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Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility

Background: The mechanism of Interleukin-17 (IL-17) induced ventricular arrhythmia (VA) remains unclear. This study aimed to investigate the effect of intracellular calcium (Ca(i)) handling and VA susceptibility by IL-17. Methods: The electrophysiological properties of isolated perfused rabbit heart...

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Autores principales: Tsai, Yung-Nan, Hsiao, Ya-Wen, Lin, Shien-Fong, Chan, Yi-Hsin, Hsieh, Yu-Cheng, Tang, Wei-Hua, Lee, An-Sheng, Huang, Yu-Ting, Li, Hsing-Yuan, Chao, Tze-Fan, Higa, Satoshi, Wu, Tsu-Juey, Chang, Shih-Lin, Chen, Shih-Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007768/
https://www.ncbi.nlm.nih.gov/pubmed/33796569
http://dx.doi.org/10.3389/fcvm.2021.623510
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author Tsai, Yung-Nan
Hsiao, Ya-Wen
Lin, Shien-Fong
Chan, Yi-Hsin
Hsieh, Yu-Cheng
Tang, Wei-Hua
Lee, An-Sheng
Huang, Yu-Ting
Li, Hsing-Yuan
Chao, Tze-Fan
Higa, Satoshi
Wu, Tsu-Juey
Chang, Shih-Lin
Chen, Shih-Ann
author_facet Tsai, Yung-Nan
Hsiao, Ya-Wen
Lin, Shien-Fong
Chan, Yi-Hsin
Hsieh, Yu-Cheng
Tang, Wei-Hua
Lee, An-Sheng
Huang, Yu-Ting
Li, Hsing-Yuan
Chao, Tze-Fan
Higa, Satoshi
Wu, Tsu-Juey
Chang, Shih-Lin
Chen, Shih-Ann
author_sort Tsai, Yung-Nan
collection PubMed
description Background: The mechanism of Interleukin-17 (IL-17) induced ventricular arrhythmia (VA) remains unclear. This study aimed to investigate the effect of intracellular calcium (Ca(i)) handling and VA susceptibility by IL-17. Methods: The electrophysiological properties of isolated perfused rabbit hearts under IL-17 (20 ng/ml, N = 6) and the IL-17 with neutralizer (0.4 μg/ml, N = 6) were evaluated using an optical mapping system. The action potential duration (APD) and Ca(i) transient duration (Ca(i)TD) were examined, and semiquantitative reverse transcriptase-polymerase chain reaction analysis of ion channels was performed. Results: There were longer APD(80), Ca(i)TD(80) and increased thresholds of APD and Ca(i)TD alternans, the maximum slope of APD restitution and induction of VA threshold in IL-17 group compared with those in IL-17 neutralizer and baseline groups. During ventricular fibrillation, the number of phase singularities and dominant frequency were both significantly greater in IL-17 group than in baseline group. The mRNA expressions of the Na(+)/Ca(2+) exchanger, phospholamban, and ryanodine receptor Ca(2+) release channel were upregulated, and the subunit of L-type Ca(2+) current and sarcoplasmic reticulum Ca(2+)-ATPase 2a were significantly reduced in IL-17 group compared to baseline and IL-17 neutralizer group. Conclusions: IL-17 enhanced Ca(i)TD and APD alternans through disturbances in calcium handling, which may increase VA susceptibility.
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spelling pubmed-80077682021-03-31 Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility Tsai, Yung-Nan Hsiao, Ya-Wen Lin, Shien-Fong Chan, Yi-Hsin Hsieh, Yu-Cheng Tang, Wei-Hua Lee, An-Sheng Huang, Yu-Ting Li, Hsing-Yuan Chao, Tze-Fan Higa, Satoshi Wu, Tsu-Juey Chang, Shih-Lin Chen, Shih-Ann Front Cardiovasc Med Cardiovascular Medicine Background: The mechanism of Interleukin-17 (IL-17) induced ventricular arrhythmia (VA) remains unclear. This study aimed to investigate the effect of intracellular calcium (Ca(i)) handling and VA susceptibility by IL-17. Methods: The electrophysiological properties of isolated perfused rabbit hearts under IL-17 (20 ng/ml, N = 6) and the IL-17 with neutralizer (0.4 μg/ml, N = 6) were evaluated using an optical mapping system. The action potential duration (APD) and Ca(i) transient duration (Ca(i)TD) were examined, and semiquantitative reverse transcriptase-polymerase chain reaction analysis of ion channels was performed. Results: There were longer APD(80), Ca(i)TD(80) and increased thresholds of APD and Ca(i)TD alternans, the maximum slope of APD restitution and induction of VA threshold in IL-17 group compared with those in IL-17 neutralizer and baseline groups. During ventricular fibrillation, the number of phase singularities and dominant frequency were both significantly greater in IL-17 group than in baseline group. The mRNA expressions of the Na(+)/Ca(2+) exchanger, phospholamban, and ryanodine receptor Ca(2+) release channel were upregulated, and the subunit of L-type Ca(2+) current and sarcoplasmic reticulum Ca(2+)-ATPase 2a were significantly reduced in IL-17 group compared to baseline and IL-17 neutralizer group. Conclusions: IL-17 enhanced Ca(i)TD and APD alternans through disturbances in calcium handling, which may increase VA susceptibility. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8007768/ /pubmed/33796569 http://dx.doi.org/10.3389/fcvm.2021.623510 Text en Copyright © 2021 Tsai, Hsiao, Lin, Chan, Hsieh, Tang, Lee, Huang, Li, Chao, Higa, Wu, Chang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Tsai, Yung-Nan
Hsiao, Ya-Wen
Lin, Shien-Fong
Chan, Yi-Hsin
Hsieh, Yu-Cheng
Tang, Wei-Hua
Lee, An-Sheng
Huang, Yu-Ting
Li, Hsing-Yuan
Chao, Tze-Fan
Higa, Satoshi
Wu, Tsu-Juey
Chang, Shih-Lin
Chen, Shih-Ann
Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title_full Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title_fullStr Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title_full_unstemmed Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title_short Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility
title_sort proinflammatory cytokine modulates intracellular calcium handling and enhances ventricular arrhythmia susceptibility
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007768/
https://www.ncbi.nlm.nih.gov/pubmed/33796569
http://dx.doi.org/10.3389/fcvm.2021.623510
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