic acid and vanillic acid were not utilized as sole carbon source. The degradation of 5 mM and 10 mM ferulic acid was accompanied by the stable accumulation of high amounts of the value-added product vanillic acid (85–89% molar yield; 760 and 1540 mg l(−1), respectively) over the whole temperature range tested. The presence of essential genes required for reactions in the upper funneling pathways was confirmed in the genome. This is the first report on biodegradation of lignin monomers and the stable vanillic acid production at low and moderate temperatures by P. aromaticivorans. KEY POINTS: • Paraburkholderia aromaticivorans AR20-38 successfully degrades four lignin monomers. • Successful degradation study at low (10°C) and moderate temperatures (20–30°C). • Biotechnological value: high yield of vanillic acid produced from ferulic acid. |
format | Online Article Text |
id | pubmed-8007519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-80075192021-04-16 Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil Margesin, Rosa Volgger, Georg Wagner, Andreas O. Zhang, Dechao Poyntner, Caroline Appl Microbiol Biotechnol Environmental Biotechnology ABSTRACT: Lignin bio-valorization is an emerging field of applied biotechnology and has not yet been studied at low temperatures. Paraburkholderia aromaticivorans AR20-38 was examined for its potential to degrade six selected lignin monomers (syringic acid, p-coumaric acid, 4-hydroxybenzoic acid, ferulic acid, vanillic acid, benzoic acid) from different upper funneling aromatic pathways. The strain degraded four of these compounds at 10°C, 20°C, and 30°C; syringic acid and vanillic acid were not utilized as sole carbon source. The degradation of 5 mM and 10 mM ferulic acid was accompanied by the stable accumulation of high amounts of the value-added product vanillic acid (85–89% molar yield; 760 and 1540 mg l(−1), respectively) over the whole temperature range tested. The presence of essential genes required for reactions in the upper funneling pathways was confirmed in the genome. This is the first report on biodegradation of lignin monomers and the stable vanillic acid production at low and moderate temperatures by P. aromaticivorans. KEY POINTS: • Paraburkholderia aromaticivorans AR20-38 successfully degrades four lignin monomers. • Successful degradation study at low (10°C) and moderate temperatures (20–30°C). • Biotechnological value: high yield of vanillic acid produced from ferulic acid. Springer Berlin Heidelberg 2021-03-09 2021 /pmc/articles/PMC8007519/ /pubmed/33687503 http://dx.doi.org/10.1007/s00253-021-11215-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Environmental Biotechnology Margesin, Rosa Volgger, Georg Wagner, Andreas O. Zhang, Dechao Poyntner, Caroline Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title | Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title_full | Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title_fullStr | Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title_full_unstemmed | Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title_short | Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil |
title_sort | biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by paraburkholderia aromaticivorans ar20-38 isolated from alpine forest soil |
topic | Environmental Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007519/ https://www.ncbi.nlm.nih.gov/pubmed/33687503 http://dx.doi.org/10.1007/s00253-021-11215-z |
work_keys_str_mv | AT margesinrosa biodegradationofligninmonomersandbioconversionofferulicacidtovanillicacidbyparaburkholderiaaromaticivoransar2038isolatedfromalpineforestsoil AT volggergeorg biodegradationofligninmonomersandbioconversionofferulicacidtovanillicacidbyparaburkholderiaaromaticivoransar2038isolatedfromalpineforestsoil AT wagnerandreaso biodegradationofligninmonomersandbioconversionofferulicacidtovanillicacidbyparaburkholderiaaromaticivoransar2038isolatedfromalpineforestsoil AT zhangdechao biodegradationofligninmonomersandbioconversionofferulicacidtovanillicacidbyparaburkholderiaaromaticivoransar2038isolatedfromalpineforestsoil AT poyntnercaroline biodegradationofligninmonomersandbioconversionofferulicacidtovanillicacidbyparaburkholderiaaromaticivoransar2038isolatedfromalpineforestsoil |