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A haemagglutination test for rapid detection of antibodies to SARS-CoV-2
Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require c...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007702/ https://www.ncbi.nlm.nih.gov/pubmed/33782398 http://dx.doi.org/10.1038/s41467-021-22045-y |
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| author | Townsend, Alain Rijal, Pramila Xiao, Julie Tan, Tiong Kit Huang, Kuan-Ying A. Schimanski, Lisa Huo, Jiandong Gupta, Nimesh Rahikainen, Rolle Matthews, Philippa C. Crook, Derrick Hoosdally, Sarah Dunachie, Susanna Barnes, Eleanor Street, Teresa Conlon, Christopher P. Frater, John Arancibia-Cárcamo, Carolina V. Rudkin, Justine Stoesser, Nicole Karpe, Fredrik Neville, Matthew Ploeg, Rutger Oliveira, Marta Roberts, David J. Lamikanra, Abigail A. Tsang, Hoi Pat Bown, Abbie Vipond, Richard Mentzer, Alexander J. Knight, Julian C. Kwok, Andrew J. Screaton, Gavin R. Mongkolsapaya, Juthathip Dejnirattisai, Wanwisa Supasa, Piyada Klenerman, Paul Dold, Christina Baillie, J. Kenneth Moore, Shona C. Openshaw, Peter J. M. Semple, Malcolm G. Turtle, Lance C. W. Ainsworth, Mark Allcock, Alice Beer, Sally Bibi, Sagida Skelly, Donal Stafford, Lizzy Jeffrey, Katie O’Donnell, Denise Clutterbuck, Elizabeth Espinosa, Alexis Mendoza, Maria Georgiou, Dominique Lockett, Teresa Martinez, Jose Perez, Elena Gallardo Sanchez, Veronica Scozzafava, Giuseppe Sobrinodiaz, Alberto Thraves, Hannah Joly, Etienne |
| author_facet | Townsend, Alain Rijal, Pramila Xiao, Julie Tan, Tiong Kit Huang, Kuan-Ying A. Schimanski, Lisa Huo, Jiandong Gupta, Nimesh Rahikainen, Rolle Matthews, Philippa C. Crook, Derrick Hoosdally, Sarah Dunachie, Susanna Barnes, Eleanor Street, Teresa Conlon, Christopher P. Frater, John Arancibia-Cárcamo, Carolina V. Rudkin, Justine Stoesser, Nicole Karpe, Fredrik Neville, Matthew Ploeg, Rutger Oliveira, Marta Roberts, David J. Lamikanra, Abigail A. Tsang, Hoi Pat Bown, Abbie Vipond, Richard Mentzer, Alexander J. Knight, Julian C. Kwok, Andrew J. Screaton, Gavin R. Mongkolsapaya, Juthathip Dejnirattisai, Wanwisa Supasa, Piyada Klenerman, Paul Dold, Christina Baillie, J. Kenneth Moore, Shona C. Openshaw, Peter J. M. Semple, Malcolm G. Turtle, Lance C. W. Ainsworth, Mark Allcock, Alice Beer, Sally Bibi, Sagida Skelly, Donal Stafford, Lizzy Jeffrey, Katie O’Donnell, Denise Clutterbuck, Elizabeth Espinosa, Alexis Mendoza, Maria Georgiou, Dominique Lockett, Teresa Martinez, Jose Perez, Elena Gallardo Sanchez, Veronica Scozzafava, Giuseppe Sobrinodiaz, Alberto Thraves, Hannah Joly, Etienne |
| author_sort | Townsend, Alain |
| collection | PubMed |
| description | Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world. |
| format | Online Article Text |
| id | pubmed-8007702 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-80077022021-04-16 A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 Townsend, Alain Rijal, Pramila Xiao, Julie Tan, Tiong Kit Huang, Kuan-Ying A. Schimanski, Lisa Huo, Jiandong Gupta, Nimesh Rahikainen, Rolle Matthews, Philippa C. Crook, Derrick Hoosdally, Sarah Dunachie, Susanna Barnes, Eleanor Street, Teresa Conlon, Christopher P. Frater, John Arancibia-Cárcamo, Carolina V. Rudkin, Justine Stoesser, Nicole Karpe, Fredrik Neville, Matthew Ploeg, Rutger Oliveira, Marta Roberts, David J. Lamikanra, Abigail A. Tsang, Hoi Pat Bown, Abbie Vipond, Richard Mentzer, Alexander J. Knight, Julian C. Kwok, Andrew J. Screaton, Gavin R. Mongkolsapaya, Juthathip Dejnirattisai, Wanwisa Supasa, Piyada Klenerman, Paul Dold, Christina Baillie, J. Kenneth Moore, Shona C. Openshaw, Peter J. M. Semple, Malcolm G. Turtle, Lance C. W. Ainsworth, Mark Allcock, Alice Beer, Sally Bibi, Sagida Skelly, Donal Stafford, Lizzy Jeffrey, Katie O’Donnell, Denise Clutterbuck, Elizabeth Espinosa, Alexis Mendoza, Maria Georgiou, Dominique Lockett, Teresa Martinez, Jose Perez, Elena Gallardo Sanchez, Veronica Scozzafava, Giuseppe Sobrinodiaz, Alberto Thraves, Hannah Joly, Etienne Nat Commun Article Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world. Nature Publishing Group UK 2021-03-29 /pmc/articles/PMC8007702/ /pubmed/33782398 http://dx.doi.org/10.1038/s41467-021-22045-y Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Townsend, Alain Rijal, Pramila Xiao, Julie Tan, Tiong Kit Huang, Kuan-Ying A. Schimanski, Lisa Huo, Jiandong Gupta, Nimesh Rahikainen, Rolle Matthews, Philippa C. Crook, Derrick Hoosdally, Sarah Dunachie, Susanna Barnes, Eleanor Street, Teresa Conlon, Christopher P. Frater, John Arancibia-Cárcamo, Carolina V. Rudkin, Justine Stoesser, Nicole Karpe, Fredrik Neville, Matthew Ploeg, Rutger Oliveira, Marta Roberts, David J. Lamikanra, Abigail A. Tsang, Hoi Pat Bown, Abbie Vipond, Richard Mentzer, Alexander J. Knight, Julian C. Kwok, Andrew J. Screaton, Gavin R. Mongkolsapaya, Juthathip Dejnirattisai, Wanwisa Supasa, Piyada Klenerman, Paul Dold, Christina Baillie, J. Kenneth Moore, Shona C. Openshaw, Peter J. M. Semple, Malcolm G. Turtle, Lance C. W. Ainsworth, Mark Allcock, Alice Beer, Sally Bibi, Sagida Skelly, Donal Stafford, Lizzy Jeffrey, Katie O’Donnell, Denise Clutterbuck, Elizabeth Espinosa, Alexis Mendoza, Maria Georgiou, Dominique Lockett, Teresa Martinez, Jose Perez, Elena Gallardo Sanchez, Veronica Scozzafava, Giuseppe Sobrinodiaz, Alberto Thraves, Hannah Joly, Etienne A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title | A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title_full | A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title_fullStr | A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title_full_unstemmed | A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title_short | A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 |
| title_sort | haemagglutination test for rapid detection of antibodies to sars-cov-2 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007702/ https://www.ncbi.nlm.nih.gov/pubmed/33782398 http://dx.doi.org/10.1038/s41467-021-22045-y |
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