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Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy

BACKGROUND AND OBJECTIVES: Immune thrombocytopenia (ITP) is one of the autoimmune diseases that presented by thrombocytopenia and increased risk of bleeding. Etiology of immune thrombocytopenia (ITP) is very complex. Lymphocyte function associated antigen-1 (LFA-1) plays important role in ITP. The a...

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Autores principales: Abdel Hameed, Muhamad R, Nafady, Howiada A, Mostafa, Mona Ibrahim, Sayed, Douaa, Obiedallah, Ahmad A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007592/
https://www.ncbi.nlm.nih.gov/pubmed/33790683
http://dx.doi.org/10.2147/JBM.S300717
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author Abdel Hameed, Muhamad R
Nafady, Howiada A
Mostafa, Mona Ibrahim
Sayed, Douaa
Obiedallah, Ahmad A
author_facet Abdel Hameed, Muhamad R
Nafady, Howiada A
Mostafa, Mona Ibrahim
Sayed, Douaa
Obiedallah, Ahmad A
author_sort Abdel Hameed, Muhamad R
collection PubMed
description BACKGROUND AND OBJECTIVES: Immune thrombocytopenia (ITP) is one of the autoimmune diseases that presented by thrombocytopenia and increased risk of bleeding. Etiology of immune thrombocytopenia (ITP) is very complex. Lymphocyte function associated antigen-1 (LFA-1) plays important role in ITP. The aim of this study was evaluation of expression of CD11a on lymphocytes to explore its possible role in primary ITP patients also, regarding severity and response to immunosuppressive treatment. PATIENTS AND METHODS: This is a cross-sectional case-control study. Forty adult patients aged (18:58) years, 29 females and 11 males were enrolled as newly diagnosed primary ITP. Forty age and sex matched control subjects were randomly selected. The expression of CD11a on lymphocyte subpopulations (CD3+ T cells, CD3+CD4+ T cells and CD19+ B cells) was analyzed by flowcytometry at the start of the study and after 6 months of follow-up. RESULTS: The mean fluorescence intensity (MFI) of CD11a on CD3+ T and CD19+ B lymphocytes was significantly highly increased in ITP patients compared to healthy controls while MFI of CD11a on CD3+ CD4+Tclls was non-significant. MFI of CD11a on CD3+ and CD19+ B lymphocytes showed non-significant elevation with platelet count or bleeding score. MFI of CD11a on CD3+ showed significant highly increased level in refractory ITP compared with responder cases. CONCLUSION: CD11a had possible role in the pathogenesis of ITP. Immunosuppressive therapy in ITP did not affect the level of CD11a expression on T and B lymphocytes. Levels of CD11a do not reflect the severity of ITP neither platelet count nor bleeding score. Increased MFI of CD11a in CD3+T lymphocytes of ITP patients may cause resistance to immunosuppressive therapy.
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spelling pubmed-80075922021-03-30 Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy Abdel Hameed, Muhamad R Nafady, Howiada A Mostafa, Mona Ibrahim Sayed, Douaa Obiedallah, Ahmad A J Blood Med Original Research BACKGROUND AND OBJECTIVES: Immune thrombocytopenia (ITP) is one of the autoimmune diseases that presented by thrombocytopenia and increased risk of bleeding. Etiology of immune thrombocytopenia (ITP) is very complex. Lymphocyte function associated antigen-1 (LFA-1) plays important role in ITP. The aim of this study was evaluation of expression of CD11a on lymphocytes to explore its possible role in primary ITP patients also, regarding severity and response to immunosuppressive treatment. PATIENTS AND METHODS: This is a cross-sectional case-control study. Forty adult patients aged (18:58) years, 29 females and 11 males were enrolled as newly diagnosed primary ITP. Forty age and sex matched control subjects were randomly selected. The expression of CD11a on lymphocyte subpopulations (CD3+ T cells, CD3+CD4+ T cells and CD19+ B cells) was analyzed by flowcytometry at the start of the study and after 6 months of follow-up. RESULTS: The mean fluorescence intensity (MFI) of CD11a on CD3+ T and CD19+ B lymphocytes was significantly highly increased in ITP patients compared to healthy controls while MFI of CD11a on CD3+ CD4+Tclls was non-significant. MFI of CD11a on CD3+ and CD19+ B lymphocytes showed non-significant elevation with platelet count or bleeding score. MFI of CD11a on CD3+ showed significant highly increased level in refractory ITP compared with responder cases. CONCLUSION: CD11a had possible role in the pathogenesis of ITP. Immunosuppressive therapy in ITP did not affect the level of CD11a expression on T and B lymphocytes. Levels of CD11a do not reflect the severity of ITP neither platelet count nor bleeding score. Increased MFI of CD11a in CD3+T lymphocytes of ITP patients may cause resistance to immunosuppressive therapy. Dove 2021-03-25 /pmc/articles/PMC8007592/ /pubmed/33790683 http://dx.doi.org/10.2147/JBM.S300717 Text en © 2021 Abdel Hameed et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Abdel Hameed, Muhamad R
Nafady, Howiada A
Mostafa, Mona Ibrahim
Sayed, Douaa
Obiedallah, Ahmad A
Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title_full Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title_fullStr Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title_full_unstemmed Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title_short Possible Role of CD11a in Primary Immune Thrombocytopenia Patients on Immunosuppressive Therapy
title_sort possible role of cd11a in primary immune thrombocytopenia patients on immunosuppressive therapy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007592/
https://www.ncbi.nlm.nih.gov/pubmed/33790683
http://dx.doi.org/10.2147/JBM.S300717
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