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Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study

Monomeric C-reactive protein (mCRP) is now accepted as having a key role in modulating inflammation and in particular, has been strongly associated with atherosclerotic arterial plaque progression and instability and neuroinflammation after stroke where a build-up of the mCRP protein within the brai...

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Autores principales: Al-Baradie, Raid S., Pu, Shuang, Liu, Donghui, Zeinolabediny, Yasmin, Ferris, Glenn, Sanfeli, Coral, Corpas, Ruben, Garcia-Lara, Elisa, Alsagaby, Suliman A., Alshehri, Bader M., Abdel-hadi, Ahmed M., Ahmad, Fuzail, Moatari, Psalm, Heidari, Nima, Slevin, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007856/
https://www.ncbi.nlm.nih.gov/pubmed/33796111
http://dx.doi.org/10.3389/fimmu.2021.644213
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author Al-Baradie, Raid S.
Pu, Shuang
Liu, Donghui
Zeinolabediny, Yasmin
Ferris, Glenn
Sanfeli, Coral
Corpas, Ruben
Garcia-Lara, Elisa
Alsagaby, Suliman A.
Alshehri, Bader M.
Abdel-hadi, Ahmed M.
Ahmad, Fuzail
Moatari, Psalm
Heidari, Nima
Slevin, Mark
author_facet Al-Baradie, Raid S.
Pu, Shuang
Liu, Donghui
Zeinolabediny, Yasmin
Ferris, Glenn
Sanfeli, Coral
Corpas, Ruben
Garcia-Lara, Elisa
Alsagaby, Suliman A.
Alshehri, Bader M.
Abdel-hadi, Ahmed M.
Ahmad, Fuzail
Moatari, Psalm
Heidari, Nima
Slevin, Mark
author_sort Al-Baradie, Raid S.
collection PubMed
description Monomeric C-reactive protein (mCRP) is now accepted as having a key role in modulating inflammation and in particular, has been strongly associated with atherosclerotic arterial plaque progression and instability and neuroinflammation after stroke where a build-up of the mCRP protein within the brain parenchyma appears to be connected to vascular damage, neurodegenerative pathophysiology and possibly Alzheimer's Disease (AD) and dementia. Here, using immunohistochemical analysis, we wanted to confirm mCRP localization and overall distribution within a cohort of AD patients showing evidence of previous infarction and then focus on its co-localization with inflammatory active regions in order to provide further evidence of its functional and direct impact. We showed that mCRP was particularly seen in large amounts within brain vessels of all sizes and that the immediate micro-environment surrounding these had become laden with mCRP positive cells and extra cellular matrix. This suggested possible leakage and transport into the local tissue. The mCRP-positive regions were almost always associated with neurodegenerative, damaged tissue as hallmarked by co-positivity with pTau and β-amyloid staining. Where this occurred, cells with the morphology of neurons, macrophages and glia, as well as smaller microvessels became mCRP-positive in regions staining for the inflammatory markers CD68 (macrophage), interleukin-1 beta (IL-1β) and nuclear factor kappa B (NFκB), showing evidence of a perpetuation of inflammation. Positive staining for mCRP was seen even in distant hypothalamic regions. In conclusion, brain injury or inflammatory neurodegenerative processes are strongly associated with mCRP localization within the tissue and given our knowledge of its biological properties, it is likely that this protein plays a direct role in promoting tissue damage and supporting progression of AD after injury.
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spelling pubmed-80078562021-03-31 Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study Al-Baradie, Raid S. Pu, Shuang Liu, Donghui Zeinolabediny, Yasmin Ferris, Glenn Sanfeli, Coral Corpas, Ruben Garcia-Lara, Elisa Alsagaby, Suliman A. Alshehri, Bader M. Abdel-hadi, Ahmed M. Ahmad, Fuzail Moatari, Psalm Heidari, Nima Slevin, Mark Front Immunol Immunology Monomeric C-reactive protein (mCRP) is now accepted as having a key role in modulating inflammation and in particular, has been strongly associated with atherosclerotic arterial plaque progression and instability and neuroinflammation after stroke where a build-up of the mCRP protein within the brain parenchyma appears to be connected to vascular damage, neurodegenerative pathophysiology and possibly Alzheimer's Disease (AD) and dementia. Here, using immunohistochemical analysis, we wanted to confirm mCRP localization and overall distribution within a cohort of AD patients showing evidence of previous infarction and then focus on its co-localization with inflammatory active regions in order to provide further evidence of its functional and direct impact. We showed that mCRP was particularly seen in large amounts within brain vessels of all sizes and that the immediate micro-environment surrounding these had become laden with mCRP positive cells and extra cellular matrix. This suggested possible leakage and transport into the local tissue. The mCRP-positive regions were almost always associated with neurodegenerative, damaged tissue as hallmarked by co-positivity with pTau and β-amyloid staining. Where this occurred, cells with the morphology of neurons, macrophages and glia, as well as smaller microvessels became mCRP-positive in regions staining for the inflammatory markers CD68 (macrophage), interleukin-1 beta (IL-1β) and nuclear factor kappa B (NFκB), showing evidence of a perpetuation of inflammation. Positive staining for mCRP was seen even in distant hypothalamic regions. In conclusion, brain injury or inflammatory neurodegenerative processes are strongly associated with mCRP localization within the tissue and given our knowledge of its biological properties, it is likely that this protein plays a direct role in promoting tissue damage and supporting progression of AD after injury. Frontiers Media S.A. 2021-03-16 /pmc/articles/PMC8007856/ /pubmed/33796111 http://dx.doi.org/10.3389/fimmu.2021.644213 Text en Copyright © 2021 Al-Baradie, Pu, Liu, Zeinolabediny, Ferris, Sanfeli, Corpas, Garcia-Lara, Alsagaby, Alshehri, Abdel-hadi, Ahmad, Moatari, Heidari and Slevin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Al-Baradie, Raid S.
Pu, Shuang
Liu, Donghui
Zeinolabediny, Yasmin
Ferris, Glenn
Sanfeli, Coral
Corpas, Ruben
Garcia-Lara, Elisa
Alsagaby, Suliman A.
Alshehri, Bader M.
Abdel-hadi, Ahmed M.
Ahmad, Fuzail
Moatari, Psalm
Heidari, Nima
Slevin, Mark
Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title_full Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title_fullStr Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title_full_unstemmed Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title_short Monomeric C-Reactive Protein Localized in the Cerebral Tissue of Damaged Vascular Brain Regions Is Associated With Neuro-Inflammation and Neurodegeneration-An Immunohistochemical Study
title_sort monomeric c-reactive protein localized in the cerebral tissue of damaged vascular brain regions is associated with neuro-inflammation and neurodegeneration-an immunohistochemical study
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007856/
https://www.ncbi.nlm.nih.gov/pubmed/33796111
http://dx.doi.org/10.3389/fimmu.2021.644213
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